Dietary vitamin E decreases ESR signal intensity in hepatic microsomal preparations from malignant hyperthermia susceptible pigs

Garry G. Duthie, Donald B. McPhail, Philip C. Morrice, John R. Arthur

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

On incubation with the spin trap α-(4-pyridyl-l-oxide)-N-tert-butylnitrone (4-POBN), a characteristic electron spin resonance (ESR) signal was produced at a greater rate in hepatic microsomal fractions from malignant hyprthermia susceptible (MHS) pigs compared with resistant (MHR) pigs. This was accompanied by increased formation of thiobarbituric acid reactive substances (TBARS). Supplementation of diets for six weeks with 235 mg α-tocopherol acetate/kg significantly increased microsomal vitamin E content of both pig types. Moreover, the rate of formation of TBARS and ESR signal height of incubated microsomes from supplemented MHS pigs was decreased to that of MHR pigs. Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were also moderated by dietary vitamin E. Vitamin E supplementation may decrease the peroxidative events associated with MH.
Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalFree Radical Research Communications
Volume14
Issue number2
DOIs
Publication statusPublished - 1991

Keywords

  • malignant hyperthermia
  • vitamin-E
  • electron spin resonance
  • lipid peroxidation
  • performance
  • cells

Cite this

@article{db279c734a294d8ab08367696b10295a,
title = "Dietary vitamin E decreases ESR signal intensity in hepatic microsomal preparations from malignant hyperthermia susceptible pigs",
abstract = "On incubation with the spin trap α-(4-pyridyl-l-oxide)-N-tert-butylnitrone (4-POBN), a characteristic electron spin resonance (ESR) signal was produced at a greater rate in hepatic microsomal fractions from malignant hyprthermia susceptible (MHS) pigs compared with resistant (MHR) pigs. This was accompanied by increased formation of thiobarbituric acid reactive substances (TBARS). Supplementation of diets for six weeks with 235 mg α-tocopherol acetate/kg significantly increased microsomal vitamin E content of both pig types. Moreover, the rate of formation of TBARS and ESR signal height of incubated microsomes from supplemented MHS pigs was decreased to that of MHR pigs. Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were also moderated by dietary vitamin E. Vitamin E supplementation may decrease the peroxidative events associated with MH.",
keywords = "malignant hyperthermia, vitamin-E, electron spin resonance, lipid peroxidation, performance, cells",
author = "Duthie, {Garry G.} and McPhail, {Donald B.} and Morrice, {Philip C.} and Arthur, {John R.}",
year = "1991",
doi = "10.3109/10715769109094125",
language = "English",
volume = "14",
pages = "133--137",
journal = "Free Radical Research Communications",
issn = "8755-0199",
publisher = "Taylor and Francis Ltd.",
number = "2",

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TY - JOUR

T1 - Dietary vitamin E decreases ESR signal intensity in hepatic microsomal preparations from malignant hyperthermia susceptible pigs

AU - Duthie, Garry G.

AU - McPhail, Donald B.

AU - Morrice, Philip C.

AU - Arthur, John R.

PY - 1991

Y1 - 1991

N2 - On incubation with the spin trap α-(4-pyridyl-l-oxide)-N-tert-butylnitrone (4-POBN), a characteristic electron spin resonance (ESR) signal was produced at a greater rate in hepatic microsomal fractions from malignant hyprthermia susceptible (MHS) pigs compared with resistant (MHR) pigs. This was accompanied by increased formation of thiobarbituric acid reactive substances (TBARS). Supplementation of diets for six weeks with 235 mg α-tocopherol acetate/kg significantly increased microsomal vitamin E content of both pig types. Moreover, the rate of formation of TBARS and ESR signal height of incubated microsomes from supplemented MHS pigs was decreased to that of MHR pigs. Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were also moderated by dietary vitamin E. Vitamin E supplementation may decrease the peroxidative events associated with MH.

AB - On incubation with the spin trap α-(4-pyridyl-l-oxide)-N-tert-butylnitrone (4-POBN), a characteristic electron spin resonance (ESR) signal was produced at a greater rate in hepatic microsomal fractions from malignant hyprthermia susceptible (MHS) pigs compared with resistant (MHR) pigs. This was accompanied by increased formation of thiobarbituric acid reactive substances (TBARS). Supplementation of diets for six weeks with 235 mg α-tocopherol acetate/kg significantly increased microsomal vitamin E content of both pig types. Moreover, the rate of formation of TBARS and ESR signal height of incubated microsomes from supplemented MHS pigs was decreased to that of MHR pigs. Elevated pyruvate kinase activities and TBARS concentrations in plasma of MHS pigs were also moderated by dietary vitamin E. Vitamin E supplementation may decrease the peroxidative events associated with MH.

KW - malignant hyperthermia

KW - vitamin-E

KW - electron spin resonance

KW - lipid peroxidation

KW - performance

KW - cells

U2 - 10.3109/10715769109094125

DO - 10.3109/10715769109094125

M3 - Article

VL - 14

SP - 133

EP - 137

JO - Free Radical Research Communications

JF - Free Radical Research Communications

SN - 8755-0199

IS - 2

ER -