Effects of a Lactobacillus paracasei B21060 based synbiotic on steatosis, insulin signaling and toll-like receptor expression in rats fed a high-fat diet

Giuseppina Mattace Raso, Raffaele Simeoli, Anna Iacono, Anna Santoro, Paola Amero, Orlando Paciello, Roberto Russo, Giuseppe D'Agostino, Margherita Di Costanzo, Roberto Berni Canani, Antonio Calignano, Rosaria Meli

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Insulin resistance (IR) has been identified as crucial pathophysiological factor in the development and progression of non-alcoholic fatty liver disease (NAFLD). Although mounting evidence suggests that perturbation of gut microflora exacerbates the severity of chronic liver diseases, therapeutic approaches using synbiotic has remained overlooked. Here, we show that a synbiotic composed by Lactobacillus paracasei B21060 plus arabinogalactan and fructo-oligosaccharides lessens NAFLD progression in a rat model of high fat feeding. IR and steatosis were induced by administration of high fat diet (HFD) for 6 weeks. Steatosis and hepatic inflammation, Toll-like receptor (TLR) pattern, glucose tolerance, insulin signaling and gut permeability were studied. Liver inflammatory markers were down-regulated in rats receiving the synbiotic, along with an increased expression of nuclear peroxisome proliferator-activated receptors and expression of downstream target genes. The synbiotic improved many aspects of IR, such as fasting response, hormonal homeostasis and glycemic control. Indeed it prevented the impairment of hepatic insulin signaling, reducing the phosphorylation of insulin receptor substrate-1 in Ser 307 and down-regulating suppressor of cytokine signaling 3. Gene expression analysis revealed that in the liver the synbiotic reduced cytokines synthesis and restored the HFD-dysregulated TLR 2, 4 and 9 mRNAs toward a physiological level of expression. The synbiotic preserved gut barrier integrity and reduced the relative amount of Gram-negative Enterobacteriales and Escherichia coli in colonic mucosa. Overall, our data indicate that the L. paracasei B21060 based synbiotic is effective in reducing the severity of liver injury and IR associated with high fat intake, suggesting its possible therapeutic/preventive clinical utilization.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalThe Journal of Nutritional Biochemistry
Volume25
Issue number1
Early online date10 Oct 2013
DOIs
Publication statusPublished - Jan 2014

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Synbiotics
Toll-Like Receptors
High Fat Diet
Nutrition
Liver
Rats
Fats
Insulin
Insulin Resistance
Cytokines
Insulin Receptor Substrate Proteins
Toll-Like Receptor 2
Peroxisome Proliferator-Activated Receptors
Phosphorylation
Toll-Like Receptor 9
Toll-Like Receptor 4
Mountings
Oligosaccharides
Gene expression
Escherichia coli

Keywords

  • Adiponectin
  • Adipose Tissue
  • Animals
  • Diet, High-Fat
  • Down-Regulation
  • Enterobacteriaceae
  • Fatty Liver
  • Galactans
  • Glucose Transporter Type 4
  • Insulin Receptor Substrate Proteins
  • Insulin Resistance
  • Intestinal Mucosa
  • Lactobacillus
  • Liver
  • Male
  • Oligosaccharides
  • PPAR gamma
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction
  • Suppressor of Cytokine Signaling Proteins
  • Synbiotics
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptor 9

Cite this

Effects of a Lactobacillus paracasei B21060 based synbiotic on steatosis, insulin signaling and toll-like receptor expression in rats fed a high-fat diet. / Raso, Giuseppina Mattace; Simeoli, Raffaele; Iacono, Anna; Santoro, Anna; Amero, Paola; Paciello, Orlando; Russo, Roberto; D'Agostino, Giuseppe; Di Costanzo, Margherita; Canani, Roberto Berni; Calignano, Antonio; Meli, Rosaria.

In: The Journal of Nutritional Biochemistry, Vol. 25, No. 1, 01.2014, p. 81-90.

Research output: Contribution to journalArticle

Raso, GM, Simeoli, R, Iacono, A, Santoro, A, Amero, P, Paciello, O, Russo, R, D'Agostino, G, Di Costanzo, M, Canani, RB, Calignano, A & Meli, R 2014, 'Effects of a Lactobacillus paracasei B21060 based synbiotic on steatosis, insulin signaling and toll-like receptor expression in rats fed a high-fat diet', The Journal of Nutritional Biochemistry, vol. 25, no. 1, pp. 81-90. https://doi.org/10.1016/j.jnutbio.2013.09.006
Raso, Giuseppina Mattace ; Simeoli, Raffaele ; Iacono, Anna ; Santoro, Anna ; Amero, Paola ; Paciello, Orlando ; Russo, Roberto ; D'Agostino, Giuseppe ; Di Costanzo, Margherita ; Canani, Roberto Berni ; Calignano, Antonio ; Meli, Rosaria. / Effects of a Lactobacillus paracasei B21060 based synbiotic on steatosis, insulin signaling and toll-like receptor expression in rats fed a high-fat diet. In: The Journal of Nutritional Biochemistry. 2014 ; Vol. 25, No. 1. pp. 81-90.
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AU - Amero, Paola

AU - Paciello, Orlando

AU - Russo, Roberto

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AU - Canani, Roberto Berni

AU - Calignano, Antonio

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