Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer

A Preliminary Clinical Study

Gabriella Baio, Giuseppe Rescinito, Francesca Rosa, Daniele Pace, Simona Boccardo, Luca Basso, Sandra Salvi, Massimo Calabrese, Mauro Truini, Carlo Emanuele Neumaier

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

PURPOSE: The calcium-sensing receptor (CaSR) is overexpressed in many pathological states including breast cancer. Since choline kinase may be activated in breast cancer cells by CaSR resulting in increased phosphocholine production, we sought to correlate the total choline peak in breast lesions as measured by in vivo proton magnetic resonance spectroscopy ((1)H-MRS) with the CaSR expression levels in surgical specimens.

PROCEDURES: Thirty-six patients with breast lesions were MR scanned at 3T scanner. Tumour morphology and DCE-MR kinetics were evaluated. (1)H-MRS was applied for Cho detection and compared with the CaSR immunohistochemistry analysis (score 0-5) on surgical breast specimens.

RESULTS: Thirty-four lesions demonstrated a DCE malignant kinetics curve (types 2 and 3), while two lesions showed a benign (type 1). Twenty of the 23 breast cancer lesions (87 %) with a consistent Cho peak expressed a CaSR score of 3-5, and ten of the 11 breast lesions negative for Cho (91 %) had a CaSR score of 1-2. The two benign lesions showed a non-uniform/weak intense expression of the CaSR (score 3) with a consistent Cho peak.

CONCLUSIONS: The presence or absence of choline peak evaluated by (1)H-MRS, well correlated with the expression of CaSR in patients with breast lesions (p < 0.01), supports the hypothesis that CaSR may play an important role in the production of choline in breast cancer.

Original languageEnglish
Pages (from-to)548-556
Number of pages9
JournalMolecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging
Volume17
Issue number4
Early online date23 Jan 2015
DOIs
Publication statusPublished - Aug 2015

Fingerprint

Calcium-Sensing Receptors
Choline
Magnetic Resonance Spectroscopy
Breast Neoplasms
Breast
Choline Kinase
Clinical Studies
Phosphorylcholine
Immunohistochemistry

Keywords

  • calcium-sensing receptor
  • MR spectroscopy
  • breast cancer
  • phosphocholine
  • 3T MR
  • magnetic resonance imaging
  • G protein-coupled receptor
  • Calcium channels
  • MRI breast
  • Molecular imaging cancer

Cite this

Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer : A Preliminary Clinical Study. / Baio, Gabriella; Rescinito, Giuseppe; Rosa, Francesca; Pace, Daniele; Boccardo, Simona; Basso, Luca; Salvi, Sandra; Calabrese, Massimo; Truini, Mauro; Neumaier, Carlo Emanuele.

In: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 17, No. 4, 08.2015, p. 548-556.

Research output: Contribution to journalArticle

Baio, Gabriella ; Rescinito, Giuseppe ; Rosa, Francesca ; Pace, Daniele ; Boccardo, Simona ; Basso, Luca ; Salvi, Sandra ; Calabrese, Massimo ; Truini, Mauro ; Neumaier, Carlo Emanuele. / Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer : A Preliminary Clinical Study. In: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 2015 ; Vol. 17, No. 4. pp. 548-556.
@article{22e4222ca7334c01ad575b0e07609cab,
title = "Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study",
abstract = "PURPOSE: The calcium-sensing receptor (CaSR) is overexpressed in many pathological states including breast cancer. Since choline kinase may be activated in breast cancer cells by CaSR resulting in increased phosphocholine production, we sought to correlate the total choline peak in breast lesions as measured by in vivo proton magnetic resonance spectroscopy ((1)H-MRS) with the CaSR expression levels in surgical specimens.PROCEDURES: Thirty-six patients with breast lesions were MR scanned at 3T scanner. Tumour morphology and DCE-MR kinetics were evaluated. (1)H-MRS was applied for Cho detection and compared with the CaSR immunohistochemistry analysis (score 0-5) on surgical breast specimens.RESULTS: Thirty-four lesions demonstrated a DCE malignant kinetics curve (types 2 and 3), while two lesions showed a benign (type 1). Twenty of the 23 breast cancer lesions (87 {\%}) with a consistent Cho peak expressed a CaSR score of 3-5, and ten of the 11 breast lesions negative for Cho (91 {\%}) had a CaSR score of 1-2. The two benign lesions showed a non-uniform/weak intense expression of the CaSR (score 3) with a consistent Cho peak.CONCLUSIONS: The presence or absence of choline peak evaluated by (1)H-MRS, well correlated with the expression of CaSR in patients with breast lesions (p < 0.01), supports the hypothesis that CaSR may play an important role in the production of choline in breast cancer.",
keywords = "calcium-sensing receptor, MR spectroscopy, breast cancer, phosphocholine, 3T MR, magnetic resonance imaging, G protein-coupled receptor, Calcium channels , MRI breast, Molecular imaging cancer",
author = "Gabriella Baio and Giuseppe Rescinito and Francesca Rosa and Daniele Pace and Simona Boccardo and Luca Basso and Sandra Salvi and Massimo Calabrese and Mauro Truini and Neumaier, {Carlo Emanuele}",
note = "Acknowledgments. The authors thank Mrs Teresa Morris, Coordinator of Aberdeen Biomedical Imaging, University of Aberdeen, UK, for his helpful revision of the manuscript, in particular for English editing",
year = "2015",
month = "8",
doi = "10.1007/s11307-015-0823-y",
language = "English",
volume = "17",
pages = "548--556",
journal = "Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging",
issn = "1860-2002",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer

T2 - A Preliminary Clinical Study

AU - Baio, Gabriella

AU - Rescinito, Giuseppe

AU - Rosa, Francesca

AU - Pace, Daniele

AU - Boccardo, Simona

AU - Basso, Luca

AU - Salvi, Sandra

AU - Calabrese, Massimo

AU - Truini, Mauro

AU - Neumaier, Carlo Emanuele

N1 - Acknowledgments. The authors thank Mrs Teresa Morris, Coordinator of Aberdeen Biomedical Imaging, University of Aberdeen, UK, for his helpful revision of the manuscript, in particular for English editing

PY - 2015/8

Y1 - 2015/8

N2 - PURPOSE: The calcium-sensing receptor (CaSR) is overexpressed in many pathological states including breast cancer. Since choline kinase may be activated in breast cancer cells by CaSR resulting in increased phosphocholine production, we sought to correlate the total choline peak in breast lesions as measured by in vivo proton magnetic resonance spectroscopy ((1)H-MRS) with the CaSR expression levels in surgical specimens.PROCEDURES: Thirty-six patients with breast lesions were MR scanned at 3T scanner. Tumour morphology and DCE-MR kinetics were evaluated. (1)H-MRS was applied for Cho detection and compared with the CaSR immunohistochemistry analysis (score 0-5) on surgical breast specimens.RESULTS: Thirty-four lesions demonstrated a DCE malignant kinetics curve (types 2 and 3), while two lesions showed a benign (type 1). Twenty of the 23 breast cancer lesions (87 %) with a consistent Cho peak expressed a CaSR score of 3-5, and ten of the 11 breast lesions negative for Cho (91 %) had a CaSR score of 1-2. The two benign lesions showed a non-uniform/weak intense expression of the CaSR (score 3) with a consistent Cho peak.CONCLUSIONS: The presence or absence of choline peak evaluated by (1)H-MRS, well correlated with the expression of CaSR in patients with breast lesions (p < 0.01), supports the hypothesis that CaSR may play an important role in the production of choline in breast cancer.

AB - PURPOSE: The calcium-sensing receptor (CaSR) is overexpressed in many pathological states including breast cancer. Since choline kinase may be activated in breast cancer cells by CaSR resulting in increased phosphocholine production, we sought to correlate the total choline peak in breast lesions as measured by in vivo proton magnetic resonance spectroscopy ((1)H-MRS) with the CaSR expression levels in surgical specimens.PROCEDURES: Thirty-six patients with breast lesions were MR scanned at 3T scanner. Tumour morphology and DCE-MR kinetics were evaluated. (1)H-MRS was applied for Cho detection and compared with the CaSR immunohistochemistry analysis (score 0-5) on surgical breast specimens.RESULTS: Thirty-four lesions demonstrated a DCE malignant kinetics curve (types 2 and 3), while two lesions showed a benign (type 1). Twenty of the 23 breast cancer lesions (87 %) with a consistent Cho peak expressed a CaSR score of 3-5, and ten of the 11 breast lesions negative for Cho (91 %) had a CaSR score of 1-2. The two benign lesions showed a non-uniform/weak intense expression of the CaSR (score 3) with a consistent Cho peak.CONCLUSIONS: The presence or absence of choline peak evaluated by (1)H-MRS, well correlated with the expression of CaSR in patients with breast lesions (p < 0.01), supports the hypothesis that CaSR may play an important role in the production of choline in breast cancer.

KW - calcium-sensing receptor

KW - MR spectroscopy

KW - breast cancer

KW - phosphocholine

KW - 3T MR

KW - magnetic resonance imaging

KW - G protein-coupled receptor

KW - Calcium channels

KW - MRI breast

KW - Molecular imaging cancer

U2 - 10.1007/s11307-015-0823-y

DO - 10.1007/s11307-015-0823-y

M3 - Article

VL - 17

SP - 548

EP - 556

JO - Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging

JF - Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging

SN - 1860-2002

IS - 4

ER -