Abstract
Background and Objectives This report describes the production of a freeze-dried preparation of pooled human plasma, containing immunoglobulin G (IgG) antibodies against the human platelet antigen 1a (HPA-1a). The material, coded 03/152, is proposed as an International Standard containing 100 arbitrary units of anti-HPA-1a for use in quantitative assays to determine the anti-HPA-1a activity in clinical samples.
Materials and Methods Plasma samples containing potent anti-HPA-1a were pooled and freeze dried in 1-ml ampoules. In addition, three individual plasma samples were selected which had varying levels of anti-HPA-1a activity. The anti-HPA-1a activity of these three samples was determined by using a variety of quantitative assays with the proposed standard as a reference.
Results An international collaborative study, which was part of the 2004 ISBT Platelet Immunology Workshop, involved 39 laboratories in 24 countries and showed that the anti-HPA-1a activity in three test samples could be reliably determined by using the proposed standard.
Conclusions Laboratories can use this standard to measure the anti-HPA-1a activity in patient's samples. Further studies are required to determine the relationship between anti-HPA-1a activity and clinical outcome in patients with neonatal alloimmune thrombocytopenia (NAIT).
Original language | English |
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Pages (from-to) | 100-104 |
Number of pages | 4 |
Journal | Vox Sanguinis |
Volume | 89 |
DOIs | |
Publication status | Published - 2005 |
Keywords
- anti-HPA-1a
- NAIT
- platelet alloantibody detection
- quantitation
- NEONATAL ALLOIMMUNE THROMBOCYTOPENIA
- ANTI-D
- BIOLOGICAL STANDARDS
- IMMUNOGLOBULIN
- ANTIBODIES
- MANAGEMENT
- EVALUATE
- NEWBORN
- 1A