Design of Maleimide: Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

Emma J. Wright; Maciej Sosna; Sally Bloodworth; Jeremy D. Kilburn; Philip N. Bartlett

doi:10.1002/chem.201400246

Design of Maleimide: Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

Emma J. Wright, Maciej Sosna, Sally Bloodworth^*, Jeremy D. Kilburn, Philip N. Bartlett

^*Corresponding author for this work

University of Southampton

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (approximate to 7.5pmolcm(-2)).

Original language	English
Pages (from-to)	5550-5554
Number of pages	5
Journal	Chemistry : a European Journal
Volume	20
Issue number	19
Early online date	2 May 2014
DOIs	https://doi.org/10.1002/chem.201400246
Publication status	Published - 5 May 2014

Bibliographical note

Acknowledgements
We thank H. Hamzah for running background voltammograms on the unmodified glassy carbon electrodes. This work was funded by the European Research Council, project 3D-Nano-BioDevice NMP4-SL-2009–22925.

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abstract = "Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (approximate to 7.5pmolcm(-2)).",

author = "Wright, {Emma J.} and Maciej Sosna and Sally Bloodworth and Kilburn, {Jeremy D.} and Bartlett, {Philip N.}",

note = "Acknowledgements We thank H. Hamzah for running background voltammograms on the unmodified glassy carbon electrodes. This work was funded by the European Research Council, project 3D-Nano-BioDevice NMP4-SL-2009–22925.",

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T2 - Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

AU - Wright, Emma J.

AU - Sosna, Maciej

AU - Bloodworth, Sally

AU - Kilburn, Jeremy D.

AU - Bartlett, Philip N.

N1 - Acknowledgements We thank H. Hamzah for running background voltammograms on the unmodified glassy carbon electrodes. This work was funded by the European Research Council, project 3D-Nano-BioDevice NMP4-SL-2009–22925.

PY - 2014/5/5

Y1 - 2014/5/5

N2 - Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (approximate to 7.5pmolcm(-2)).

AB - Mixed two-component monolayers on glassy carbon are prepared by electrochemical oxidation of N-(2-aminoethyl)acetamide and mono-N-Boc-hexamethylenediamine in mixed solution. Subsequent N-deprotection, amide coupling and solid-phase synthetic steps lead to electrode-surface functionalisation with maleimide, with controlled partial coverage of this cysteine-binding group at appropriate dilution for covalent immobilisation of a model redox-active protein, cytochrome c, with high coverage (approximate to 7.5pmolcm(-2)).

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DO - 10.1002/chem.201400246

M3 - Article

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JO - Chemistry : a European Journal

JF - Chemistry : a European Journal

IS - 19

ER -

Design of Maleimide: Functionalised Electrodes for Covalent Attachment of Proteins through Free Surface Cysteine Groups

Abstract

Bibliographical note

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