Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer

Seyedeh Afrooz Azimi; Hamid Reza Sadegh Nia; Alireza Mosavi Jarrahi; Hamid Reza Jamaati; Mehdi Kazempour Dizaji; Hossein Dargahi; Naghmeh Bahrami; Alireza Pasdar; Adnan Khosravi; Naeim Bahrami; Shadi Daghighi; Abdolreza Mohamadnia

doi:10.5812/ijcm.85456

Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer

Seyedeh Afrooz Azimi, Hamid Reza Sadegh Nia, Alireza Mosavi Jarrahi, Hamid Reza Jamaati, Mehdi Kazempour Dizaji, Hossein Dargahi, Naghmeh Bahrami, Alireza Pasdar, Adnan Khosravi, Naeim Bahrami, Shadi Daghighi, Abdolreza Mohamadnia^*

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

2 Downloads (Pure)

Abstract

Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released from tumors, can be detected in circulating blood. Objectives: The aim of this study was to examine the expression of miRNA-205 and-21 in lung cancer patients relative to healthy controls. Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC) blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated, using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test. Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls (6.8 ± 0.42 and 1.2 ± 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 ± 0.52) significantly increased compared to the control group (1.6 ± 0.14) (P < 0.001). Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key roles in impaired apoptosis and further cancerous progression.

Original language	English
Article number	e85456
Number of pages	6
Journal	International Journal of Cancer Management
Volume	12
Issue number	1
Early online date	19 Jan 2019
DOIs	https://doi.org/10.5812/ijcm.85456
Publication status	Published - 2019

Bibliographical note

This research is a part of the efforts of the professors and colleagues of Masih Daneshvari Hospital of Shahid Beheshti University of Medical Sciences and Mashhad Medical Sciences University. All involved are sincerely thanked.

Keywords

Expression
MiR-205
MiR-21
Non-Small Cell Lung Cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

@article{baf5c49e85e94e7da2367a6865f661fa,

title = "Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer",

abstract = "Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released from tumors, can be detected in circulating blood. Objectives: The aim of this study was to examine the expression of miRNA-205 and-21 in lung cancer patients relative to healthy controls. Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC) blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated, using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test. Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls (6.8 ± 0.42 and 1.2 ± 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 ± 0.52) significantly increased compared to the control group (1.6 ± 0.14) (P < 0.001). Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key roles in impaired apoptosis and further cancerous progression.",

keywords = "Expression, MiR-205, MiR-21, Non-Small Cell Lung Cancer",

author = "Azimi, {Seyedeh Afrooz} and Nia, {Hamid Reza Sadegh} and Jarrahi, {Alireza Mosavi} and Jamaati, {Hamid Reza} and Dizaji, {Mehdi Kazempour} and Hossein Dargahi and Naghmeh Bahrami and Alireza Pasdar and Adnan Khosravi and Naeim Bahrami and Shadi Daghighi and Abdolreza Mohamadnia",

note = " This research is a part of the efforts of the professors and colleagues of Masih Daneshvari Hospital of Shahid Beheshti University of Medical Sciences and Mashhad Medical Sciences University. All involved are sincerely thanked. ",

year = "2019",

doi = "10.5812/ijcm.85456",

language = "English",

volume = "12",

journal = "International Journal of Cancer Management",

issn = "2538-4422",

publisher = "Kowsar",

number = "1",

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TY - JOUR

T1 - Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer

AU - Azimi, Seyedeh Afrooz

AU - Nia, Hamid Reza Sadegh

AU - Jarrahi, Alireza Mosavi

AU - Jamaati, Hamid Reza

AU - Dizaji, Mehdi Kazempour

AU - Dargahi, Hossein

AU - Bahrami, Naghmeh

AU - Pasdar, Alireza

AU - Khosravi, Adnan

AU - Bahrami, Naeim

AU - Daghighi, Shadi

AU - Mohamadnia, Abdolreza

N1 - This research is a part of the efforts of the professors and colleagues of Masih Daneshvari Hospital of Shahid Beheshti University of Medical Sciences and Mashhad Medical Sciences University. All involved are sincerely thanked.

PY - 2019

Y1 - 2019

N2 - Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released from tumors, can be detected in circulating blood. Objectives: The aim of this study was to examine the expression of miRNA-205 and-21 in lung cancer patients relative to healthy controls. Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC) blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated, using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test. Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls (6.8 ± 0.42 and 1.2 ± 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 ± 0.52) significantly increased compared to the control group (1.6 ± 0.14) (P < 0.001). Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key roles in impaired apoptosis and further cancerous progression.

AB - Background: Lung cancer is the leading cause of death, related to cancer in both men and women. MicroRNAs (miRNAs) play key roles in regulating the genes that are responsible for apoptosis, and regulation of gene expression. MicroRNA expression is deregulated in cancer. Many efforts have been made to detect cancer biomarkers. Extracellular miRNAs, which are directly released from tumors, can be detected in circulating blood. Objectives: The aim of this study was to examine the expression of miRNA-205 and-21 in lung cancer patients relative to healthy controls. Methods: This cross sectional study was performed in 50 healthy blood sample controls and 50 non-small cell lung cancer (NSCLC) blood samples. The expression of two miRNAs (miR-21 and miR-205) in peripheral blood mononuclear cells (PBMCs) was evaluated, using real-time polymerase chain reaction (real-time PCR) techniques. Statistical tests were carried out via the Mann-Whitney test. Results: The miR-205 levels of expression in the patients with lung cancer significantly increased in comparison to healthy controls (6.8 ± 0.42 and 1.2 ± 0.19, respectively), (P = 0.014). The expression of miR-21 in lung cancer (5.2 ± 0.52) significantly increased compared to the control group (1.6 ± 0.14) (P < 0.001). Conclusions: Based on our findings, lung cancer can enhance the ectopic expression of several miRNAs, which, in turn, play key roles in impaired apoptosis and further cancerous progression.

KW - Expression

KW - MiR-205

KW - MiR-21

KW - Non-Small Cell Lung Cancer

UR - http://www.scopus.com/inward/record.url?scp=85068822269&partnerID=8YFLogxK

U2 - 10.5812/ijcm.85456

DO - 10.5812/ijcm.85456

M3 - Article

AN - SCOPUS:85068822269

SN - 2538-4422

VL - 12

JO - International Journal of Cancer Management

JF - International Journal of Cancer Management

IS - 1

M1 - e85456

ER -

Ectopic expression of miRNA-21 and miRNA-205 in non-small cell lung cancer

Abstract

Bibliographical note

Keywords

UN SDGs

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