Coexpression of leptin receptor and preproneuropeptide Y mRNA in arcuate nucleus of mouse hypothalamus

Julian Mercer, Nigel Hoggard, Lynda Williams, C B Lawrence, L T Hannah, Peter John Morgan, P Trayhurn

Research output: Contribution to journalArticle

406 Citations (Scopus)

Abstract

Leptin, the protein product of the adipose tissue-specific ob (obese) gene (1), reduces the body weight, adiposity and food intake of obese ob/ob mice on peripheral or central injection (2, 3, 4), [I-125]leptin binding has been detected in mouse choroid plexus (5), from which a leptin receptor gene was expression cloned (5). The gene has at least 6 splice variants (6, 7), Leptin receptor mRNA was localized in the hypothalamus by in situ hybridization being particularly abundantly expressed in the arcuate nucleus (8), There is evidence linking the physiological effects of injected leptin with hypothalamic neuropeptide Y (9, 10) (NPY), which has potent central effects on food intake and energy balance (11), and is also expressed in the arcuate nucleus. Here we report dual in situ hybridization studies for leptin receptor and NPY gene expression in the mouse arcuate nucleus, where the majority of cells examined expressed both genes. This provides the first direct evidence that leptin acts on cells that express NPY mRNA.

Original languageEnglish
Pages (from-to)733-735
Number of pages3
JournalJournal of Neuroendocrinology
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 1996

Keywords

  • leptin receptor mRNA
  • neuropeptide Y mRNA
  • arcuate nucleus
  • OBESE GENE-PRODUCT
  • NEUROPEPTIDE-Y
  • PROTEIN

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