Efficient cell transfection with melamine-based gemini surfactants

Serena Perrone, Michele Usai, Paolo Lazzari, Steven J. Tucker, Heather M. Wallace, Matteo Zanda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Gemini surfactants consisting of two melamine scaffolds connected by a n-hexyl linker and functionalized with a 1-propylammonium polar head and a lipophilic chain having variable carbon length (from C8 to C16) were synthesized. These were then used successfully for the transfection of A549, U87 MG, and Bristol 8 cell lines with maxGFP expressing plasmid. The transfection protocol was optimized appropriately (confluence, reagent/pcDNA ratio, compaction time, and transfection time) for each cell line. Under optimized conditions, the C12 and C14 melamine gemini surfactants showed little toxicity and remarkable transfection efficiency, superior to the gold-standard Lipofectamine 2000. These reagents were also able to efficiently transfect primary DRG neurons, which are notoriously difficult to transfect. The presence of serum completely inhibited the transfection capacity of these reagents. Owing to their ready availability, straightforward synthesis, high chemical stability (even in solution), ease of use (no formulation is required), improved transfection ability, and low toxicity, melamine-based gemini surfactants are very promising reagents for cellular DNA transfection.
Original languageEnglish
Pages (from-to)176-187
Number of pages12
JournalBioconjugate Chemistry
Volume24
Issue number2
Early online date8 Jan 2013
DOIs
Publication statusPublished - 20 Feb 2013

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Melamine
Surface-Active Agents
Transfection
Surface active agents
Toxicity
Cells
Chemical stability
Scaffolds
Neurons
Compaction
DNA
Gold
Availability
Carbon
Plasmids
Cell Line
Diagnosis-Related Groups
melamine
Serum

Cite this

Efficient cell transfection with melamine-based gemini surfactants. / Perrone, Serena; Usai, Michele ; Lazzari, Paolo; Tucker, Steven J.; Wallace, Heather M.; Zanda, Matteo.

In: Bioconjugate Chemistry, Vol. 24, No. 2, 20.02.2013, p. 176-187.

Research output: Contribution to journalArticle

Perrone, S, Usai, M, Lazzari, P, Tucker, SJ, Wallace, HM & Zanda, M 2013, 'Efficient cell transfection with melamine-based gemini surfactants', Bioconjugate Chemistry, vol. 24, no. 2, pp. 176-187. https://doi.org/10.1021/bc3004292
Perrone, Serena ; Usai, Michele ; Lazzari, Paolo ; Tucker, Steven J. ; Wallace, Heather M. ; Zanda, Matteo. / Efficient cell transfection with melamine-based gemini surfactants. In: Bioconjugate Chemistry. 2013 ; Vol. 24, No. 2. pp. 176-187.
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