Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A

Yating Liu, Li Su, Qing Fang, Jioji Tabudravu, Xiaohui Yang, Kirstie Rickaby, Laurent Trembleau, Kwaku Kyeremeh, Zixin Deng, Hai Deng* (Corresponding Author), Yi Yu (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.
Original languageEnglish
Pages (from-to)16323-16328
Number of pages6
JournalJournal of Organic Chemistry
Volume84
Issue number24
Early online date15 Nov 2019
DOIs
Publication statusPublished - 20 Dec 2019

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Metabolites
Polyketides
Biosynthesis
Bioinformatics
Scaffolds
neocarazostatin A
carbazole
Proteins

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Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A. / Liu, Yating; Su, Li; Fang, Qing; Tabudravu, Jioji; Yang, Xiaohui; Rickaby, Kirstie; Trembleau, Laurent; Kyeremeh, Kwaku; Deng, Zixin; Deng, Hai (Corresponding Author); Yu, Yi (Corresponding Author).

In: Journal of Organic Chemistry, Vol. 84, No. 24, 20.12.2019, p. 16323-16328.

Research output: Contribution to journalArticle

Liu, Y, Su, L, Fang, Q, Tabudravu, J, Yang, X, Rickaby, K, Trembleau, L, Kyeremeh, K, Deng, Z, Deng, H & Yu, Y 2019, 'Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A', Journal of Organic Chemistry, vol. 84, no. 24, pp. 16323-16328. https://doi.org/10.1021/acs.joc.9b02688
Liu, Yating ; Su, Li ; Fang, Qing ; Tabudravu, Jioji ; Yang, Xiaohui ; Rickaby, Kirstie ; Trembleau, Laurent ; Kyeremeh, Kwaku ; Deng, Zixin ; Deng, Hai ; Yu, Yi. / Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A. In: Journal of Organic Chemistry. 2019 ; Vol. 84, No. 24. pp. 16323-16328.
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abstract = "Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.",
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