Degradation of specific polyunsaturated fatty acids in red blood cells stored at -20 degrees C proceeds faster in patients with schizophrenia when compared with healthy controls

Helen Catherine Fox, B. M. Ross, D. Horrobin, I. Glen, D. Tocher, David Malcolm St Clair

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Deficits in red blood cell (RBC) polyunsaturated fatty acids (PUFAs) have been extensively reported in schizophrenia although reports are inconsistent. A possible explanation for this inconsistency is varying storage conditions of blood samples prior to analysis, especially freezer storage temperature. We conducted a prospective investigation of fatty acid degradation rates in RBCs from healthy control subjects when samples from each individual were stored at both -20degreesC or -70degreesC. Differences were detected between storage conditions. A second prospective study was conducted to investigate the effect of differential storage conditions on RBC membrane fatty acids from schizophrenic patients. We found that storage at -20degreesC was associated with reduced levels of PUFAs. Comparison of decay rates suggest that schizophrenics decay approximately twice as rapidly as controls. Furthermore, this phenomenon appears to be specific for the longer chain PUFAs suggesting that an enzymatic process may be responsible, e.g. elevated phospholipase A(2) activity, as opposed to simple chemical oxidation. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)291-297
Number of pages6
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume69
Issue number5
DOIs
Publication statusPublished - 2003

Keywords

  • PLASMA-LIPID PEROXIDE
  • VITAMIN-E LEVELS
  • TARDIVE-DYSKINESIA
  • PHOSPHOLIPASE-A2
  • BRAIN

Cite this

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title = "Degradation of specific polyunsaturated fatty acids in red blood cells stored at -20 degrees C proceeds faster in patients with schizophrenia when compared with healthy controls",
abstract = "Deficits in red blood cell (RBC) polyunsaturated fatty acids (PUFAs) have been extensively reported in schizophrenia although reports are inconsistent. A possible explanation for this inconsistency is varying storage conditions of blood samples prior to analysis, especially freezer storage temperature. We conducted a prospective investigation of fatty acid degradation rates in RBCs from healthy control subjects when samples from each individual were stored at both -20degreesC or -70degreesC. Differences were detected between storage conditions. A second prospective study was conducted to investigate the effect of differential storage conditions on RBC membrane fatty acids from schizophrenic patients. We found that storage at -20degreesC was associated with reduced levels of PUFAs. Comparison of decay rates suggest that schizophrenics decay approximately twice as rapidly as controls. Furthermore, this phenomenon appears to be specific for the longer chain PUFAs suggesting that an enzymatic process may be responsible, e.g. elevated phospholipase A(2) activity, as opposed to simple chemical oxidation. (C) 2003 Elsevier Ltd. All rights reserved.",
keywords = "PLASMA-LIPID PEROXIDE, VITAMIN-E LEVELS, TARDIVE-DYSKINESIA, PHOSPHOLIPASE-A2, BRAIN",
author = "Fox, {Helen Catherine} and Ross, {B. M.} and D. Horrobin and I. Glen and D. Tocher and {St Clair}, {David Malcolm}",
year = "2003",
doi = "10.1016/S0952-3278(03)00084-X",
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pages = "291--297",
journal = "Prostaglandins, Leukotrienes and Essential Fatty Acids",
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T1 - Degradation of specific polyunsaturated fatty acids in red blood cells stored at -20 degrees C proceeds faster in patients with schizophrenia when compared with healthy controls

AU - Fox, Helen Catherine

AU - Ross, B. M.

AU - Horrobin, D.

AU - Glen, I.

AU - Tocher, D.

AU - St Clair, David Malcolm

PY - 2003

Y1 - 2003

N2 - Deficits in red blood cell (RBC) polyunsaturated fatty acids (PUFAs) have been extensively reported in schizophrenia although reports are inconsistent. A possible explanation for this inconsistency is varying storage conditions of blood samples prior to analysis, especially freezer storage temperature. We conducted a prospective investigation of fatty acid degradation rates in RBCs from healthy control subjects when samples from each individual were stored at both -20degreesC or -70degreesC. Differences were detected between storage conditions. A second prospective study was conducted to investigate the effect of differential storage conditions on RBC membrane fatty acids from schizophrenic patients. We found that storage at -20degreesC was associated with reduced levels of PUFAs. Comparison of decay rates suggest that schizophrenics decay approximately twice as rapidly as controls. Furthermore, this phenomenon appears to be specific for the longer chain PUFAs suggesting that an enzymatic process may be responsible, e.g. elevated phospholipase A(2) activity, as opposed to simple chemical oxidation. (C) 2003 Elsevier Ltd. All rights reserved.

AB - Deficits in red blood cell (RBC) polyunsaturated fatty acids (PUFAs) have been extensively reported in schizophrenia although reports are inconsistent. A possible explanation for this inconsistency is varying storage conditions of blood samples prior to analysis, especially freezer storage temperature. We conducted a prospective investigation of fatty acid degradation rates in RBCs from healthy control subjects when samples from each individual were stored at both -20degreesC or -70degreesC. Differences were detected between storage conditions. A second prospective study was conducted to investigate the effect of differential storage conditions on RBC membrane fatty acids from schizophrenic patients. We found that storage at -20degreesC was associated with reduced levels of PUFAs. Comparison of decay rates suggest that schizophrenics decay approximately twice as rapidly as controls. Furthermore, this phenomenon appears to be specific for the longer chain PUFAs suggesting that an enzymatic process may be responsible, e.g. elevated phospholipase A(2) activity, as opposed to simple chemical oxidation. (C) 2003 Elsevier Ltd. All rights reserved.

KW - PLASMA-LIPID PEROXIDE

KW - VITAMIN-E LEVELS

KW - TARDIVE-DYSKINESIA

KW - PHOSPHOLIPASE-A2

KW - BRAIN

U2 - 10.1016/S0952-3278(03)00084-X

DO - 10.1016/S0952-3278(03)00084-X

M3 - Article

VL - 69

SP - 291

EP - 297

JO - Prostaglandins, Leukotrienes and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 5

ER -