Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A

Yating Liu, Li Su, Qing Fang, Jioji Tabudravu, Xiaohui Yang, Kirstie Rickaby, Laurent Trembleau, Kwaku Kyeremeh, Zixin Deng, Hai Deng* (Corresponding Author), Yi Yu (Corresponding Author)

*Corresponding author for this work

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Abstract

Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.
Original languageEnglish
Pages (from-to)16323-16328
Number of pages6
JournalJournal of Organic Chemistry
Volume84
Issue number24
Early online date15 Nov 2019
DOIs
Publication statusPublished - 20 Dec 2019

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    Liu, Y., Su, L., Fang, Q., Tabudravu, J., Yang, X., Rickaby, K., Trembleau, L., Kyeremeh, K., Deng, Z., Deng, H., & Yu, Y. (2019). Enzymatic Reconstitution and Biosynthetic Investigation of the Bacterial Carbazole Neocarazostatin A. Journal of Organic Chemistry, 84(24), 16323-16328. https://doi.org/10.1021/acs.joc.9b02688