Abstract
Background
Cysteamine has recently been shown to have in vitro properties potentially therapeutically beneficial in cystic fibrosis (CF). In this study we investigated the antimicrobial and mucolytic activity of cysteamine against the complex biologic matrix of CF sputum.
Methods
Sputum samples were obtained from 23 CF adults. Sputum polymicrobial content after in vitro exposure to cysteamine and standard CF antibiotics was assessed after a single exposure and after 14 days low-dose exposure. The effect of cysteamine on sputum spinnbarkeit was assessed.
Findings
Cysteamine reduced sputum polymicrobial burden by 3 · 18 (95% CI 2 · 30–4 · 07, p < 0.001) log10 units after 24 h incubation. Combined cysteamine and tobramycin reduced polymicrobial burden by a further 3 · 75 (95% CI 2 · 63–5 · 07, p < 0 · 001) log10 units above that seen with tobramycin. Repeated low dosing with cysteamine reduced sputum polymicrobial load from day 10 onwards (p = 0.032). Cysteamine reduced CF sputum viscoelasticity, sputum spinnbarkeit cysteamine 11.1 mm/s (95% CI 3.95–18.2) vs DNAse 1.69 mm/s (95% CI 0.73–2.65), p = 0.016. Cysteamine was active against Mycobacterium abscessus as a monotherapy and also potentiated the effects of amikacin and azithromycin.
Original language | English |
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Pages (from-to) | 1507-1512 |
Number of pages | 6 |
Journal | EBioMedicine |
Volume | 2 |
Issue number | 10 |
Early online date | 10 Aug 2015 |
DOIs | |
Publication status | Published - Oct 2015 |
Bibliographical note
Date of Acceptance: 07/08/2015Acknowledgement
This study was funded by Scottish Enterprise Encompass Kick Start Award KSB001.
Keywords
- cysteamine
- cystic fibrosis
- mycobacterium abscessus
- pseudomonas aeruginosa
- sputum