Downsizing Class II Lasso Peptides: Genome Mining-Guided Isolation of Huascopeptin Containing the First Gly1-Asp7 Macrocycle

Carlos Cortés-Albayay*, Scott A Jarmusch, Franziska Trusch, Rainer Ebel, Barbara A Andrews, Marcel Jaspars, Juan A Asenjo*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

A new lasso peptide, huascopeptin, was isolated following genome-mined discovery of a new biosynthetic gene cluster in extremotolerant Streptomyces huasconensis HST28T from Salar de Huasco, Atacama Desert, Chile. Compound 1 is a 13-residue class II lasso peptide containing a novel Gly1-Asp7 macrolactam ring, a three-residue loop, and a three-residue tail, making it the smallest lasso peptide isolated to date. The lasso structure was confirmed using NOE restraint-based molecular dynamics simulations.

Original languageEnglish
Pages (from-to)1661-1667
Number of pages7
JournalJournal of Organic Chemistry
Volume85
Issue number3
Early online date13 Jan 2020
DOIs
Publication statusPublished - 7 Feb 2020

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