Design of Maleimide: Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

Emma J. Wright, Maciej Sosna, Sally Bloodworth*, Jeremy D. Kilburn, Philip N. Bartlett

*Corresponding author for this work

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (approximate to 7.5pmolcm(-2)).

    Original languageEnglish
    Pages (from-to)5550-5554
    Number of pages5
    JournalChemistry : a European Journal
    Volume20
    Issue number19
    Early online date2 May 2014
    DOIs
    Publication statusPublished - 5 May 2014

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