Dietary methylmercury alters the proteome in Atlantic salmon (Salmo salar) kidney

O J Nøstbakken, S A M Martin, B E Torstensen, H Amlund, P Cash, P A Olsvik

Research output: Contribution to journalArticle

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Abstract

Background
Methylmercury (MeHg) is an environmental contaminant most known for its severe neurotoxic effects. Although accumulation of MeHg tends to be several folds higher in kidney compared to other tissues, studies on nephrotoxic effects are almost non-existing. In this study we aim to investigate the toxicity of dietary MeHg in kidney of Atlantic salmon (Salmo salar).

Material and methods
Atlantic salmon were exposed to dietary MeHg for a period of 8 weeks, before the fish were euthanized and kidney was sampled for proteomic and real time RT-PCR analysis, as well as for mercury determination. Protein separation was done with 2-D PAGE, and differentially regulated spots were picked for analysis using liquid chromatography MS/MS analysis. Moreover, whole blood and liver tissue were sampled for mercury determination and real time RT-PCR (liver).

Results
MeHg exposed fish accumulated significantly more mercury (Hg) than control fish. The proteomic analysis revealed differential abundance of 26 spots in the kidney, and 14 of these protein spots were successfully identified. The proteins identified indicated effects of MeHg on; metabolism, inflammation, oxidative stress, protein-folding, and cell-structural components. Gene expression analysis of selected markers revealed few differentially regulated transcripts in kidney and liver in the exposed fish compared to the control fish. However, the affected transcripts indicated a disruption in the expression of two metabolic markers due to MeHg exposure in liver.

Conclusion
This study suggests that dietary MeHg has similar effects in kidney as previously shown for other tissues in fish. The effects observed were in markers for oxidative stress, inflammation and energy metabolism. The identification of proteomic markers in this study provides a basis for a better understanding of MeHg-induced nephrotoxicity in fish.
Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalAquatic Toxicology
Volume108
Early online date27 Aug 2011
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Salmo salar
methylmercury compounds
methylmercury
Proteome
proteome
Fishes
kidneys
Kidney
fish
proteomics
Mercury
mercury
Proteomics
liver
protein
Liver
Real-Time Polymerase Chain Reaction
Oxidative Stress
oxidative stress
inflammation

Keywords

  • methylmercury
  • kidney
  • Atlantic salmon
  • proteomics

Cite this

Dietary methylmercury alters the proteome in Atlantic salmon (Salmo salar) kidney. / Nøstbakken, O J; Martin, S A M; Torstensen, B E; Amlund, H; Cash, P; Olsvik, P A.

In: Aquatic Toxicology, Vol. 108, 02.2012, p. 70-77.

Research output: Contribution to journalArticle

Nøstbakken, O J ; Martin, S A M ; Torstensen, B E ; Amlund, H ; Cash, P ; Olsvik, P A. / Dietary methylmercury alters the proteome in Atlantic salmon (Salmo salar) kidney. In: Aquatic Toxicology. 2012 ; Vol. 108. pp. 70-77.
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abstract = "BackgroundMethylmercury (MeHg) is an environmental contaminant most known for its severe neurotoxic effects. Although accumulation of MeHg tends to be several folds higher in kidney compared to other tissues, studies on nephrotoxic effects are almost non-existing. In this study we aim to investigate the toxicity of dietary MeHg in kidney of Atlantic salmon (Salmo salar).Material and methodsAtlantic salmon were exposed to dietary MeHg for a period of 8 weeks, before the fish were euthanized and kidney was sampled for proteomic and real time RT-PCR analysis, as well as for mercury determination. Protein separation was done with 2-D PAGE, and differentially regulated spots were picked for analysis using liquid chromatography MS/MS analysis. Moreover, whole blood and liver tissue were sampled for mercury determination and real time RT-PCR (liver).ResultsMeHg exposed fish accumulated significantly more mercury (Hg) than control fish. The proteomic analysis revealed differential abundance of 26 spots in the kidney, and 14 of these protein spots were successfully identified. The proteins identified indicated effects of MeHg on; metabolism, inflammation, oxidative stress, protein-folding, and cell-structural components. Gene expression analysis of selected markers revealed few differentially regulated transcripts in kidney and liver in the exposed fish compared to the control fish. However, the affected transcripts indicated a disruption in the expression of two metabolic markers due to MeHg exposure in liver.ConclusionThis study suggests that dietary MeHg has similar effects in kidney as previously shown for other tissues in fish. The effects observed were in markers for oxidative stress, inflammation and energy metabolism. The identification of proteomic markers in this study provides a basis for a better understanding of MeHg-induced nephrotoxicity in fish.",
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AU - Martin, S A M

AU - Torstensen, B E

AU - Amlund, H

AU - Cash, P

AU - Olsvik, P A

N1 - Copyright 2011 Elsevier B.V., All rights reserved.

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N2 - BackgroundMethylmercury (MeHg) is an environmental contaminant most known for its severe neurotoxic effects. Although accumulation of MeHg tends to be several folds higher in kidney compared to other tissues, studies on nephrotoxic effects are almost non-existing. In this study we aim to investigate the toxicity of dietary MeHg in kidney of Atlantic salmon (Salmo salar).Material and methodsAtlantic salmon were exposed to dietary MeHg for a period of 8 weeks, before the fish were euthanized and kidney was sampled for proteomic and real time RT-PCR analysis, as well as for mercury determination. Protein separation was done with 2-D PAGE, and differentially regulated spots were picked for analysis using liquid chromatography MS/MS analysis. Moreover, whole blood and liver tissue were sampled for mercury determination and real time RT-PCR (liver).ResultsMeHg exposed fish accumulated significantly more mercury (Hg) than control fish. The proteomic analysis revealed differential abundance of 26 spots in the kidney, and 14 of these protein spots were successfully identified. The proteins identified indicated effects of MeHg on; metabolism, inflammation, oxidative stress, protein-folding, and cell-structural components. Gene expression analysis of selected markers revealed few differentially regulated transcripts in kidney and liver in the exposed fish compared to the control fish. However, the affected transcripts indicated a disruption in the expression of two metabolic markers due to MeHg exposure in liver.ConclusionThis study suggests that dietary MeHg has similar effects in kidney as previously shown for other tissues in fish. The effects observed were in markers for oxidative stress, inflammation and energy metabolism. The identification of proteomic markers in this study provides a basis for a better understanding of MeHg-induced nephrotoxicity in fish.

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