Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space

E. J. Bateman, Elizabeth Baggs

Research output: Contribution to journalArticle

557 Citations (Scopus)

Abstract

A combination of stable isotope and acetylene (0.01% v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha(-1)) silt loam soil at contrasting (20-70%) water-filled pore space (WFPS). N-15-N2O emissions from (NH4NO3)-N-14-N-15 replicates were attributed to denitrification and N-15-N2O from (NH4NO3)-N-15-N-15 minus that from (NH4NO3)-N-14-N-15 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of N-15-NO3-. All of the N2O emitted at 70% WFPS (31.6 mg N2O-N m(-2) over 24 days; 1.12 mu g N2O-N g dry soil(-1); 0.16% of N applied) was produced during denitrification, but at 35-60% WFPS nitrification was the main process producing N2O, accounting for 81% of N-15-N2O emitted at 60% WFPS, and 7.9 mu g N-15-N2O m(-2) (0.28 ng N-15-N2O g dry soil(-1)) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50% WFPS treatment and accounted for 20% of N-15-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20% WFPS (2.6 mu g N-15-N2O m(-2) over 7 days; 0.09 ng N-15-N2O g dry soil(-1); 85% of (15)pN-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.

Original languageEnglish
Pages (from-to)379-388
Number of pages9
JournalBiology and Fertility of Soils
Volume41
DOIs
Publication statusPublished - 2005

Keywords

  • denitrification
  • nitrification
  • nitrous oxide
  • soil water-filled pore space
  • NITROUS-OXIDE EMISSIONS
  • HETEROTROPHIC NITRIFICATION
  • NITRIFIER DENITRIFICATION
  • NITROSOMONAS-EUROPAEA
  • ALCALIGENES-FAECALIS
  • NITRIFYING BACTERIA
  • AGRICULTURAL SOILS
  • AGGREGATED SOILS
  • POOL DILUTION
  • NO

Cite this

Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. / Bateman, E. J.; Baggs, Elizabeth.

In: Biology and Fertility of Soils, Vol. 41, 2005, p. 379-388.

Research output: Contribution to journalArticle

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abstract = "A combination of stable isotope and acetylene (0.01{\%} v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha(-1)) silt loam soil at contrasting (20-70{\%}) water-filled pore space (WFPS). N-15-N2O emissions from (NH4NO3)-N-14-N-15 replicates were attributed to denitrification and N-15-N2O from (NH4NO3)-N-15-N-15 minus that from (NH4NO3)-N-14-N-15 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of N-15-NO3-. All of the N2O emitted at 70{\%} WFPS (31.6 mg N2O-N m(-2) over 24 days; 1.12 mu g N2O-N g dry soil(-1); 0.16{\%} of N applied) was produced during denitrification, but at 35-60{\%} WFPS nitrification was the main process producing N2O, accounting for 81{\%} of N-15-N2O emitted at 60{\%} WFPS, and 7.9 mu g N-15-N2O m(-2) (0.28 ng N-15-N2O g dry soil(-1)) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50{\%} WFPS treatment and accounted for 20{\%} of N-15-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20{\%} WFPS (2.6 mu g N-15-N2O m(-2) over 7 days; 0.09 ng N-15-N2O g dry soil(-1); 85{\%} of (15)pN-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.",
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T1 - Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space

AU - Bateman, E. J.

AU - Baggs, Elizabeth

PY - 2005

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N2 - A combination of stable isotope and acetylene (0.01% v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha(-1)) silt loam soil at contrasting (20-70%) water-filled pore space (WFPS). N-15-N2O emissions from (NH4NO3)-N-14-N-15 replicates were attributed to denitrification and N-15-N2O from (NH4NO3)-N-15-N-15 minus that from (NH4NO3)-N-14-N-15 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of N-15-NO3-. All of the N2O emitted at 70% WFPS (31.6 mg N2O-N m(-2) over 24 days; 1.12 mu g N2O-N g dry soil(-1); 0.16% of N applied) was produced during denitrification, but at 35-60% WFPS nitrification was the main process producing N2O, accounting for 81% of N-15-N2O emitted at 60% WFPS, and 7.9 mu g N-15-N2O m(-2) (0.28 ng N-15-N2O g dry soil(-1)) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50% WFPS treatment and accounted for 20% of N-15-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20% WFPS (2.6 mu g N-15-N2O m(-2) over 7 days; 0.09 ng N-15-N2O g dry soil(-1); 85% of (15)pN-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.

AB - A combination of stable isotope and acetylene (0.01% v/v) inhibition techniques were used for the first time to determine N2O production during denitrification, autotrophic nitrification and heterotrophic nitrification in a fertilised (200 kg N ha(-1)) silt loam soil at contrasting (20-70%) water-filled pore space (WFPS). N-15-N2O emissions from (NH4NO3)-N-14-N-15 replicates were attributed to denitrification and N-15-N2O from (NH4NO3)-N-15-N-15 minus that from (NH4NO3)-N-14-N-15 replicates was attributed to nitrification and heterotrophic nitrification in the presence of acetylene, as there was no dissimilatory nitrate reduction to ammonium or immobilisation and remineralisation of N-15-NO3-. All of the N2O emitted at 70% WFPS (31.6 mg N2O-N m(-2) over 24 days; 1.12 mu g N2O-N g dry soil(-1); 0.16% of N applied) was produced during denitrification, but at 35-60% WFPS nitrification was the main process producing N2O, accounting for 81% of N-15-N2O emitted at 60% WFPS, and 7.9 mu g N-15-N2O m(-2) (0.28 ng N-15-N2O g dry soil(-1)) was estimated to be emitted over 7 days during heterotrophic nitrification in the 50% WFPS treatment and accounted for 20% of N-15-N2O from this treatment. Denitrification was the predominant N2O-producing process at 20% WFPS (2.6 mu g N-15-N2O m(-2) over 7 days; 0.09 ng N-15-N2O g dry soil(-1); 85% of (15)pN-N2O from this treatment) and may have been due to the occurrence of aerobic denitrification at this WFPS. Our results demonstrate the usefulness of a combined stable isotope and acetylene approach to quantify N2O emissions from different processes and to show that several processes may contribute to N2O emission from agricultural soils depending on soil WFPS.

KW - denitrification

KW - nitrification

KW - nitrous oxide

KW - soil water-filled pore space

KW - NITROUS-OXIDE EMISSIONS

KW - HETEROTROPHIC NITRIFICATION

KW - NITRIFIER DENITRIFICATION

KW - NITROSOMONAS-EUROPAEA

KW - ALCALIGENES-FAECALIS

KW - NITRIFYING BACTERIA

KW - AGRICULTURAL SOILS

KW - AGGREGATED SOILS

KW - POOL DILUTION

KW - NO

U2 - 10.1007/s00374-005-0858-3

DO - 10.1007/s00374-005-0858-3

M3 - Article

VL - 41

SP - 379

EP - 388

JO - Biology and Fertility of Soils

JF - Biology and Fertility of Soils

SN - 0178-2762

ER -