Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial

Harriet Carroll (Corresponding Author), Iain Templeman, Yung-chih Chen, Robert M. Edinburgh, Elaine K. Burch, Jake T. Jewitt, Georgie Povey, Timothy D. Robinson, William L. Dooley, Robert Jones, Kostas Tsintzas, Widet Gallo, Olle Melander, Dylan Thompson, Lewis J James, Laura Johnson, James A. Betts

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 male) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated, after four days of pre-trial standardization. One day pre-OGTT, participants were dehydrated for 1-h in a heat-tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measures and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken pre- and post-intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9±1.2% body mass loss, 2.9±2.7% cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all p≤0.007). Fasted serum glucose (HYPO 5.10±0.42 mmol∙l‑1; RE 5.02±0.40 mmol∙l-1; p=0.327) and insulin (HYPO 27.1±9.7 pmol∙L-1; RE 27.6±9.2 pmol∙L-1; p=0.809) concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose ( p=0.627) or insulin ( p=0.200) responses during the OGTT. Muscle water content was lower pre-OGTT after HYPO compared to RE (761±13 g∙kg-1 wet weight versus 772±18 g∙kg-1 RE), but similar post-OGTT (HYPO 779±15 g∙kg-1 versus RE 780±20 g∙kg‑1; time p=0.011; trial*time p = 0.055). Resting energy expenditure was similar between hydration states (stable between -1.21 and 5.94 kJ∙kg-1∙d-1; trial p=0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.
Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalJournal of Applied Physiology
Volume126
Issue number2
Early online date1 Feb 2019
DOIs
Publication statusPublished - 20 Feb 2019

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Glucose Tolerance Test
Cross-Over Studies
Cell Size
Muscles
Computer Peripherals
Insulin
Glucose
Water
Thigh
Serum
Osmolar Concentration
Energy Metabolism
Hot Temperature
Biomarkers
Tomography
Biopsy
Weights and Measures

Keywords

  • copeptin
  • glycemia
  • health
  • hydration
  • metabolism
  • OXIDATION
  • MUSCLE
  • RISK
  • ENERGY-INTAKE
  • VASOPRESSIN RECEPTOR
  • PLAIN WATER-INTAKE
  • HYDRATION STATUS
  • FLUID
  • GLUCOSE
  • PLASMA COPEPTIN

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Effect of acute hypohydration on glycemic regulation in healthy adults : a randomized crossover trial . / Carroll, Harriet (Corresponding Author); Templeman, Iain; Chen, Yung-chih; Edinburgh, Robert M.; Burch, Elaine K.; Jewitt, Jake T.; Povey, Georgie; Robinson, Timothy D.; Dooley, William L.; Jones, Robert; Tsintzas, Kostas; Gallo, Widet; Melander, Olle; Thompson, Dylan; James, Lewis J; Johnson, Laura; Betts, James A.

In: Journal of Applied Physiology, Vol. 126, No. 2, 20.02.2019, p. 422-430.

Research output: Contribution to journalArticle

Carroll, H, Templeman, I, Chen, Y, Edinburgh, RM, Burch, EK, Jewitt, JT, Povey, G, Robinson, TD, Dooley, WL, Jones, R, Tsintzas, K, Gallo, W, Melander, O, Thompson, D, James, LJ, Johnson, L & Betts, JA 2019, 'Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial ' Journal of Applied Physiology, vol. 126, no. 2, pp. 422-430. https://doi.org/10.1152/japplphysiol.00771.2018
Carroll, Harriet ; Templeman, Iain ; Chen, Yung-chih ; Edinburgh, Robert M. ; Burch, Elaine K. ; Jewitt, Jake T. ; Povey, Georgie ; Robinson, Timothy D. ; Dooley, William L. ; Jones, Robert ; Tsintzas, Kostas ; Gallo, Widet ; Melander, Olle ; Thompson, Dylan ; James, Lewis J ; Johnson, Laura ; Betts, James A. / Effect of acute hypohydration on glycemic regulation in healthy adults : a randomized crossover trial . In: Journal of Applied Physiology. 2019 ; Vol. 126, No. 2. pp. 422-430.
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AU - Jewitt, Jake T.

AU - Povey, Georgie

AU - Robinson, Timothy D.

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AU - Gallo, Widet

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AU - Thompson, Dylan

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AU - Johnson, Laura

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KW - VASOPRESSIN RECEPTOR

KW - PLAIN WATER-INTAKE

KW - HYDRATION STATUS

KW - FLUID

KW - GLUCOSE

KW - PLASMA COPEPTIN

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