Abstract
Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyaniclin3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargon idin-3-sophoroside, pelargonidin-3-(2G-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M-r of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an Mr of, 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its,sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.
Original language | English |
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Pages (from-to) | 5191-5196 |
Number of pages | 6 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 50 |
Issue number | 18 |
Early online date | 26 Jul 2002 |
DOIs | |
Publication status | Published - 28 Aug 2002 |
Keywords
- raspberries
- ellagitannins
- anthocyanins
- vitamin C
- flavonols
- HPLC
- tandem mass spectrometry
- antioxidant capacity
- vasodilation activity
- endothelium-dependent vasorelaxation
- ellagic acid
- fruit
- storage
- juice