Abstract
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) I and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145 bp and a 3' UTR of 481 bp. The IRF-2 cDNA contains a 1035 bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146 bp and a 3' UTR of 925 bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection I week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C. (C) 2002 Elsevier Science Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 111-126 |
| Number of pages | 15 |
| Journal | Developmental and Comparative Immunology |
| Volume | 27 |
| DOIs | |
| Publication status | Published - 2003 |
Keywords
- Interferon regulatory factor 1
- Interferon regulatory factor 2
- rainbow trout
- rainbow trout gonad
- DNA vaccination
- viral haemorrhagic septicaemia virus
- HEMORRHAGIC SEPTICEMIA VIRUS
- TRANSCRIPTION FACTOR IRF-1
- FACTOR-I IRF-1
- GROWTH-INHIBITION
- MOLECULAR-CLONING
- INDUCIBLE GENES
- CELL-GROWTH
- MX-PROTEIN
- DNA-DAMAGE
- APOPTOSIS
