Effect of inulin on the human gut microbiota: stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii

Carlett Ramirez-Farias, Kathleen Slezak, Zoe Fuller, Alan Stuart Duncan, Grietje Holtrop, Petra Louis (Corresponding Author)

Research output: Contribution to journalArticle

408 Citations (Scopus)

Abstract

Prebiotics are food ingredients that improve health by modulating the colonic microbiota. The bifidogenic effect of the prebiotic inulin is well established, however, it remains unclear which species of Bifidobacterium are stimulated in vivo and whether bacteria] groups other than lactic acid bacteria are affected by inulin consumption. Changes in the faecal microbiota composition were examined by real-time PCR in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in comparison with a control period without ally supplement intake. The prevalence of most bacterial groups examined did not change after inulin intake, although the low G + C % Gram-positive species Faecali-bacterium prausnitzii exhibited a significant increase (10.3 % for control period v. 14.5 % during inulin intake, P=0.019). The composition of the genus Bifidobacterium was studied in four of the volunteers by clone library analysis. Between three and five Bifidobacterium spp. were found in each volunteer. Bifidobacterium adolescentis and Bifidobacterium longum were present in all volunteers, and Bifidobacterium pseudocatenulatum, Bifidobacterium animalis, Bifidobacterium bifidium and Bifidobacterium dentitum were also detected. Real-time PCR was employed to quantify the four most prevalent Bifidobacterium spp., B. adolescentis, B. longum, B. pseudocatenulatum and B. bifidum, in ten volunteers carrying detectable levels of bifidobacteria. B. adolescentis showed the strongest response to inulin consumption, increasing from 0.89 to 3.9 % of the total microbiota (P=0.001). B. bifidum was increased from 0.22 to 0.63% (P<0.001) for the five volunteers for whom this species was present.

Original languageEnglish
Pages (from-to)541-550
Number of pages10
JournalBritish Journal of Nutrition
Volume101
Issue number4
DOIs
Publication statusPublished - 28 Feb 2009

Keywords

  • Prebiotic
  • Inulin
  • Microbiota
  • Bifidobacterium
  • Butyrate-producing bacteria
  • Real-time PCR
  • Multiple sequence alignment
  • Species-specific primers
  • Human fecal flora
  • Human feces
  • Fusobacterium-prausnitzii
  • Fermentation products
  • Bacteroides-fragilis
  • Targeted primers
  • prebiotic
  • inulin
  • microbiota

Cite this

Effect of inulin on the human gut microbiota : stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii. / Ramirez-Farias, Carlett; Slezak, Kathleen; Fuller, Zoe; Duncan, Alan Stuart; Holtrop, Grietje; Louis, Petra (Corresponding Author).

In: British Journal of Nutrition, Vol. 101, No. 4, 28.02.2009, p. 541-550.

Research output: Contribution to journalArticle

Ramirez-Farias, Carlett ; Slezak, Kathleen ; Fuller, Zoe ; Duncan, Alan Stuart ; Holtrop, Grietje ; Louis, Petra. / Effect of inulin on the human gut microbiota : stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii. In: British Journal of Nutrition. 2009 ; Vol. 101, No. 4. pp. 541-550.
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AB - Prebiotics are food ingredients that improve health by modulating the colonic microbiota. The bifidogenic effect of the prebiotic inulin is well established, however, it remains unclear which species of Bifidobacterium are stimulated in vivo and whether bacteria] groups other than lactic acid bacteria are affected by inulin consumption. Changes in the faecal microbiota composition were examined by real-time PCR in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in comparison with a control period without ally supplement intake. The prevalence of most bacterial groups examined did not change after inulin intake, although the low G + C % Gram-positive species Faecali-bacterium prausnitzii exhibited a significant increase (10.3 % for control period v. 14.5 % during inulin intake, P=0.019). The composition of the genus Bifidobacterium was studied in four of the volunteers by clone library analysis. Between three and five Bifidobacterium spp. were found in each volunteer. Bifidobacterium adolescentis and Bifidobacterium longum were present in all volunteers, and Bifidobacterium pseudocatenulatum, Bifidobacterium animalis, Bifidobacterium bifidium and Bifidobacterium dentitum were also detected. Real-time PCR was employed to quantify the four most prevalent Bifidobacterium spp., B. adolescentis, B. longum, B. pseudocatenulatum and B. bifidum, in ten volunteers carrying detectable levels of bifidobacteria. B. adolescentis showed the strongest response to inulin consumption, increasing from 0.89 to 3.9 % of the total microbiota (P=0.001). B. bifidum was increased from 0.22 to 0.63% (P<0.001) for the five volunteers for whom this species was present.

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