Effects of allergic diseases and age on the composition of serum IgG glycome in children

Marija Pezer, Jerko Stambuk, Marija Perica, Genadij Razdorov, Ivana Banic, Frano Vuckovic, Adrijana Miletic Gospic, Ivo Ugrina, Ana Vecenaj, Maja Pucic Bakovic, Sandra Bulat Lokas, Jelena Zivkovic, Davor Plavec, Graham Devereux, Mirjana Turkalj, Gordan Lauc

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Abstract

It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having a role in IgG modulation of allergic response. This is the first study investigating patients' IgG glycosylation profile in allergic diseases. Subclass specific IgG glycosylation profile was analyzed in two cohorts of allergen sensitized and non-sensitized 3- to 11-year-old children (conducted at University of Aberdeen, UK and Children's Hospital Srebrnjak, Zagreb, Croatia) with 893 subjects in total. IgG was isolated from serum/plasma by affinity chromatography on Protein G. IgG tryptic glycopeptides were analyzed by liquid chromatography electrospray ionization mass spectrometry. In the Zagreb cohort IgG glycome composition changed with age across all IgG subclasses. In both cohorts, IgG glycome composition did not differ in allergen sensitized subjects, nor children sensitized to individual allergens, single allergen mean wheal diameter or positive wheal sum values. In the Zagreb study the results were also replicated for high total serum IgE and in children with self-reported manifest allergic disease. In conclusion, our findings demonstrate no association between serum IgG glycome composition and allergic diseases in children.

Original languageEnglish
Article number33198
Pages (from-to)1-10
Number of pages10
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 12 Sep 2016

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Immunoglobulin G
Serum
Allergens
Glycosylation
Croatia
Electrospray Ionization Mass Spectrometry
Glycopeptides
Affinity Chromatography
Liquid Chromatography
Immunoglobulin E
Polysaccharides
Hypersensitivity

Keywords

  • antibodies
  • glycobiology
  • glycosylation
  • mass spectrometry
  • paediatric research

Cite this

Pezer, M., Stambuk, J., Perica, M., Razdorov, G., Banic, I., Vuckovic, F., ... Lauc, G. (2016). Effects of allergic diseases and age on the composition of serum IgG glycome in children. Scientific Reports, 6, 1-10. [33198]. https://doi.org/10.1038/srep33198

Effects of allergic diseases and age on the composition of serum IgG glycome in children. / Pezer, Marija; Stambuk, Jerko; Perica, Marija; Razdorov, Genadij; Banic, Ivana; Vuckovic, Frano; Gospic, Adrijana Miletic; Ugrina, Ivo; Vecenaj, Ana; Bakovic, Maja Pucic; Lokas, Sandra Bulat; Zivkovic, Jelena; Plavec, Davor; Devereux, Graham; Turkalj, Mirjana; Lauc, Gordan.

In: Scientific Reports, Vol. 6, 33198, 12.09.2016, p. 1-10.

Research output: Contribution to journalArticle

Pezer, M, Stambuk, J, Perica, M, Razdorov, G, Banic, I, Vuckovic, F, Gospic, AM, Ugrina, I, Vecenaj, A, Bakovic, MP, Lokas, SB, Zivkovic, J, Plavec, D, Devereux, G, Turkalj, M & Lauc, G 2016, 'Effects of allergic diseases and age on the composition of serum IgG glycome in children', Scientific Reports, vol. 6, 33198, pp. 1-10. https://doi.org/10.1038/srep33198
Pezer M, Stambuk J, Perica M, Razdorov G, Banic I, Vuckovic F et al. Effects of allergic diseases and age on the composition of serum IgG glycome in children. Scientific Reports. 2016 Sep 12;6:1-10. 33198. https://doi.org/10.1038/srep33198
Pezer, Marija ; Stambuk, Jerko ; Perica, Marija ; Razdorov, Genadij ; Banic, Ivana ; Vuckovic, Frano ; Gospic, Adrijana Miletic ; Ugrina, Ivo ; Vecenaj, Ana ; Bakovic, Maja Pucic ; Lokas, Sandra Bulat ; Zivkovic, Jelena ; Plavec, Davor ; Devereux, Graham ; Turkalj, Mirjana ; Lauc, Gordan. / Effects of allergic diseases and age on the composition of serum IgG glycome in children. In: Scientific Reports. 2016 ; Vol. 6. pp. 1-10.
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abstract = "It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having a role in IgG modulation of allergic response. This is the first study investigating patients' IgG glycosylation profile in allergic diseases. Subclass specific IgG glycosylation profile was analyzed in two cohorts of allergen sensitized and non-sensitized 3- to 11-year-old children (conducted at University of Aberdeen, UK and Children's Hospital Srebrnjak, Zagreb, Croatia) with 893 subjects in total. IgG was isolated from serum/plasma by affinity chromatography on Protein G. IgG tryptic glycopeptides were analyzed by liquid chromatography electrospray ionization mass spectrometry. In the Zagreb cohort IgG glycome composition changed with age across all IgG subclasses. In both cohorts, IgG glycome composition did not differ in allergen sensitized subjects, nor children sensitized to individual allergens, single allergen mean wheal diameter or positive wheal sum values. In the Zagreb study the results were also replicated for high total serum IgE and in children with self-reported manifest allergic disease. In conclusion, our findings demonstrate no association between serum IgG glycome composition and allergic diseases in children.",
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note = "Acknowledgements Glycan analysis was partly supported by European Commission GlycoBioM (contract #259869), IBD-BIOM (contract #305479), HighGlycan (contract #278535), MIMOmics (contract #305280), HTP-GlycoMet (contract #324400) and IntegraLife (contract #315997) grants. The SEATON cohort was partly funded by the UK Medical Research Council (contract #80219) and Asthma UK (contract #00/011 and 02/017) grants.",
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AU - Stambuk, Jerko

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AU - Banic, Ivana

AU - Vuckovic, Frano

AU - Gospic, Adrijana Miletic

AU - Ugrina, Ivo

AU - Vecenaj, Ana

AU - Bakovic, Maja Pucic

AU - Lokas, Sandra Bulat

AU - Zivkovic, Jelena

AU - Plavec, Davor

AU - Devereux, Graham

AU - Turkalj, Mirjana

AU - Lauc, Gordan

N1 - Acknowledgements Glycan analysis was partly supported by European Commission GlycoBioM (contract #259869), IBD-BIOM (contract #305479), HighGlycan (contract #278535), MIMOmics (contract #305280), HTP-GlycoMet (contract #324400) and IntegraLife (contract #315997) grants. The SEATON cohort was partly funded by the UK Medical Research Council (contract #80219) and Asthma UK (contract #00/011 and 02/017) grants.

PY - 2016/9/12

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N2 - It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having a role in IgG modulation of allergic response. This is the first study investigating patients' IgG glycosylation profile in allergic diseases. Subclass specific IgG glycosylation profile was analyzed in two cohorts of allergen sensitized and non-sensitized 3- to 11-year-old children (conducted at University of Aberdeen, UK and Children's Hospital Srebrnjak, Zagreb, Croatia) with 893 subjects in total. IgG was isolated from serum/plasma by affinity chromatography on Protein G. IgG tryptic glycopeptides were analyzed by liquid chromatography electrospray ionization mass spectrometry. In the Zagreb cohort IgG glycome composition changed with age across all IgG subclasses. In both cohorts, IgG glycome composition did not differ in allergen sensitized subjects, nor children sensitized to individual allergens, single allergen mean wheal diameter or positive wheal sum values. In the Zagreb study the results were also replicated for high total serum IgE and in children with self-reported manifest allergic disease. In conclusion, our findings demonstrate no association between serum IgG glycome composition and allergic diseases in children.

AB - It is speculated that immunoglobulin G (IgG) plays a regulatory role in allergic reactions. The glycans on the Fc region are known to affect IgG effector functions, thereby possibly having a role in IgG modulation of allergic response. This is the first study investigating patients' IgG glycosylation profile in allergic diseases. Subclass specific IgG glycosylation profile was analyzed in two cohorts of allergen sensitized and non-sensitized 3- to 11-year-old children (conducted at University of Aberdeen, UK and Children's Hospital Srebrnjak, Zagreb, Croatia) with 893 subjects in total. IgG was isolated from serum/plasma by affinity chromatography on Protein G. IgG tryptic glycopeptides were analyzed by liquid chromatography electrospray ionization mass spectrometry. In the Zagreb cohort IgG glycome composition changed with age across all IgG subclasses. In both cohorts, IgG glycome composition did not differ in allergen sensitized subjects, nor children sensitized to individual allergens, single allergen mean wheal diameter or positive wheal sum values. In the Zagreb study the results were also replicated for high total serum IgE and in children with self-reported manifest allergic disease. In conclusion, our findings demonstrate no association between serum IgG glycome composition and allergic diseases in children.

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