Characterization of the testicular, epididymal and endocrine phenotypes in the Leuven Vdr-deficient mouse model: targeting estrogen signalling

Martin Blomberg Jensen, Liesbet Lieben, John E. Nielsen, Ariane Willems, Anne Jørgensen, Anders Juul, Jorma Toppari, Geert Carmeliet, Ewa Rajpert-De Meyts

Research output: Contribution to journalArticle

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Abstract

Vitamin D is a key factor for calcium and bone homeostasis, but signalling through the vitamin D receptor (VDR) seems also to be important for testicular function. To test the functional role of vitamin D signalling we examined the male reproductive system of the Leuven Vdr-ablated (Vdr(-/-)) mice, previously established as a model for hereditary vitamin D-resistant rickets. We investigated reproductive hormones, changes in gene expression and histological phenotype of eleven Vdr(-/-), eight Vdr(+/-) and nine Vdr(+/+) mice. Testicular and epididymal histology were grossly normal in Vdr(-/-) mice. Accordingly, no differences were found in serum concentrations of testosterone, estradiol, LH, and FSH or testicular expression of Cyp19a1, Ersα, Cyp17a1, Star, Insl3, Inhbb, and Amh. However, a significantly lower ERβ expression was found in testis of Vdr(+/-) and Vdr(-/-) mice, conversely epididymal expressions of ERα and the estrogen-target gene Aqp9 were higher. In conclusion, vitamin D seems dispensable for murine spermatogenesis and sex hormone production, but aberrant estrogen-signalling may elicit some of the VDR-mediated effects on male reproduction.
Original languageEnglish
Pages (from-to)93-102
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume377
Issue number1-2
Early online date10 Jul 2013
DOIs
Publication statusPublished - 5 Sep 2013

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Vitamin D
Estrogens
Phenotype
Calcitriol Receptors
Hypophosphatemic Rickets
Histology
Gonadal Steroid Hormones
Spermatogenesis
Gene expression
Stars
Reproduction
Testosterone
Testis
Estradiol
Bone
Homeostasis
Genes
Hormones
Calcium
Gene Expression

Keywords

  • Vitamin D
  • Testis
  • Fertility
  • Reproduction
  • Sex hormones
  • Estrogen

Cite this

Characterization of the testicular, epididymal and endocrine phenotypes in the Leuven Vdr-deficient mouse model : targeting estrogen signalling. / Blomberg Jensen, Martin; Lieben, Liesbet; Nielsen, John E.; Willems, Ariane; Jørgensen, Anne; Juul, Anders; Toppari, Jorma; Carmeliet, Geert; Rajpert-De Meyts, Ewa.

In: Molecular and Cellular Endocrinology, Vol. 377, No. 1-2, 05.09.2013, p. 93-102.

Research output: Contribution to journalArticle

Blomberg Jensen, M, Lieben, L, Nielsen, JE, Willems, A, Jørgensen, A, Juul, A, Toppari, J, Carmeliet, G & Rajpert-De Meyts, E 2013, 'Characterization of the testicular, epididymal and endocrine phenotypes in the Leuven Vdr-deficient mouse model: targeting estrogen signalling', Molecular and Cellular Endocrinology, vol. 377, no. 1-2, pp. 93-102. https://doi.org/10.1016/j.mce.2013.06.036
Blomberg Jensen, Martin ; Lieben, Liesbet ; Nielsen, John E. ; Willems, Ariane ; Jørgensen, Anne ; Juul, Anders ; Toppari, Jorma ; Carmeliet, Geert ; Rajpert-De Meyts, Ewa. / Characterization of the testicular, epididymal and endocrine phenotypes in the Leuven Vdr-deficient mouse model : targeting estrogen signalling. In: Molecular and Cellular Endocrinology. 2013 ; Vol. 377, No. 1-2. pp. 93-102.
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