Differential translocation of rho family GTPases by lysophosphatidic acid, endothelin-1, and platelet-derived growth factor

I N Fleming, C M Elliott, J H Exton

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The small GTPases of the Rho family play a key role in a number of signaling pathways activated by lysophosphatidic acid (LPA). However, little is known concerning the mechanism of regulation of these proteins. In this study we demonstrate that in Swiss 3T3 fibroblasts, LPA induces a sustained, time-dependent relocalization of RhoA to the Triton X-100-soluble low speed membrane fraction, which can be reversed by removal of LPA from the medium. Translocation was only observed with micromolar concentrations of LPA and was inhibited by pretreating the cells with pertussis toxin but not with tyrosine kinase inhibitors. LPA also induced translocation of CDC42Hs to the membranes but had no effect on the distribution of Rac1, RhoB, or Rho-GDI. Translocation of RhoA was also induced by endothelin-1. Conversely, platelet-derived growth factor did not cause the translocation of RhoA to any membrane fraction but stimulated relocalization of Rac1 to the high speed membrane fraction. Significantly, incubation of cell lysates with guanosine 5'-O-(thiotriphosphate) was sufficient to translocate RhoA, Rac1, and CDC42Hs from the cytosol to the membranes, whereas incubation with GDP had the opposite effect. These data suggest that the translocation of the Rho family proteins to the membrane fraction is controlled by their activation state and that agonists show selectivity in inducing the activation/translocation of these proteins.
Original languageEnglish
Pages (from-to)33067-73
Number of pages7
JournalThe Journal of Biological Chemistry
Volume271
Issue number51
Publication statusPublished - 1996

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rho GTP-Binding Proteins
GTP Phosphohydrolases
Platelet-Derived Growth Factor
Endothelin-1
Membranes
rho-Specific Guanine Nucleotide Dissociation Inhibitors
Chemical activation
Monomeric GTP-Binding Proteins
Guanosine
Pertussis Toxin
Octoxynol
Protein Transport
Proteins
Cytosol
Protein-Tyrosine Kinases
Fibroblasts
Membrane Proteins
lysophosphatidic acid
Cells

Keywords

  • 3T3 Cells
  • Animals
  • Cell Compartmentation
  • Endothelin-1
  • GTP-Binding Proteins
  • GTPase-Activating Proteins
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Intracellular Membranes
  • Lysophospholipids
  • Mice
  • Nuclear Envelope
  • Pertussis Toxin
  • Platelet-Derived Growth Factor
  • Proteins
  • Virulence Factors, Bordetella
  • rhoA GTP-Binding Protein

Cite this

Differential translocation of rho family GTPases by lysophosphatidic acid, endothelin-1, and platelet-derived growth factor. / Fleming, I N; Elliott, C M; Exton, J H.

In: The Journal of Biological Chemistry, Vol. 271, No. 51, 1996, p. 33067-73.

Research output: Contribution to journalArticle

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AU - Exton, J H

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N2 - The small GTPases of the Rho family play a key role in a number of signaling pathways activated by lysophosphatidic acid (LPA). However, little is known concerning the mechanism of regulation of these proteins. In this study we demonstrate that in Swiss 3T3 fibroblasts, LPA induces a sustained, time-dependent relocalization of RhoA to the Triton X-100-soluble low speed membrane fraction, which can be reversed by removal of LPA from the medium. Translocation was only observed with micromolar concentrations of LPA and was inhibited by pretreating the cells with pertussis toxin but not with tyrosine kinase inhibitors. LPA also induced translocation of CDC42Hs to the membranes but had no effect on the distribution of Rac1, RhoB, or Rho-GDI. Translocation of RhoA was also induced by endothelin-1. Conversely, platelet-derived growth factor did not cause the translocation of RhoA to any membrane fraction but stimulated relocalization of Rac1 to the high speed membrane fraction. Significantly, incubation of cell lysates with guanosine 5'-O-(thiotriphosphate) was sufficient to translocate RhoA, Rac1, and CDC42Hs from the cytosol to the membranes, whereas incubation with GDP had the opposite effect. These data suggest that the translocation of the Rho family proteins to the membrane fraction is controlled by their activation state and that agonists show selectivity in inducing the activation/translocation of these proteins.

AB - The small GTPases of the Rho family play a key role in a number of signaling pathways activated by lysophosphatidic acid (LPA). However, little is known concerning the mechanism of regulation of these proteins. In this study we demonstrate that in Swiss 3T3 fibroblasts, LPA induces a sustained, time-dependent relocalization of RhoA to the Triton X-100-soluble low speed membrane fraction, which can be reversed by removal of LPA from the medium. Translocation was only observed with micromolar concentrations of LPA and was inhibited by pretreating the cells with pertussis toxin but not with tyrosine kinase inhibitors. LPA also induced translocation of CDC42Hs to the membranes but had no effect on the distribution of Rac1, RhoB, or Rho-GDI. Translocation of RhoA was also induced by endothelin-1. Conversely, platelet-derived growth factor did not cause the translocation of RhoA to any membrane fraction but stimulated relocalization of Rac1 to the high speed membrane fraction. Significantly, incubation of cell lysates with guanosine 5'-O-(thiotriphosphate) was sufficient to translocate RhoA, Rac1, and CDC42Hs from the cytosol to the membranes, whereas incubation with GDP had the opposite effect. These data suggest that the translocation of the Rho family proteins to the membrane fraction is controlled by their activation state and that agonists show selectivity in inducing the activation/translocation of these proteins.

KW - 3T3 Cells

KW - Animals

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KW - Endothelin-1

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KW - GTPase-Activating Proteins

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