Effect of ammonia concentration on the composition, hydrolytic activity and nitrogen metabolism of the microbial flora of the rumen

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The mean NH3 concentration in the rumen of sheep fed whole barley (0d̀8 kg/d) by continuous feeders was increased from 6d̀1 to 13d̀4 HIM by supplementing the feed with urea (30 g/kg). This change caused a 90% increase in the rate of degradation of rolled barley, and smaller increases in the rates of degradation of protein and plant fibre in the rumen. The total viable count and numbers of pectinolytic bacteria in rumen fluid increased with the urea supplement. Enzyme studies indicated that NAD-linked glutamate dehydrogenase was the main pathway of NH3 assimilation by rumen bacteria at both NH3 concentrations. Glutamate was the main free amino acid found in the rumen at low NH3 but, despite the low activity of alanine dehydrogenase and glutamate-pyruvate aminotransferase, alanine was the principal amino acid at high NH3 concentrations. Hydrolytic rumen bacteria may require the higher NH3 concentration either for effective NH3 assimilation by an unknown mechanism involving alanine or for full expression of enzyme activity.
Original languageEnglish
Pages (from-to)443-455
Number of pages13
JournalJournal of Applied Bacteriology
Volume47
Issue number3
DOIs
Publication statusPublished - Dec 1979

Fingerprint

Rumen
Ammonia
Nitrogen
Hordeum
Bacteria
Alanine
Urea
Alanine Dehydrogenase
Amino Acids
Glutamate Dehydrogenase
Plant Proteins
Enzymes
NAD
Proteolysis
Glutamic Acid
Sheep

Cite this

@article{ffb0d4916b8a4663ae963d80cf8f8632,
title = "Effect of ammonia concentration on the composition, hydrolytic activity and nitrogen metabolism of the microbial flora of the rumen",
abstract = "The mean NH3 concentration in the rumen of sheep fed whole barley (0d̀8 kg/d) by continuous feeders was increased from 6d̀1 to 13d̀4 HIM by supplementing the feed with urea (30 g/kg). This change caused a 90{\%} increase in the rate of degradation of rolled barley, and smaller increases in the rates of degradation of protein and plant fibre in the rumen. The total viable count and numbers of pectinolytic bacteria in rumen fluid increased with the urea supplement. Enzyme studies indicated that NAD-linked glutamate dehydrogenase was the main pathway of NH3 assimilation by rumen bacteria at both NH3 concentrations. Glutamate was the main free amino acid found in the rumen at low NH3 but, despite the low activity of alanine dehydrogenase and glutamate-pyruvate aminotransferase, alanine was the principal amino acid at high NH3 concentrations. Hydrolytic rumen bacteria may require the higher NH3 concentration either for effective NH3 assimilation by an unknown mechanism involving alanine or for full expression of enzyme activity.",
author = "Wallace, {R. John}",
note = "DA - 19800616 IS - 0021-8847 (Print) IS - 0021-8847 (Linking) LA - eng PT - Journal Article RN - 0 (Amino Acids) RN - 57-13-6 (Urea) RN - 7664-41-7 (Ammonia) RN - 7727-37-9 (Nitrogen) RN - EC 1.1.- (Carbohydrate Dehydrogenases) RN - EC 1.4.1.2 (Glutamate Dehydrogenase) SB - IM",
year = "1979",
month = "12",
doi = "10.1111/j.1365-2672.1979.tb01205.x",
language = "English",
volume = "47",
pages = "443--455",
journal = "Journal of Applied Bacteriology",
issn = "0021-8847",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Effect of ammonia concentration on the composition, hydrolytic activity and nitrogen metabolism of the microbial flora of the rumen

AU - Wallace, R. John

N1 - DA - 19800616 IS - 0021-8847 (Print) IS - 0021-8847 (Linking) LA - eng PT - Journal Article RN - 0 (Amino Acids) RN - 57-13-6 (Urea) RN - 7664-41-7 (Ammonia) RN - 7727-37-9 (Nitrogen) RN - EC 1.1.- (Carbohydrate Dehydrogenases) RN - EC 1.4.1.2 (Glutamate Dehydrogenase) SB - IM

PY - 1979/12

Y1 - 1979/12

N2 - The mean NH3 concentration in the rumen of sheep fed whole barley (0d̀8 kg/d) by continuous feeders was increased from 6d̀1 to 13d̀4 HIM by supplementing the feed with urea (30 g/kg). This change caused a 90% increase in the rate of degradation of rolled barley, and smaller increases in the rates of degradation of protein and plant fibre in the rumen. The total viable count and numbers of pectinolytic bacteria in rumen fluid increased with the urea supplement. Enzyme studies indicated that NAD-linked glutamate dehydrogenase was the main pathway of NH3 assimilation by rumen bacteria at both NH3 concentrations. Glutamate was the main free amino acid found in the rumen at low NH3 but, despite the low activity of alanine dehydrogenase and glutamate-pyruvate aminotransferase, alanine was the principal amino acid at high NH3 concentrations. Hydrolytic rumen bacteria may require the higher NH3 concentration either for effective NH3 assimilation by an unknown mechanism involving alanine or for full expression of enzyme activity.

AB - The mean NH3 concentration in the rumen of sheep fed whole barley (0d̀8 kg/d) by continuous feeders was increased from 6d̀1 to 13d̀4 HIM by supplementing the feed with urea (30 g/kg). This change caused a 90% increase in the rate of degradation of rolled barley, and smaller increases in the rates of degradation of protein and plant fibre in the rumen. The total viable count and numbers of pectinolytic bacteria in rumen fluid increased with the urea supplement. Enzyme studies indicated that NAD-linked glutamate dehydrogenase was the main pathway of NH3 assimilation by rumen bacteria at both NH3 concentrations. Glutamate was the main free amino acid found in the rumen at low NH3 but, despite the low activity of alanine dehydrogenase and glutamate-pyruvate aminotransferase, alanine was the principal amino acid at high NH3 concentrations. Hydrolytic rumen bacteria may require the higher NH3 concentration either for effective NH3 assimilation by an unknown mechanism involving alanine or for full expression of enzyme activity.

U2 - 10.1111/j.1365-2672.1979.tb01205.x

DO - 10.1111/j.1365-2672.1979.tb01205.x

M3 - Article

VL - 47

SP - 443

EP - 455

JO - Journal of Applied Bacteriology

JF - Journal of Applied Bacteriology

SN - 0021-8847

IS - 3

ER -