Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes

F Canavez, Neil Thomas Young, L A Guethlein, R Rajalingam, S I Khakoo, B P Shum, P Parham

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The leukocyte receptor complex (LRC) on human chromosome 19 contains related Ig superfamily killer cell Ig-like receptor (KIR) and leukocyte Ig-like receptor (LIR) genes. Previously, we discovered much difference in the KIR genes between humans and chimpanzees, primate species estimated to have approximately 98.8% genomic sequence similarity. Here, the common chimpanzee LIR genes are identified, characterized, and compared with their human counterparts. From screening a chimpanzee splenocyte cDNA library, clones corresponding to nine different chimpanzee LIRs were isolated and sequenced. Analysis of genomic DNA from 48 unrelated chimpanzees showed 42 to have all nine LIR genes, and six animals to lack just one of the genes. In structural diversity and functional type, the chimpanzee LIRs cover the range of human LIRs. Although both species have the same number of inhibitory LIRs, humans have more activating receptors, a trend also seen for KIRs. Four chimpanzee LIRs are clearly orthologs of human LIRs. Five other chimpanzee LIRs have paralogous relationships with clusters of human LIRs and have undergone much recombination. Like the human genes, chimpanzee LIR genes appear to be organized into two duplicated blocks, each block containing two orthologous genes. This organization provides a conserved framework within which there are clusters of faster evolving genes. Human and chimpanzee KIR genes have an analogous arrangement. Whereas both KIR and LIR genes can exhibit greater interspecies differences than the genome average, within each species the LIR gene family is more conserved than the KIR gene family.
Original languageEnglish
Pages (from-to)5786-5794
Number of pages9
JournalThe Journal of Immunology
Volume167
Issue number10
Publication statusPublished - 15 Nov 2001

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Pan troglodytes
Leukocytes
Genes
Chromosomes, Human, Pair 19
Human Chromosomes
Gene Library
Primates
Genetic Recombination
Clone Cells

Keywords

  • Animals
  • Cloning, Molecular
  • Conserved Sequence
  • Evolution, Molecular
  • Haplotypes
  • Humans
  • Multigene Family
  • Pan troglodytes
  • Phylogeny
  • Receptors, Immunologic
  • Receptors, KIR
  • Recombination, Genetic
  • Sequence Homology, Amino Acid

Cite this

Canavez, F., Young, N. T., Guethlein, L. A., Rajalingam, R., Khakoo, S. I., Shum, B. P., & Parham, P. (2001). Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes. The Journal of Immunology, 167(10), 5786-5794.

Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes. / Canavez, F; Young, Neil Thomas; Guethlein, L A; Rajalingam, R; Khakoo, S I; Shum, B P; Parham, P.

In: The Journal of Immunology, Vol. 167, No. 10, 15.11.2001, p. 5786-5794.

Research output: Contribution to journalArticle

Canavez, F, Young, NT, Guethlein, LA, Rajalingam, R, Khakoo, SI, Shum, BP & Parham, P 2001, 'Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes', The Journal of Immunology, vol. 167, no. 10, pp. 5786-5794.
Canavez F, Young NT, Guethlein LA, Rajalingam R, Khakoo SI, Shum BP et al. Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes. The Journal of Immunology. 2001 Nov 15;167(10):5786-5794.
Canavez, F ; Young, Neil Thomas ; Guethlein, L A ; Rajalingam, R ; Khakoo, S I ; Shum, B P ; Parham, P. / Comparison of chimpanzee and human leukocyte Ig-like receptor genes reveals framework and rapidly evolving genes. In: The Journal of Immunology. 2001 ; Vol. 167, No. 10. pp. 5786-5794.
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abstract = "The leukocyte receptor complex (LRC) on human chromosome 19 contains related Ig superfamily killer cell Ig-like receptor (KIR) and leukocyte Ig-like receptor (LIR) genes. Previously, we discovered much difference in the KIR genes between humans and chimpanzees, primate species estimated to have approximately 98.8{\%} genomic sequence similarity. Here, the common chimpanzee LIR genes are identified, characterized, and compared with their human counterparts. From screening a chimpanzee splenocyte cDNA library, clones corresponding to nine different chimpanzee LIRs were isolated and sequenced. Analysis of genomic DNA from 48 unrelated chimpanzees showed 42 to have all nine LIR genes, and six animals to lack just one of the genes. In structural diversity and functional type, the chimpanzee LIRs cover the range of human LIRs. Although both species have the same number of inhibitory LIRs, humans have more activating receptors, a trend also seen for KIRs. Four chimpanzee LIRs are clearly orthologs of human LIRs. Five other chimpanzee LIRs have paralogous relationships with clusters of human LIRs and have undergone much recombination. Like the human genes, chimpanzee LIR genes appear to be organized into two duplicated blocks, each block containing two orthologous genes. This organization provides a conserved framework within which there are clusters of faster evolving genes. Human and chimpanzee KIR genes have an analogous arrangement. Whereas both KIR and LIR genes can exhibit greater interspecies differences than the genome average, within each species the LIR gene family is more conserved than the KIR gene family.",
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AB - The leukocyte receptor complex (LRC) on human chromosome 19 contains related Ig superfamily killer cell Ig-like receptor (KIR) and leukocyte Ig-like receptor (LIR) genes. Previously, we discovered much difference in the KIR genes between humans and chimpanzees, primate species estimated to have approximately 98.8% genomic sequence similarity. Here, the common chimpanzee LIR genes are identified, characterized, and compared with their human counterparts. From screening a chimpanzee splenocyte cDNA library, clones corresponding to nine different chimpanzee LIRs were isolated and sequenced. Analysis of genomic DNA from 48 unrelated chimpanzees showed 42 to have all nine LIR genes, and six animals to lack just one of the genes. In structural diversity and functional type, the chimpanzee LIRs cover the range of human LIRs. Although both species have the same number of inhibitory LIRs, humans have more activating receptors, a trend also seen for KIRs. Four chimpanzee LIRs are clearly orthologs of human LIRs. Five other chimpanzee LIRs have paralogous relationships with clusters of human LIRs and have undergone much recombination. Like the human genes, chimpanzee LIR genes appear to be organized into two duplicated blocks, each block containing two orthologous genes. This organization provides a conserved framework within which there are clusters of faster evolving genes. Human and chimpanzee KIR genes have an analogous arrangement. Whereas both KIR and LIR genes can exhibit greater interspecies differences than the genome average, within each species the LIR gene family is more conserved than the KIR gene family.

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KW - Haplotypes

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KW - Pan troglodytes

KW - Phylogeny

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KW - Sequence Homology, Amino Acid

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