Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae

Z Fuller, C A Maltin, Eric Milne, G S Mollison, J E Cox, C M Argo

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Evaluations of the energy requirements of working animals have been confounded by the constraints of indirect calorimetric techniques (Cal). This study sought to investigate a non-restrictive methodology, the doubly labelled water (DLW; (H2O)-H-2-O-18) technique, for the measurement of energy expenditure in free ranging equids. Six pony geldings were intravenously injected with DLW in two sequential studies that permitted first, isotope equilibration and half-lives to be determined and then second, heat production ( HP) to be measured simultaneously by DLW and traditional ( Cal) techniques.

    In study 1, three animals were injected with DLW, three animals were untreated controls. Blood samples were collected every 30 min for 12 h and thereafter at 24 h intervals for 14 days. Isotopes equilibrated throughout the body water pool within 300 (H-2) and 240 (O-18) min and half-lives were 6.3 +/- 0.6 days (H-2) and 5.6 +/- 0.4 days (O-18).

    In study 2, HP was simultaneously determined by Cal and DLW over a 4-day period. Animals (no. = 6) were assigned to pairs and in successive weeks two ponies were injected with DLW and confined to metabolism chambers 12 h later. Cal HP was 0.51 +/- 0.02 MJ/kg M-0.75 per day compared with 0.48 +/- 0.29 MJ/kg M 0.75 per day estimated by DLW. Maintenance metabolizable energy intake was 0.53 +/- 0.01 MJ/kg M 0.75 per day (Cal) and 0.50 +/- 0.01 MJ/kg M-0.75 per day (DLW). Validation of the DLW technique may empower essential, systematic appraisal of energy requirements in unrestrained working horses.

    Original languageEnglish
    Pages (from-to)293-303
    Number of pages11
    JournalAnimal Science
    Volume78
    Publication statusPublished - Apr 2004

    Keywords

    • deuterium
    • horses
    • metabolism
    • nutrition
    • oxygen-18
    • estimating heat-production
    • metabolic-rate
    • CO2 production
    • light horses
    • body water
    • ponies
    • vaidation
    • diets
    • animals
    • sheep

    Cite this

    Fuller, Z., Maltin, C. A., Milne, E., Mollison, G. S., Cox, J. E., & Argo, C. M. (2004). Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae. Animal Science, 78, 293-303.

    Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae. / Fuller, Z ; Maltin, C A ; Milne, Eric; Mollison, G S ; Cox, J E ; Argo, C M .

    In: Animal Science, Vol. 78, 04.2004, p. 293-303.

    Research output: Contribution to journalArticle

    Fuller, Z, Maltin, CA, Milne, E, Mollison, GS, Cox, JE & Argo, CM 2004, 'Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae', Animal Science, vol. 78, pp. 293-303.
    Fuller, Z ; Maltin, C A ; Milne, Eric ; Mollison, G S ; Cox, J E ; Argo, C M . / Comparison of calorimetry and the doubly labelled water technique for the measurement of energy expenditure in Equidae. In: Animal Science. 2004 ; Vol. 78. pp. 293-303.
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    AU - Maltin, C A

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    AU - Cox, J E

    AU - Argo, C M

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    AB - Evaluations of the energy requirements of working animals have been confounded by the constraints of indirect calorimetric techniques (Cal). This study sought to investigate a non-restrictive methodology, the doubly labelled water (DLW; (H2O)-H-2-O-18) technique, for the measurement of energy expenditure in free ranging equids. Six pony geldings were intravenously injected with DLW in two sequential studies that permitted first, isotope equilibration and half-lives to be determined and then second, heat production ( HP) to be measured simultaneously by DLW and traditional ( Cal) techniques.In study 1, three animals were injected with DLW, three animals were untreated controls. Blood samples were collected every 30 min for 12 h and thereafter at 24 h intervals for 14 days. Isotopes equilibrated throughout the body water pool within 300 (H-2) and 240 (O-18) min and half-lives were 6.3 +/- 0.6 days (H-2) and 5.6 +/- 0.4 days (O-18).In study 2, HP was simultaneously determined by Cal and DLW over a 4-day period. Animals (no. = 6) were assigned to pairs and in successive weeks two ponies were injected with DLW and confined to metabolism chambers 12 h later. Cal HP was 0.51 +/- 0.02 MJ/kg M-0.75 per day compared with 0.48 +/- 0.29 MJ/kg M 0.75 per day estimated by DLW. Maintenance metabolizable energy intake was 0.53 +/- 0.01 MJ/kg M 0.75 per day (Cal) and 0.50 +/- 0.01 MJ/kg M-0.75 per day (DLW). Validation of the DLW technique may empower essential, systematic appraisal of energy requirements in unrestrained working horses.

    KW - deuterium

    KW - horses

    KW - metabolism

    KW - nutrition

    KW - oxygen-18

    KW - estimating heat-production

    KW - metabolic-rate

    KW - CO2 production

    KW - light horses

    KW - body water

    KW - ponies

    KW - vaidation

    KW - diets

    KW - animals

    KW - sheep

    M3 - Article

    VL - 78

    SP - 293

    EP - 303

    JO - Animal Science

    JF - Animal Science

    SN - 1357-7298

    ER -