Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene

Krystyna Cwiklinski, Diana Meskill, Mark W Robinson, Eduardo Pozio, Judith A Appleton, Bernadette Connolly

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Nematode parasites of the genus Trichinella are intracellular and distinct life cycle stages invade intestinal epithelial and skeletal muscle cells. Within the genus, Trichinella spiralis and Trichinella pseudospiralis exhibit species-specific differences with respect to host-parasite complex formation and host immune modulation. Parasite excretory-secretory (ES) proteins play important roles at the host-parasite interface and are thought to underpin these differences in biology. Serine proteases are among the most abundant group of T. spiralis ES proteins and multiple isoforms of the muscle larvae-specific TspSP-1 serine protease have been identified. Recently, a similar protein (TppSP-1) in T. pseudospiralis muscle larvae was identified. Here we report the cloning and characterisation of the full-length transcript of TppSP-1 and present comparative data between TspSP-1 and TppSP-1.
Original languageEnglish
Pages (from-to)268-271
Number of pages4
JournalVeterinary Parasitology
Volume159
Issue number3-4
Early online date22 Oct 2008
DOIs
Publication statusPublished - 23 Feb 2009

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Trichinella pseudospiralis
Trichinella
muscle larvae
Serine Proteases
serine proteinases
Larva
Organism Cloning
molecular cloning
Parasites
excretory-secretory products
Trichinella spiralis
parasites
Muscles
Genes
genes
Life Cycle Stages
myocytes
Muscle Cells
skeletal muscle
Protein Isoforms

Keywords

  • amino acid sequence
  • animals
  • cloning, molecular
  • gene expression regulation, enzymologic
  • larva
  • mice
  • molecular sequence data
  • muscle, skeletal
  • serine proteases
  • trichinella
  • trichinella spiralis
  • trichinella pseudospiralis
  • excretory/secretory proteins
  • western blot analysis
  • serine protease

Cite this

Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene. / Cwiklinski, Krystyna; Meskill, Diana; Robinson, Mark W; Pozio, Eduardo; Appleton, Judith A; Connolly, Bernadette.

In: Veterinary Parasitology, Vol. 159, No. 3-4, 23.02.2009, p. 268-271.

Research output: Contribution to journalArticle

Cwiklinski, Krystyna ; Meskill, Diana ; Robinson, Mark W ; Pozio, Eduardo ; Appleton, Judith A ; Connolly, Bernadette. / Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene. In: Veterinary Parasitology. 2009 ; Vol. 159, No. 3-4. pp. 268-271.
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AU - Meskill, Diana

AU - Robinson, Mark W

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AU - Appleton, Judith A

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KW - amino acid sequence

KW - animals

KW - cloning, molecular

KW - gene expression regulation, enzymologic

KW - larva

KW - mice

KW - molecular sequence data

KW - muscle, skeletal

KW - serine proteases

KW - trichinella

KW - trichinella spiralis

KW - trichinella pseudospiralis

KW - excretory/secretory proteins

KW - western blot analysis

KW - serine protease

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