Chemopreventive effect of the non-psychotropic phytocannabinoid cannabidiol on experimental colon cancer

Gabriella Aviello, Barbara Romano, Francesca Borrelli, Raffaele Capasso, Laura Gallo, Fabiana Piscitelli, Vincenzo Di Marzo, Angelo A Izzo

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Colon cancer affects millions of individuals in Western countries. Cannabidiol, a safe and non-psychotropic ingredient of Cannabis sativa, exerts pharmacological actions (antioxidant and intestinal antinflammatory) and mechanisms (inhibition of endocannabinoid enzymatic degradation) potentially beneficial for colon carcinogenesis. Thus, we investigated its possible chemopreventive effect in the model of colon cancer induced by azoxymethane (AOM) in mice. AOM treatment was associated with aberrant crypt foci (ACF, preneoplastic lesions), polyps, and tumour formation, up-regulation of phospho-Akt, iNOS and COX-2 and down-regulation of caspase-3. Cannabidiol-reduced ACF, polyps and tumours and counteracted AOM-induced phospho-Akt and caspase-3 changes. In colorectal carcinoma cell lines, cannabidiol protected DNA from oxidative damage, increased endocannabinoid levels and reduced cell proliferation in a CB(1)-, TRPV1- and PPARγ-antagonists sensitive manner. It is concluded that cannabidiol exerts chemopreventive effect in vivo and reduces cell proliferation through multiple mechanisms.

Original languageEnglish
Pages (from-to)925-934
Number of pages10
JournalJournal of Molecular Medicine
Volume90
Issue number8
Early online date10 Jan 2012
DOIs
Publication statusPublished - Aug 2012

Keywords

  • Animals
  • Antineoplastic Agents
  • Azoxymethane
  • Caco-2 Cells
  • Cannabidiol
  • Cell Line, Tumor
  • Cell Proliferation
  • Colonic Neoplasms
  • Comet Assay
  • HCT116 Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred ICR
  • Journal Article
  • Research Support, Non-U.S. Gov't

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  • Cite this

    Aviello, G., Romano, B., Borrelli, F., Capasso, R., Gallo, L., Piscitelli, F., Di Marzo, V., & Izzo, A. A. (2012). Chemopreventive effect of the non-psychotropic phytocannabinoid cannabidiol on experimental colon cancer. Journal of Molecular Medicine, 90(8), 925-934. https://doi.org/10.1007/s00109-011-0856-x