Cloning and expression analysis of the transforming growth factor-beta receptors type 1 and 2 in the rainbow trout Oncorhynchus mykiss

Tanja Maehr, Tiehui Wang, Jose L González Vecino, Simon Wadsworth, Christopher J Secombes

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Transforming growth factor-β (TGF-β) binding to the TGF-β type I (TGFBR1) and type II (TGFBR2) receptors delivers a plethora of cell-type specific effects. Moreover, the responses to TGF-β are tuned by regulatory mechanisms at the receptor level itself. To further elucidate TGF-β family signal transduction in teleosts, we therefore cloned the first complete set of a putative TGF-β receptor complex in salmonids. Rainbow trout TGFBR1 and TGFBR2 are transmembrane proteins with a serine/threonine kinase domain and are highly conserved within vertebrates. High expression levels in muscle and brain indicate regulation of the TGF-β system in muscular and nervous systems. Lipopolysaccharide (LPS) induced expression of both receptor chains in RTgill cells while bacterial and viral mimics modulated the two receptors inversely in head kidney (HK) macrophages. In addition, T cell mitogens lowered receptor levels in HK leukocytes. These data provide the first insights into TGF-β type I and II receptor modulation during immune responses in teleost fish.
Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume37
Issue number1
Early online date23 Oct 2011
DOIs
Publication statusPublished - May 2012

Fingerprint

Oncorhynchus mykiss
Transforming Growth Factors
Organism Cloning
Head Kidney
Mitogen Receptors
Salmonidae
Growth Factor Receptors
Protein-Serine-Threonine Kinases
TGF-beta type I receptor
Nervous System
Lipopolysaccharides
Vertebrates
Signal Transduction
Fishes
Leukocytes
Macrophages
T-Lymphocytes
Muscles
Brain
Proteins

Keywords

  • amino acid sequence
  • animals
  • brain
  • cells, cultured
  • cloning, molecular
  • conserved sequence
  • gene expression
  • gene expression regulation
  • head kidney
  • interferon inducers
  • leukocytes
  • lipopolysaccharides
  • macrophages
  • molecular sequence data
  • muscles
  • Oncorhynchus mykiss
  • organ specificity
  • phylogeny
  • poly I-C
  • receptors, transforming growth factor beta
  • sequence analysis, DNA
  • sequence homology, amino acid

Cite this

Cloning and expression analysis of the transforming growth factor-beta receptors type 1 and 2 in the rainbow trout Oncorhynchus mykiss. / Maehr, Tanja; Wang, Tiehui; González Vecino, Jose L; Wadsworth, Simon; Secombes, Christopher J.

In: Developmental and Comparative Immunology, Vol. 37, No. 1, 05.2012, p. 115-126.

Research output: Contribution to journalArticle

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