Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties

W Mullen, J McGinn, M E J Lean, M R MacLean, P Gardner, G Duthie, T Yokota, A Crozier

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Abstract

Analysis of extracts of Glen Ample raspberries (Rubus idaeus L.) by gradient, reverse phase HPLC with diode array and tandem mass spectrometry identified eleven anthocyanins, including cyaniclin3-sophoroside, cyanidin-3-(2(G)-glucosylrutinoside), cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargon idin-3-sophoroside, pelargonidin-3-(2G-glucosylrutinoside), and pelargonidin-3-glucoside. Significant quantities of an ellagitannin, sanguiin H-6, with an M-r of 1870 were detected along with lower levels of a second ellagitannin, lambertianin C, which has an Mr of, 2804. Other phenolic compounds that were detected included trace levels of ellagic acid and its,sugar conjugates along with one kaempferol- and four quercetin-based flavonol conjugates. Fractionation by preparative HPLC revealed that sanguiin H-6 was a major contributor to the antioxidant capacity of raspberries together with vitamin C and the anthocyanins. Vasodilation activity was restricted to fractions containing lambertianin C and sanguiin H-6.

Original languageEnglish
Pages (from-to)5191-5196
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number18
Early online date26 Jul 2002
DOIs
Publication statusPublished - 28 Aug 2002

Keywords

  • raspberries
  • ellagitannins
  • anthocyanins
  • vitamin C
  • flavonols
  • HPLC
  • tandem mass spectrometry
  • antioxidant capacity
  • vasodilation activity
  • endothelium-dependent vasorelaxation
  • ellagic acid
  • fruit
  • storage
  • juice

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