Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias)

Lauren E Smith, Kathryn Crouch, Wei Cao, Mischa R Müller, Leeying Wu, John Steven, Michael Lee, Musen Liang, Martin F Flajnik, Heather H Shih, Caroline Barelle, Janet Paulsen, Davinder S Gill, Helen Dooley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain.
Original languageEnglish
Pages (from-to)665-679
Number of pages15
JournalDevelopmental and Comparative Immunology
Volume36
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Squalus acanthias
Sharks
Immunoglobulin M
Immunoglobulins
Spliced Leader RNA
Immunoglobulin D
Serum
Sequence Analysis
Blood Proteins
Mammals
Immune System
Fishes
Complementary DNA
Antigens
Proteins

Keywords

  • evolution
  • cartilaginous fish
  • adaptive immunity
  • immunoglobulin
  • IgM
  • IgNAR
  • IgD

Cite this

Smith, L. E., Crouch, K., Cao, W., Müller, M. R., Wu, L., Steven, J., ... Dooley, H. (2012). Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). Developmental and Comparative Immunology, 36(4), 665-679. https://doi.org/10.1016/j.dci.2011.10.007

Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). / Smith, Lauren E; Crouch, Kathryn; Cao, Wei; Müller, Mischa R; Wu, Leeying; Steven, John; Lee, Michael; Liang, Musen; Flajnik, Martin F; Shih, Heather H; Barelle, Caroline; Paulsen, Janet; Gill, Davinder S; Dooley, Helen.

In: Developmental and Comparative Immunology, Vol. 36, No. 4, 04.2012, p. 665-679.

Research output: Contribution to journalArticle

Smith, LE, Crouch, K, Cao, W, Müller, MR, Wu, L, Steven, J, Lee, M, Liang, M, Flajnik, MF, Shih, HH, Barelle, C, Paulsen, J, Gill, DS & Dooley, H 2012, 'Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias)', Developmental and Comparative Immunology, vol. 36, no. 4, pp. 665-679. https://doi.org/10.1016/j.dci.2011.10.007
Smith, Lauren E ; Crouch, Kathryn ; Cao, Wei ; Müller, Mischa R ; Wu, Leeying ; Steven, John ; Lee, Michael ; Liang, Musen ; Flajnik, Martin F ; Shih, Heather H ; Barelle, Caroline ; Paulsen, Janet ; Gill, Davinder S ; Dooley, Helen. / Characterization of the immunoglobulin repertoire of the spiny dogfish (Squalus acanthias). In: Developmental and Comparative Immunology. 2012 ; Vol. 36, No. 4. pp. 665-679.
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AU - Lee, Michael

AU - Liang, Musen

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AU - Shih, Heather H

AU - Barelle, Caroline

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AU - Dooley, Helen

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N2 - The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain.

AB - The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein levels. Despite the presence of hundreds of IgM clusters in this species the serum levels of this isotype are comparatively low. However, analysis of cDNA sequences and serum protein suggests microheterogeneity in the IgM heavy chains and supports the proposal that different clusters are preferentially used in the two forms (monomer or pentamer) of this isotype. We also found that the IgNAR isotype in this species exists in a previously unknown multimeric format in serum. Finally, we identified a new form of the IgW isotype (the shark IgD orthologue), in which the leader is spliced directly to the first constant domain, resulting in a molecule lacking an antigen-binding domain.

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