Efflux of Creatine Kinase from Isolated Soleus Muscle Depends on Age, Sex and Type of Exercise in Mice

Juozas Baltusnikas, Tomas Venckunas, Audrius Kilikevicius, Andrej Fokin, Aivaras Ratkevicius

Research output: Contribution to journalArticle

6 Citations (Scopus)
3 Downloads (Pure)

Abstract

Elevated plasma creatine kinase (CK) activity is often used as an indicator of exercise-induced muscle damage. Our aim was to study effects of contraction type, sex and age on CK efflux from isolated skeletal muscles of mice. The soleus muscle (SOL) of adult (7.5-month old) female C57BL/6J mice was subjected to either 100 passive stretches, isometric contractions or eccentric contractions, and muscle CK efflux was assessed after two-hour incubation in vitro. SOL of young (3-month old) male and female mice was studied after 100 eccentric contractions. For adult females, muscle CK efflux was larger (p < 0.05) after eccentric contractions than after incubation without exercise (698 ± 344 vs. 268 ± 184 mU·h−1, respectively), but smaller (p < 0.05) than for young females after the same type of exercise (1069 ± 341 mU·h−1). Eccentric exercise-induced CK efflux was larger in muscles of young males compared to young females (2046 ± 317 vs 1069 ± 341 mU · h−1, respectively, p < 0.001). Our results show that eccentric contractions induce a significant increase in muscle CK efflux immediately after exercise. Isolated muscle resistance to exercise-induced CK efflux depends on age and sex of mice.
Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalJournal of Sports Science and Medicine
Volume14
Early online date1 Jun 2015
Publication statusPublished - 2015

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Creatine Kinase
MM Form Creatine Kinase
Skeletal Muscle
Muscles
Isometric Contraction
Inbred C57BL Mouse

Keywords

  • skeletal muscle
  • eccentric contractions
  • muscle damage
  • CK activity

Cite this

Baltusnikas, J., Venckunas, T., Kilikevicius, A., Fokin, A., & Ratkevicius, A. (2015). Efflux of Creatine Kinase from Isolated Soleus Muscle Depends on Age, Sex and Type of Exercise in Mice. Journal of Sports Science and Medicine, 14, 379-385.

Efflux of Creatine Kinase from Isolated Soleus Muscle Depends on Age, Sex and Type of Exercise in Mice. / Baltusnikas, Juozas; Venckunas, Tomas; Kilikevicius, Audrius; Fokin, Andrej; Ratkevicius, Aivaras.

In: Journal of Sports Science and Medicine, Vol. 14, 2015, p. 379-385.

Research output: Contribution to journalArticle

Baltusnikas, J, Venckunas, T, Kilikevicius, A, Fokin, A & Ratkevicius, A 2015, 'Efflux of Creatine Kinase from Isolated Soleus Muscle Depends on Age, Sex and Type of Exercise in Mice', Journal of Sports Science and Medicine, vol. 14, pp. 379-385.
Baltusnikas, Juozas ; Venckunas, Tomas ; Kilikevicius, Audrius ; Fokin, Andrej ; Ratkevicius, Aivaras. / Efflux of Creatine Kinase from Isolated Soleus Muscle Depends on Age, Sex and Type of Exercise in Mice. In: Journal of Sports Science and Medicine. 2015 ; Vol. 14. pp. 379-385.
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