Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties

W Mullen, J McGinn, M E J Lean, M R MacLean, P Gardner, G Duthie, T Yokota, A Crozier

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyaniclin3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargon idin-3-sophoroside, pelargonidin-3-(2G-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M-r of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an Mr of, 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its,sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.

Original languageEnglish
Pages (from-to)5191-5196
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number18
Early online date26 Jul 2002
DOIs
Publication statusPublished - 28 Aug 2002

Keywords

  • raspberries
  • ellagitannins
  • anthocyanins
  • vitamin C
  • flavonols
  • HPLC
  • tandem mass spectrometry
  • antioxidant capacity
  • vasodilation activity
  • endothelium-dependent vasorelaxation
  • ellagic acid
  • fruit
  • storage
  • juice

Cite this

Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties. / Mullen, W ; McGinn, J ; Lean, M E J ; MacLean, M R ; Gardner, P ; Duthie, G; Yokota, T ; Crozier, A .

In: Journal of Agricultural and Food Chemistry, Vol. 50, No. 18, 28.08.2002, p. 5191-5196.

Research output: Contribution to journalArticle

Mullen, W ; McGinn, J ; Lean, M E J ; MacLean, M R ; Gardner, P ; Duthie, G ; Yokota, T ; Crozier, A . / Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties. In: Journal of Agricultural and Food Chemistry. 2002 ; Vol. 50, No. 18. pp. 5191-5196.
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AU - MacLean, M R

AU - Gardner, P

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AU - Yokota, T

AU - Crozier, A

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N2 - Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyaniclin3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargon idin-3-sophoroside, pelargonidin-3-(2G-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M-r of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an Mr of, 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its,sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.

AB - Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyaniclin3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargon idin-3-sophoroside, pelargonidin-3-(2G-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M-r of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an Mr of, 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its,sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.

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KW - vasodilation activity

KW - endothelium-dependent vasorelaxation

KW - ellagic acid

KW - fruit

KW - storage

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