Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).

Christopher John Secombes

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Abstract

Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) I and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145 bp and a 3' UTR of 481 bp. The IRF-2 cDNA contains a 1035 bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146 bp and a 3' UTR of 925 bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection I week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C. (C) 2002 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)111-126
Number of pages15
JournalDevelopmental and Comparative Immunology
Volume27
DOIs
Publication statusPublished - 2003

Keywords

  • Interferon regulatory factor 1
  • Interferon regulatory factor 2
  • rainbow trout
  • rainbow trout gonad
  • DNA vaccination
  • viral haemorrhagic septicaemia virus
  • HEMORRHAGIC SEPTICEMIA VIRUS
  • TRANSCRIPTION FACTOR IRF-1
  • FACTOR-I IRF-1
  • GROWTH-INHIBITION
  • MOLECULAR-CLONING
  • INDUCIBLE GENES
  • CELL-GROWTH
  • MX-PROTEIN
  • DNA-DAMAGE
  • APOPTOSIS

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