Enzymatic formation and resolution of Holliday junctions in vitro

Berndt Marino Muller, C Jones, B Kemper, S C West

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

E. coli RecA protein promotes homologous pairing and reciprocal strand exchange reactions between duplex DNA molecules in vitro. Reaction intermediates contain Holliday junctions that are driven along the DNA at a maximal rate approaching 1000 bases per minute. T4 endonuclease VII cleaves Holliday junctions in vitro, and its inclusion in RecA-mediated reactions leads to the rapid formation of heteroduplex products. Product analysis indicates patch and splice recombinant molecules similar to those expected from in vivo recombination events. The combined formation and resolution of Holliday junctions has led us to propose a model for resolution based on the structure of RecA-DNA helices. One feature of this model is that resolution, which gives rise to the two types of recombinant product, may occur without need for isomerization of the junction.
Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalCell
Volume60
Issue number2
DOIs
Publication statusPublished - 26 Jan 1990

Fingerprint

Cruciform DNA
DNA
Rec A Recombinases
Reaction intermediates
Molecules
Escherichia coli Proteins
Isomerization
Escherichia coli
Genetic Recombination
In Vitro Techniques

Keywords

  • Bacteriophage phi X 174
  • Base Sequence
  • DNA, Circular
  • DNA, Viral
  • Endodeoxyribonucleases
  • Escherichia coli
  • Models, Structural
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes
  • Protein Conformation
  • Rec A Recombinases
  • Restriction Mapping
  • T-Phages

Cite this

Enzymatic formation and resolution of Holliday junctions in vitro. / Muller, Berndt Marino; Jones, C; Kemper, B; West, S C.

In: Cell, Vol. 60, No. 2, 26.01.1990, p. 329-336.

Research output: Contribution to journalArticle

Muller, Berndt Marino ; Jones, C ; Kemper, B ; West, S C. / Enzymatic formation and resolution of Holliday junctions in vitro. In: Cell. 1990 ; Vol. 60, No. 2. pp. 329-336.
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