Endobacteria in some ectomycorrhiza of Scots pine (Pinus sylvestris)

H. Izumi, Ian C Anderson, Ian James Alexander, Kenneth Stuart Killham, E. R. B. Moore

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (Suillus flavidus, Suillus variegatus, Russula paludosa and Russula sp.) of Scots pine (Pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of PCR-amplified rDNA intergenic spacer regions and by sequence analyses of 16S rRNA genes. Ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. Bacterial communities from surface-sterilized ectomycorrhizal root tips were different from those of ectomycorrhizal root tips without surface-sterilization for all the morphotypes studied. Endobacteria belonging to the genera Pseudomonas, Burkholderia and Bacillus were isolated from more than one ectomycorrhizal morphotype, whereas species of Rahnella, Janthinobacterium and Rhodococcus were only isolated from the single morphotypes of S. variegatus, R. paludosa and Russula sp., respectively. Some of the isolated endobacteria utilized fungal sugars more readily than typical plant sugars in carbon utilization assays.

Original languageEnglish
Pages (from-to)34-43
Number of pages9
JournalFEMS Microbiology Ecology
Volume56
DOIs
Publication statusPublished - 2006

Keywords

  • endobacteria
  • ectomycorrhiza
  • ectomycorrhizal fungi
  • 16S rRNA genes
  • TUBER-BORCHII VITTAD
  • FLUORESCENT PSEUDOMONADS
  • INTRACELLULAR BACTERIA
  • COMMUNITY STRUCTURE
  • BACILLUS-SUBTILIS
  • FOREST HUMUS
  • SP-NOV.
  • GROWTH
  • DNA
  • IDENTIFICATION

Cite this

Izumi, H., Anderson, I. C., Alexander, I. J., Killham, K. S., & Moore, E. R. B. (2006). Endobacteria in some ectomycorrhiza of Scots pine (Pinus sylvestris). FEMS Microbiology Ecology, 56, 34-43. https://doi.org/10.1111/j.1574-6941.2005.00048.x