Effects of propionate on the acid microclimate of hen (Gallus domesticus) colonic mucosa

K Holtug, G T McEwan, E Skadhauge

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

1. Short-chain fatty acid absorption in hen colon is protonated across the apical border coupled to an apical electrogenic proton pump. 2. The surface pH of the isolated colonic epithelium was 6.27 +/- 0.05, when incubated in Krebs-phosphate buffer pH 7.0. 3. Propionate 7 and 40 mmol/l in the incubation medium (pH 7.0) increased microclimate pH to 6.47 +/- 0.04 and 6.56 +/- 0.04. Inhibition of metabolic activity by potassium cyanide 1 mmol/l increased surface pH to 6.66 +/- 0.06. 4. The calculated concentration of propionic acid in the microclimate is near-linearly related to the propionate concentration. Thus, the acid microclimate is not responsible for the Michaelis-Menten like kinetics of propionate transport.
Original languageEnglish
Pages (from-to)649-52
Number of pages4
JournalComparative Biochemistry and Physiology. A, Physiology
Volume103
Issue number4
DOIs
Publication statusPublished - Dec 1992

Fingerprint

Microclimate
Propionates
Chickens
Mucous Membrane
Acids
Potassium Cyanide
Proton Pumps
Volatile Fatty Acids
Buffers
Colon
Epithelium
Phosphates

Keywords

  • Animals
  • Biological Transport, Active
  • Chickens
  • Colon
  • Female
  • Hydrogen-Ion Concentration
  • Intestinal Absorption
  • Intestinal Mucosa
  • Kinetics
  • Propionates

Cite this

Effects of propionate on the acid microclimate of hen (Gallus domesticus) colonic mucosa. / Holtug, K; McEwan, G T; Skadhauge, E.

In: Comparative Biochemistry and Physiology. A, Physiology, Vol. 103, No. 4, 12.1992, p. 649-52.

Research output: Contribution to journalArticle

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N2 - 1. Short-chain fatty acid absorption in hen colon is protonated across the apical border coupled to an apical electrogenic proton pump. 2. The surface pH of the isolated colonic epithelium was 6.27 +/- 0.05, when incubated in Krebs-phosphate buffer pH 7.0. 3. Propionate 7 and 40 mmol/l in the incubation medium (pH 7.0) increased microclimate pH to 6.47 +/- 0.04 and 6.56 +/- 0.04. Inhibition of metabolic activity by potassium cyanide 1 mmol/l increased surface pH to 6.66 +/- 0.06. 4. The calculated concentration of propionic acid in the microclimate is near-linearly related to the propionate concentration. Thus, the acid microclimate is not responsible for the Michaelis-Menten like kinetics of propionate transport.

AB - 1. Short-chain fatty acid absorption in hen colon is protonated across the apical border coupled to an apical electrogenic proton pump. 2. The surface pH of the isolated colonic epithelium was 6.27 +/- 0.05, when incubated in Krebs-phosphate buffer pH 7.0. 3. Propionate 7 and 40 mmol/l in the incubation medium (pH 7.0) increased microclimate pH to 6.47 +/- 0.04 and 6.56 +/- 0.04. Inhibition of metabolic activity by potassium cyanide 1 mmol/l increased surface pH to 6.66 +/- 0.06. 4. The calculated concentration of propionic acid in the microclimate is near-linearly related to the propionate concentration. Thus, the acid microclimate is not responsible for the Michaelis-Menten like kinetics of propionate transport.

KW - Animals

KW - Biological Transport, Active

KW - Chickens

KW - Colon

KW - Female

KW - Hydrogen-Ion Concentration

KW - Intestinal Absorption

KW - Intestinal Mucosa

KW - Kinetics

KW - Propionates

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