Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon

Sarah Maria Schmidt, Joanna Lukasiewicz, Rhys Farrer, Peter Dam, Chiara Bertoldo, Martijn Rep (Corresponding Author)

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Summary Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called ?avirulence? proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics-based genome comparison and genetic transformation of the fungus to identify AVRFOM2, the gene that encodes the avirulence protein recognized by the melon Fom-2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom-2-harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi. The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom-2-containing cultivars in the field.
Original languageEnglish
Pages (from-to)307-318
Number of pages12
JournalNew Phytologist
Volume209
Issue number1
Early online date25 Aug 2015
DOIs
Publication statusPublished - Jan 2016

Fingerprint

Fusarium oxysporum f. sp. melonis
Cucurbitaceae
Fusarium
Genomics
melons
genomics
Genes
Proteins
Fungi
genes
proteins
fungi
cultivars
Genome
genetic complementation
Genetic Transformation
Plant Diseases
Disease Resistance
genome
pathogens

Keywords

  • virulence gene
  • AVRFOM2
  • comparative genomics
  • Fusarium oxysporum f. sp. Melonis
  • gene-for-gene interaction
  • melon Fom-2 resistance gene

Cite this

Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon. / Schmidt, Sarah Maria; Lukasiewicz, Joanna; Farrer, Rhys; Dam, Peter; Bertoldo, Chiara; Rep, Martijn (Corresponding Author).

In: New Phytologist, Vol. 209, No. 1, 01.2016, p. 307-318.

Research output: Contribution to journalArticle

Schmidt, Sarah Maria ; Lukasiewicz, Joanna ; Farrer, Rhys ; Dam, Peter ; Bertoldo, Chiara ; Rep, Martijn. / Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon. In: New Phytologist. 2016 ; Vol. 209, No. 1. pp. 307-318.
@article{e324361721fd4a80814fa1bf194ac37d,
title = "Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon",
abstract = "Summary Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called ?avirulence? proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics-based genome comparison and genetic transformation of the fungus to identify AVRFOM2, the gene that encodes the avirulence protein recognized by the melon Fom-2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom-2-harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi. The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom-2-containing cultivars in the field.",
keywords = "virulence gene, AVRFOM2, comparative genomics, Fusarium oxysporum f. sp. Melonis, gene-for-gene interaction, melon Fom-2 resistance gene",
author = "Schmidt, {Sarah Maria} and Joanna Lukasiewicz and Rhys Farrer and Peter Dam and Chiara Bertoldo and Martijn Rep",
note = "We thank ENZA Zaden for the kind gift of melon Cha‐T and Cha‐Fom2 seeds, Thomas R. Gordon for the kind gift of the Fom VCG tester strains, Michel Pitrat for the kind gift of the Fom012, Fom013 and Fom016 strains, Rafi Perl‐Treves for the kind gift of the Fom009, Fom010 and Fom011 strains and Jose Alvarez Alvarez for the kind gift of Fom004, Fom005 and Fom006. We are grateful to Floris Stevens and Ian Ching for excellent technical help. This work was supported by the Innovational Research Incentives Scheme Vici of The Netherlands Organisation for Scientific Research (NWO) and the Horizon programme of the Netherlands Genomics Initiative through grants to M.R.",
year = "2016",
month = "1",
doi = "10.1111/nph.13584",
language = "English",
volume = "209",
pages = "307--318",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley/Blackwell (10.1111)",
number = "1",

}

TY - JOUR

T1 - Comparative genomics of Fusarium oxysporum f. sp. melonis reveals the secreted protein recognized by the Fom-2 resistance gene in melon

AU - Schmidt, Sarah Maria

AU - Lukasiewicz, Joanna

AU - Farrer, Rhys

AU - Dam, Peter

AU - Bertoldo, Chiara

AU - Rep, Martijn

N1 - We thank ENZA Zaden for the kind gift of melon Cha‐T and Cha‐Fom2 seeds, Thomas R. Gordon for the kind gift of the Fom VCG tester strains, Michel Pitrat for the kind gift of the Fom012, Fom013 and Fom016 strains, Rafi Perl‐Treves for the kind gift of the Fom009, Fom010 and Fom011 strains and Jose Alvarez Alvarez for the kind gift of Fom004, Fom005 and Fom006. We are grateful to Floris Stevens and Ian Ching for excellent technical help. This work was supported by the Innovational Research Incentives Scheme Vici of The Netherlands Organisation for Scientific Research (NWO) and the Horizon programme of the Netherlands Genomics Initiative through grants to M.R.

PY - 2016/1

Y1 - 2016/1

N2 - Summary Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called ?avirulence? proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics-based genome comparison and genetic transformation of the fungus to identify AVRFOM2, the gene that encodes the avirulence protein recognized by the melon Fom-2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom-2-harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi. The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom-2-containing cultivars in the field.

AB - Summary Development of resistant crops is the most effective way to control plant diseases to safeguard food and feed production. Disease resistance is commonly based on resistance genes, which generally mediate the recognition of small proteins secreted by invading pathogens. These proteins secreted by pathogens are called ?avirulence? proteins. Their identification is important for being able to assess the usefulness and durability of resistance genes in agricultural settings. We have used genome sequencing of a set of strains of the melon wilt fungus Fusarium oxysporum f. sp. melonis (Fom), bioinformatics-based genome comparison and genetic transformation of the fungus to identify AVRFOM2, the gene that encodes the avirulence protein recognized by the melon Fom-2 gene. Both an unbiased and a candidate gene approach identified a single candidate for the AVRFOM2 gene. Genetic complementation of AVRFOM2 in three different race 2 isolates resulted in resistance of Fom-2-harbouring melon cultivars. AvrFom2 is a small, secreted protein with two cysteine residues and weak similarity to secreted proteins of other fungi. The identification of AVRFOM2 will not only be helpful to select melon cultivars to avoid melon Fusarium wilt, but also to monitor how quickly a Fom population can adapt to deployment of Fom-2-containing cultivars in the field.

KW - virulence gene

KW - AVRFOM2

KW - comparative genomics

KW - Fusarium oxysporum f. sp. Melonis

KW - gene-for-gene interaction

KW - melon Fom-2 resistance gene

U2 - 10.1111/nph.13584

DO - 10.1111/nph.13584

M3 - Article

VL - 209

SP - 307

EP - 318

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -