Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments: A set of behaviors that are relevant for bioremediation

Rungroch Sungthong, Pieter Van West, Manuel Cantos, Jose Julio Ortega-Calvo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, we assessed the development (formation, taxis and settlement) of eukaryotic zoospores under different regimes of exposure to polycyclic aromatic hydrocarbons (PAHs), which imitated environmental scenarios of pollution and bioremediation. With this aim, we used an oomycete, Pythium aphanidermatum, as a source of zoospores and two PAH-degrading bacteria (Mycobacterium gilvum VM552 and Pseudomonas putida G7). The oomycete and both bacteria were not antagonistic, and zoospore formation was diminished only in the presence of the highest bacterial cell density (108–1010 colony-forming units mL− 1). A negative influence of PAHs on zoospore formation and taxis was observed when PAHs were exposed in combination with organic solutions and polar solvents. Co-exposure of PAHs with non-polar solvents [hexadecane (HD) and 2,2,4,4,6,8,8-heptamethylnonane (HMN)] did not affect zoospore settlement at the interfaces of the organic solvents and water. However, zoospores settled and created mycelial networks only at HD–water interfaces. Both bacteria diminished the toxic influence of PAHs on zoospore formation and taxis, and they did not interrupt zoospore settlement. The results suggest that zoospore development could be applicable for toxicity assessment of PAHs and enhancement of their bioavailability. Microbial interactions during both swimming modes and community formation at pollutant interfaces were revealed as major factors that have potential relevance to bioremediation.
Original languageEnglish
Pages (from-to)767-776
Number of pages10
JournalScience of the Total Environment
Volume511
Early online date22 Jan 2015
DOIs
Publication statusPublished - 1 Apr 2015

Fingerprint

Bioremediation
Polycyclic Aromatic Hydrocarbons
Polycyclic aromatic hydrocarbons
bioremediation
PAH
taxis
Bacteria
bacterium
Poisons
Organic solvents
bioavailability
Toxicity
Pollution
toxicity
pollution
pollutant
Water

Keywords

  • eukaryotic zoospore
  • oomycetes
  • PAH-polluted scenario
  • PAH-degrading bacteria
  • zoospore development
  • bioremediation

Cite this

Development of eukaryotic zoospores within polycyclic aromatic hydrocarbon (PAH)-polluted environments : A set of behaviors that are relevant for bioremediation. / Sungthong, Rungroch ; Van West, Pieter; Cantos, Manuel; Ortega-Calvo, Jose Julio .

In: Science of the Total Environment, Vol. 511, 01.04.2015, p. 767-776.

Research output: Contribution to journalArticle

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abstract = "In this study, we assessed the development (formation, taxis and settlement) of eukaryotic zoospores under different regimes of exposure to polycyclic aromatic hydrocarbons (PAHs), which imitated environmental scenarios of pollution and bioremediation. With this aim, we used an oomycete, Pythium aphanidermatum, as a source of zoospores and two PAH-degrading bacteria (Mycobacterium gilvum VM552 and Pseudomonas putida G7). The oomycete and both bacteria were not antagonistic, and zoospore formation was diminished only in the presence of the highest bacterial cell density (108–1010 colony-forming units mL− 1). A negative influence of PAHs on zoospore formation and taxis was observed when PAHs were exposed in combination with organic solutions and polar solvents. Co-exposure of PAHs with non-polar solvents [hexadecane (HD) and 2,2,4,4,6,8,8-heptamethylnonane (HMN)] did not affect zoospore settlement at the interfaces of the organic solvents and water. However, zoospores settled and created mycelial networks only at HD–water interfaces. Both bacteria diminished the toxic influence of PAHs on zoospore formation and taxis, and they did not interrupt zoospore settlement. The results suggest that zoospore development could be applicable for toxicity assessment of PAHs and enhancement of their bioavailability. Microbial interactions during both swimming modes and community formation at pollutant interfaces were revealed as major factors that have potential relevance to bioremediation.",
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