Comet assay in human biomonitoring studies: Reliability, validation, and applications

A Collins, M Dusinska, M Franklin, M Somorovska, H Petrovska, S Duthie, L Fillion, M Panayiotidis, K Raslova, N Vaughan

Research output: Contribution to journalArticle

504 Citations (Scopus)

Abstract

The comet assay (single-cell gel electrophoresis), which measures DNA strand breaks at the level of single cells, is very easily applied to human lymphocytes, and therefore lends itself to human biomonitoring studies. For the examination of DNA bose oxidation (a specific marker of oxidative damage), the assay is modified by including a stage at which the DNA is incubated with a suitable lesion-specific endonuclease. Here we report on the reliability and reproducibility of this approach, From the level of comparing results from duplicate gels prepared from the same sample of cells, up to an assessment of the natural intra-and interindividual variability in lymphocyte DNA damage measured in groups of normal, healthy human volunteers. We applied the assay in investigations of human disease and occupational exposure of factory workers. (C) 1997 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalEnvironmental and Molecular Mutagenesis
Volume30
Issue number2
Publication statusPublished - 1997

Keywords

  • DNA damage
  • DNA repair
  • comet assay
  • human biomonitoring
  • diabetes
  • oxidative DNA-damage
  • lymphocytes
  • cancer
  • cells

Cite this

Collins, A., Dusinska, M., Franklin, M., Somorovska, M., Petrovska, H., Duthie, S., ... Vaughan, N. (1997). Comet assay in human biomonitoring studies: Reliability, validation, and applications. Environmental and Molecular Mutagenesis, 30(2), 139-146.