Complementary use of molecular and element-specific mass spectrometry for identification of selenium compounds related to human selenium metabolism

Bente Gammelgaard, Charlotte Gabel-Jensen, Stefan Sturup, Helle Ruesz Hansen

Research output: Contribution to journalLiterature review

55 Citations (Scopus)

Abstract

The aim of this paper is to give an overview of analytical data on the identification of selenium compounds in biological samples with relevance for selenium metabolism. Only studies applying the combination of element-specific inductively coupled plasma mass spectrometry as well as molecular electrospray mass spectrometry detection have been included. Hence, selenium compounds are only considered identified if molecular mass spectra obtained by analysis of the authentic biological sample have been provided. Selenium compounds identified in selenium-accumulating plants and yeast are included, as extracts from such plants and yeast have been widely used for examination of the cancer-preventive effect of selenium in cell lines, animal models and human intervention trials. Hence, these selenium compounds are available for absorption and further metabolism. Identification of selenium metabolites in simulated gastric and intestinal juice, intestinal epithelial tissue, liver and urine is described. Hence, selenium metabolites identified in relation to absorption, metabolism and excretion are included.

Original languageEnglish
Pages (from-to)1691-1706
Number of pages16
JournalAnalytical and Bioanalytical Chemistry
Volume390
Issue number7
Early online date6 Jan 2008
DOIs
Publication statusPublished - Apr 2008

Keywords

  • bioanalytical methods
  • biological samples
  • high-performance liquid chromatography
  • mass spectrometry
  • inductively coupled mass spectrometry
  • speciation
  • inductively-coupled plasma
  • HPLC-ICP-MS
  • performance liquid-chromatography
  • glutamyl-se-methylselenocysteine
  • garlic allium-sativum
  • ion-pairing agents
  • OF-FLIGHT-MS/MS
  • human urine
  • speciation analysis
  • in-vitro

Cite this