Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding

Erik Keimpema, Klaudia Barabas, Yury M Morozov, Giuseppe Tortoriello, Masaaki Torii, Gary Cameron, Yuchio Yanagawa, Masahiko Watanabe, Ken Mackie, Tibor Harkany

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Abstract

Endocannabinoids, particularly 2-arachidonoyl glycerol (2-AG), impact the directional turning and motility of a developing axon by activating CB(1) cannabinoid receptors (CB(1)Rs) in its growth cone. Recent findings posit that sn-1-diacylglycerol lipases (DAGLa/ß) synthesize 2-AG in the motile axon segment of developing pyramidal cells. Coincident axonal targeting of CB(1)Rs and DAGLs prompts the hypothesis that autocrine 2-AG signaling facilitates axonal outgrowth. However, DAGLs alone are insufficient to account for the spatial specificity and dynamics of 2-AG signaling. Therefore, we hypothesized that local 2-AG degradation by monoacylglycerol lipase (MGL) must play a role. We determined how subcellular recruitment of MGL is temporally and spatially restricted to establish the signaling competence of 2-AG during axonal growth. MGL is expressed in central and peripheral axons of the fetal nervous system by embryonic day 12.5. MGL coexists with DAGLa and CB(1)Rs in corticofugal axons of pyramidal cells. Here, MGL and DAGLa undergo differential axonal targeting with MGL being excluded from the motile neurite tip. Thus, spatially confined MGL activity generates a 2-AG-sensing microdomain and configures 2-AG signaling to promote axonal growth. Once synaptogenesis commences, MGL disperses in stationary growth cones. The axonal polarity of MGL is maintained by differential proteasomal degradation because inhibiting the ubiquitin proteasome system also induces axonal MGL redistribution. Because MGL inactivation drives a CB(1)R-dependent axonal growth response, we conclude that 2-AG may act as a focal protrusive signal for developing neurons and whose regulated metabolism is critical for attaining correct axonal complexity.
Original languageEnglish
Pages (from-to)13992-14007
Number of pages16
JournalJournal of Neuroscience
Volume30
Issue number42
DOIs
Publication statusPublished - 20 Oct 2010

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Monoacylglycerol Lipases
Cannabinoid Receptors
Axons
Growth Cones
Pyramidal Cells
Axon Guidance
2-arachidonylglycerol
Growth
Endocannabinoids
Lipoprotein Lipase
Neurites
Proteasome Endopeptidase Complex
Ubiquitin
Mental Competency
Nervous System

Keywords

  • animals
  • arachidonic acids
  • axons
  • blotting, western
  • cells, cultured
  • chromatography, high pressure liquid
  • endocannabinoids
  • glutamate decarboxylase
  • glycerides
  • immunohistochemistry
  • lipoprotein lipase
  • mice
  • mice, inbred C57BL
  • microscopy, electron
  • monoacylglycerol lipases
  • neural pathways
  • neurons
  • pyramidal cells
  • receptor, cannabinoid, CB1
  • reverse transcriptase polymerase chain reaction
  • signal transduction
  • subcellular fractions
  • tandem mass spectrometry

Cite this

Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding. / Keimpema, Erik; Barabas, Klaudia; Morozov, Yury M; Tortoriello, Giuseppe; Torii, Masaaki; Cameron, Gary; Yanagawa, Yuchio; Watanabe, Masahiko; Mackie, Ken; Harkany, Tibor.

In: Journal of Neuroscience, Vol. 30, No. 42, 20.10.2010, p. 13992-14007.

Research output: Contribution to journalArticle

Keimpema, E, Barabas, K, Morozov, YM, Tortoriello, G, Torii, M, Cameron, G, Yanagawa, Y, Watanabe, M, Mackie, K & Harkany, T 2010, 'Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding', Journal of Neuroscience, vol. 30, no. 42, pp. 13992-14007. https://doi.org/10.1523/JNEUROSCI.2126-10.2010
Keimpema, Erik ; Barabas, Klaudia ; Morozov, Yury M ; Tortoriello, Giuseppe ; Torii, Masaaki ; Cameron, Gary ; Yanagawa, Yuchio ; Watanabe, Masahiko ; Mackie, Ken ; Harkany, Tibor. / Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 42. pp. 13992-14007.
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AU - Keimpema, Erik

AU - Barabas, Klaudia

AU - Morozov, Yury M

AU - Tortoriello, Giuseppe

AU - Torii, Masaaki

AU - Cameron, Gary

AU - Yanagawa, Yuchio

AU - Watanabe, Masahiko

AU - Mackie, Ken

AU - Harkany, Tibor

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N2 - Endocannabinoids, particularly 2-arachidonoyl glycerol (2-AG), impact the directional turning and motility of a developing axon by activating CB(1) cannabinoid receptors (CB(1)Rs) in its growth cone. Recent findings posit that sn-1-diacylglycerol lipases (DAGLa/ß) synthesize 2-AG in the motile axon segment of developing pyramidal cells. Coincident axonal targeting of CB(1)Rs and DAGLs prompts the hypothesis that autocrine 2-AG signaling facilitates axonal outgrowth. However, DAGLs alone are insufficient to account for the spatial specificity and dynamics of 2-AG signaling. Therefore, we hypothesized that local 2-AG degradation by monoacylglycerol lipase (MGL) must play a role. We determined how subcellular recruitment of MGL is temporally and spatially restricted to establish the signaling competence of 2-AG during axonal growth. MGL is expressed in central and peripheral axons of the fetal nervous system by embryonic day 12.5. MGL coexists with DAGLa and CB(1)Rs in corticofugal axons of pyramidal cells. Here, MGL and DAGLa undergo differential axonal targeting with MGL being excluded from the motile neurite tip. Thus, spatially confined MGL activity generates a 2-AG-sensing microdomain and configures 2-AG signaling to promote axonal growth. Once synaptogenesis commences, MGL disperses in stationary growth cones. The axonal polarity of MGL is maintained by differential proteasomal degradation because inhibiting the ubiquitin proteasome system also induces axonal MGL redistribution. Because MGL inactivation drives a CB(1)R-dependent axonal growth response, we conclude that 2-AG may act as a focal protrusive signal for developing neurons and whose regulated metabolism is critical for attaining correct axonal complexity.

AB - Endocannabinoids, particularly 2-arachidonoyl glycerol (2-AG), impact the directional turning and motility of a developing axon by activating CB(1) cannabinoid receptors (CB(1)Rs) in its growth cone. Recent findings posit that sn-1-diacylglycerol lipases (DAGLa/ß) synthesize 2-AG in the motile axon segment of developing pyramidal cells. Coincident axonal targeting of CB(1)Rs and DAGLs prompts the hypothesis that autocrine 2-AG signaling facilitates axonal outgrowth. However, DAGLs alone are insufficient to account for the spatial specificity and dynamics of 2-AG signaling. Therefore, we hypothesized that local 2-AG degradation by monoacylglycerol lipase (MGL) must play a role. We determined how subcellular recruitment of MGL is temporally and spatially restricted to establish the signaling competence of 2-AG during axonal growth. MGL is expressed in central and peripheral axons of the fetal nervous system by embryonic day 12.5. MGL coexists with DAGLa and CB(1)Rs in corticofugal axons of pyramidal cells. Here, MGL and DAGLa undergo differential axonal targeting with MGL being excluded from the motile neurite tip. Thus, spatially confined MGL activity generates a 2-AG-sensing microdomain and configures 2-AG signaling to promote axonal growth. Once synaptogenesis commences, MGL disperses in stationary growth cones. The axonal polarity of MGL is maintained by differential proteasomal degradation because inhibiting the ubiquitin proteasome system also induces axonal MGL redistribution. Because MGL inactivation drives a CB(1)R-dependent axonal growth response, we conclude that 2-AG may act as a focal protrusive signal for developing neurons and whose regulated metabolism is critical for attaining correct axonal complexity.

KW - animals

KW - arachidonic acids

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KW - cells, cultured

KW - chromatography, high pressure liquid

KW - endocannabinoids

KW - glutamate decarboxylase

KW - glycerides

KW - immunohistochemistry

KW - lipoprotein lipase

KW - mice

KW - mice, inbred C57BL

KW - microscopy, electron

KW - monoacylglycerol lipases

KW - neural pathways

KW - neurons

KW - pyramidal cells

KW - receptor, cannabinoid, CB1

KW - reverse transcriptase polymerase chain reaction

KW - signal transduction

KW - subcellular fractions

KW - tandem mass spectrometry

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DO - 10.1523/JNEUROSCI.2126-10.2010

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VL - 30

SP - 13992

EP - 14007

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

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ER -