Cytokine gene polymorphism in sympathetic ophthalmia

D. Atan, S. J. Turner, D. J. Kilmartin, John Vincent Forrester, J. Bidwell, A. D. Dick, A. J. Churchill

Research output: Contribution to journalArticle

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Abstract

PURPOSE. Sympathetic ophthalmia ( SO) is a prototypical autoimmune disease in which injury to one eye causes sight-threatening inflammation in the otherwise normal contralateral eye. Previous work found that human leukocyte antigen alleles HLA-DRB1*04 and DQA1*03 are markers of increased susceptibility and severity in British and Irish patients. Evidence is accumulating that single nucleotide polymorphisms ( SNPs) in cytokine genes can also influence the development of autoimmune disease through their effect on levels of cytokine production. The purpose of this study was to determine whether polymorphisms in the cytokine genes are important markers for disease severity and outcome in patients with SO.

METHODS. Twenty-six British and Irish patients meeting well-efined criteria for the diagnosis of SO were compared with 48 matched controls. Genotyping of SNPs in the TNF alpha, TNF beta, and IL-10 genes was performed using the polymerase chain reaction and sequence-specific primers (SSP-PCRs) and of the CTLA-4 and TNF receptor 2 genes using restriction length polymorphism-PCR (RFLP-PCR).

RESULTS. Significant associations were found between the IL-10 - 1082 SNP and disease recurrence from previously stable disease and the level of steroids required for maintenance therapy. In addition, the GCC IL-10 promoter haplotype ( IL-10 - 1082G, - 819C, - 592C) was found to be protective against disease recurrence.

CONCLUSIONS. These results show that cytokine gene polymorphisms are markers for the severity of disease in SO. They were found to be associated with recurrence of previously stable disease and with the level of maintenance steroid treatment required to control inflammatory activity.

Original languageEnglish
Pages (from-to)4245-4250
Number of pages5
JournalInvestigative Ophthalmology & Visual Science
Volume46
DOIs
Publication statusPublished - 2005

Keywords

  • TUMOR-NECROSIS-FACTOR
  • EXPERIMENTAL AUTOIMMUNE UVEORETINITIS
  • MAJOR HISTOCOMPATIBILITY COMPLEX
  • CD4(+) T-CELLS
  • SYSTEMIC-LUPUS-ERYTHEMATOSUS
  • RETINAL GLIAL-CELLS
  • TNF-ALPHA
  • RHEUMATOID-ARTHRITIS
  • GRAVES-DISEASE
  • DEFICIENT MICE

Cite this

Atan, D., Turner, S. J., Kilmartin, D. J., Forrester, J. V., Bidwell, J., Dick, A. D., & Churchill, A. J. (2005). Cytokine gene polymorphism in sympathetic ophthalmia. Investigative Ophthalmology & Visual Science, 46, 4245-4250. https://doi.org/10.1167/iovs.05-0126

Cytokine gene polymorphism in sympathetic ophthalmia. / Atan, D.; Turner, S. J.; Kilmartin, D. J.; Forrester, John Vincent; Bidwell, J.; Dick, A. D.; Churchill, A. J.

In: Investigative Ophthalmology & Visual Science, Vol. 46, 2005, p. 4245-4250.

Research output: Contribution to journalArticle

Atan, D, Turner, SJ, Kilmartin, DJ, Forrester, JV, Bidwell, J, Dick, AD & Churchill, AJ 2005, 'Cytokine gene polymorphism in sympathetic ophthalmia', Investigative Ophthalmology & Visual Science, vol. 46, pp. 4245-4250. https://doi.org/10.1167/iovs.05-0126
Atan D, Turner SJ, Kilmartin DJ, Forrester JV, Bidwell J, Dick AD et al. Cytokine gene polymorphism in sympathetic ophthalmia. Investigative Ophthalmology & Visual Science. 2005;46:4245-4250. https://doi.org/10.1167/iovs.05-0126
Atan, D. ; Turner, S. J. ; Kilmartin, D. J. ; Forrester, John Vincent ; Bidwell, J. ; Dick, A. D. ; Churchill, A. J. / Cytokine gene polymorphism in sympathetic ophthalmia. In: Investigative Ophthalmology & Visual Science. 2005 ; Vol. 46. pp. 4245-4250.
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abstract = "PURPOSE. Sympathetic ophthalmia ( SO) is a prototypical autoimmune disease in which injury to one eye causes sight-threatening inflammation in the otherwise normal contralateral eye. Previous work found that human leukocyte antigen alleles HLA-DRB1*04 and DQA1*03 are markers of increased susceptibility and severity in British and Irish patients. Evidence is accumulating that single nucleotide polymorphisms ( SNPs) in cytokine genes can also influence the development of autoimmune disease through their effect on levels of cytokine production. The purpose of this study was to determine whether polymorphisms in the cytokine genes are important markers for disease severity and outcome in patients with SO.METHODS. Twenty-six British and Irish patients meeting well-efined criteria for the diagnosis of SO were compared with 48 matched controls. Genotyping of SNPs in the TNF alpha, TNF beta, and IL-10 genes was performed using the polymerase chain reaction and sequence-specific primers (SSP-PCRs) and of the CTLA-4 and TNF receptor 2 genes using restriction length polymorphism-PCR (RFLP-PCR).RESULTS. Significant associations were found between the IL-10 - 1082 SNP and disease recurrence from previously stable disease and the level of steroids required for maintenance therapy. In addition, the GCC IL-10 promoter haplotype ( IL-10 - 1082G, - 819C, - 592C) was found to be protective against disease recurrence.CONCLUSIONS. These results show that cytokine gene polymorphisms are markers for the severity of disease in SO. They were found to be associated with recurrence of previously stable disease and with the level of maintenance steroid treatment required to control inflammatory activity.",
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T1 - Cytokine gene polymorphism in sympathetic ophthalmia

AU - Atan, D.

AU - Turner, S. J.

AU - Kilmartin, D. J.

AU - Forrester, John Vincent

AU - Bidwell, J.

AU - Dick, A. D.

AU - Churchill, A. J.

PY - 2005

Y1 - 2005

N2 - PURPOSE. Sympathetic ophthalmia ( SO) is a prototypical autoimmune disease in which injury to one eye causes sight-threatening inflammation in the otherwise normal contralateral eye. Previous work found that human leukocyte antigen alleles HLA-DRB1*04 and DQA1*03 are markers of increased susceptibility and severity in British and Irish patients. Evidence is accumulating that single nucleotide polymorphisms ( SNPs) in cytokine genes can also influence the development of autoimmune disease through their effect on levels of cytokine production. The purpose of this study was to determine whether polymorphisms in the cytokine genes are important markers for disease severity and outcome in patients with SO.METHODS. Twenty-six British and Irish patients meeting well-efined criteria for the diagnosis of SO were compared with 48 matched controls. Genotyping of SNPs in the TNF alpha, TNF beta, and IL-10 genes was performed using the polymerase chain reaction and sequence-specific primers (SSP-PCRs) and of the CTLA-4 and TNF receptor 2 genes using restriction length polymorphism-PCR (RFLP-PCR).RESULTS. Significant associations were found between the IL-10 - 1082 SNP and disease recurrence from previously stable disease and the level of steroids required for maintenance therapy. In addition, the GCC IL-10 promoter haplotype ( IL-10 - 1082G, - 819C, - 592C) was found to be protective against disease recurrence.CONCLUSIONS. These results show that cytokine gene polymorphisms are markers for the severity of disease in SO. They were found to be associated with recurrence of previously stable disease and with the level of maintenance steroid treatment required to control inflammatory activity.

AB - PURPOSE. Sympathetic ophthalmia ( SO) is a prototypical autoimmune disease in which injury to one eye causes sight-threatening inflammation in the otherwise normal contralateral eye. Previous work found that human leukocyte antigen alleles HLA-DRB1*04 and DQA1*03 are markers of increased susceptibility and severity in British and Irish patients. Evidence is accumulating that single nucleotide polymorphisms ( SNPs) in cytokine genes can also influence the development of autoimmune disease through their effect on levels of cytokine production. The purpose of this study was to determine whether polymorphisms in the cytokine genes are important markers for disease severity and outcome in patients with SO.METHODS. Twenty-six British and Irish patients meeting well-efined criteria for the diagnosis of SO were compared with 48 matched controls. Genotyping of SNPs in the TNF alpha, TNF beta, and IL-10 genes was performed using the polymerase chain reaction and sequence-specific primers (SSP-PCRs) and of the CTLA-4 and TNF receptor 2 genes using restriction length polymorphism-PCR (RFLP-PCR).RESULTS. Significant associations were found between the IL-10 - 1082 SNP and disease recurrence from previously stable disease and the level of steroids required for maintenance therapy. In addition, the GCC IL-10 promoter haplotype ( IL-10 - 1082G, - 819C, - 592C) was found to be protective against disease recurrence.CONCLUSIONS. These results show that cytokine gene polymorphisms are markers for the severity of disease in SO. They were found to be associated with recurrence of previously stable disease and with the level of maintenance steroid treatment required to control inflammatory activity.

KW - TUMOR-NECROSIS-FACTOR

KW - EXPERIMENTAL AUTOIMMUNE UVEORETINITIS

KW - MAJOR HISTOCOMPATIBILITY COMPLEX

KW - CD4(+) T-CELLS

KW - SYSTEMIC-LUPUS-ERYTHEMATOSUS

KW - RETINAL GLIAL-CELLS

KW - TNF-ALPHA

KW - RHEUMATOID-ARTHRITIS

KW - GRAVES-DISEASE

KW - DEFICIENT MICE

U2 - 10.1167/iovs.05-0126

DO - 10.1167/iovs.05-0126

M3 - Article

VL - 46

SP - 4245

EP - 4250

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 0146-0404

ER -