Dirigent domain-containing protein is part of the machinery required for formation of the lignin-based Casparian strip in the root

Prashant S Hosmani, Takehiro Kamiya, John Danku, Sadaf Naseer, Niko Geldner, Mary Lou Guerinot, David E Salt

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The endodermis acts as a "second skin" in plant roots by providing the cellular control necessary for the selective entry of water and solutes into the vascular system. To enable such control, Casparian strips span the cell wall of adjacent endodermal cells to form a tight junction that blocks extracellular diffusion across the endodermis. This junction is composed of lignin that is polymerized by oxidative coupling of monolignols through the action of a NADPH oxidase and peroxidases. Casparian strip domain proteins (CASPs) correctly position this biosynthetic machinery by forming a protein scaffold in the plasma membrane at the site where the Casparian strip forms. Here, we show that the dirigent-domain containing protein, enhanced suberin1 (ESB1), is part of this machinery, playing an essential role in the correct formation of Casparian strips. ESB1 is localized to Casparian strips in a CASP-dependent manner, and in the absence of ESB1, disordered and defective Casparian strips are formed. In addition, loss of ESB1 disrupts the localization of the CASP1 protein at the casparian strip domain, suggesting a reciprocal requirement for both ESB1 and CASPs in forming the casparian strip domain.
Original languageEnglish
Pages (from-to)14498-14503
Number of pages6
JournalPNAS
Volume110
Issue number35
Early online date12 Aug 2013
DOIs
Publication statusPublished - 27 Aug 2013

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Lignin
Oxidative Coupling
Plant Roots
Tight Junctions
NADPH Oxidase
Cell Wall
Blood Vessels
Proteins
Cell Membrane
Skin
Protein Domains
Water

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Dirigent domain-containing protein is part of the machinery required for formation of the lignin-based Casparian strip in the root. / Hosmani, Prashant S; Kamiya, Takehiro; Danku, John; Naseer, Sadaf; Geldner, Niko; Guerinot, Mary Lou; Salt, David E.

In: PNAS, Vol. 110, No. 35, 27.08.2013, p. 14498-14503.

Research output: Contribution to journalArticle

Hosmani, Prashant S ; Kamiya, Takehiro ; Danku, John ; Naseer, Sadaf ; Geldner, Niko ; Guerinot, Mary Lou ; Salt, David E. / Dirigent domain-containing protein is part of the machinery required for formation of the lignin-based Casparian strip in the root. In: PNAS. 2013 ; Vol. 110, No. 35. pp. 14498-14503.
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