Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor

Perry Barrett, S Conway, R Jockers, A D Strosberg, B GuardiolaLemaitre, P Delagrange, Peter John Morgan

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We have isolated a novel variant of the Mel la melatonin receptor from an ovine PT cDNA library. Relative to the reported sequence for the Mel la melatonin receptor there are 8 changes in the DNA sequence. Only 3 of these result in amino acid substitutions, one in extracellular loop 3 and two in the carboxy-terminal tail. We have designated the novel variant of the sheep Mel la receptor Mel 1a(beta), and correspondingly the previously reported variant Mel la(alpha). As minor changes in the primary amino acid sequence of G-protein-coupled receptors can influence their functional characteristics we have accordingly characterized this novel variant of the Mel 1a melatonin receptor. This melatonin receptor displays high affinity binding and inhibits the cAMP second messenger pathway in transfected L-celIs demonstrating that this receptor is fully functional. PCR analysis shows Mel 1a(beta) is present in several breeds of sheep and suggests that the Mel 1a(beta) receptor was established early in the evolution of the sheep species. (C) 1997 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalBiochimica et Biophysica Acta. Molecular Cell Research
Volume1356
Issue number3
DOIs
Publication statusPublished - 27 May 1997

Keywords

  • pars tuberalis
  • mutation
  • isoform
  • allele
  • G protein
  • protein-coupled receptors
  • BETA(2)-adrnergic receptor
  • phodopus-sungorus
  • mutations
  • duration
  • brain

Cite this

Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor. / Barrett, Perry; Conway, S ; Jockers, R ; Strosberg, A D ; GuardiolaLemaitre, B ; Delagrange, P ; Morgan, Peter John.

In: Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1356, No. 3, 27.05.1997, p. 299-307.

Research output: Contribution to journalArticle

Barrett, Perry ; Conway, S ; Jockers, R ; Strosberg, A D ; GuardiolaLemaitre, B ; Delagrange, P ; Morgan, Peter John. / Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor. In: Biochimica et Biophysica Acta. Molecular Cell Research. 1997 ; Vol. 1356, No. 3. pp. 299-307.
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AU - Delagrange, P

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AB - We have isolated a novel variant of the Mel la melatonin receptor from an ovine PT cDNA library. Relative to the reported sequence for the Mel la melatonin receptor there are 8 changes in the DNA sequence. Only 3 of these result in amino acid substitutions, one in extracellular loop 3 and two in the carboxy-terminal tail. We have designated the novel variant of the sheep Mel la receptor Mel 1a(beta), and correspondingly the previously reported variant Mel la(alpha). As minor changes in the primary amino acid sequence of G-protein-coupled receptors can influence their functional characteristics we have accordingly characterized this novel variant of the Mel 1a melatonin receptor. This melatonin receptor displays high affinity binding and inhibits the cAMP second messenger pathway in transfected L-celIs demonstrating that this receptor is fully functional. PCR analysis shows Mel 1a(beta) is present in several breeds of sheep and suggests that the Mel 1a(beta) receptor was established early in the evolution of the sheep species. (C) 1997 Elsevier Science B.V.

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