Abstract
Tricyclic carbazole is an important scaffold in many naturally occurring metabolites, as well as valuable building blocks. Here we report the reconstitution of the ring A formation of the bacterial neocarazostatin A carbazole metabolite. We provide evidence of the involvement of two unusual aromatic polyketide proteins. This finding suggests how new enzymatic activities can be recruited to specific pathways to expand biosynthetic capacities. Finally, we leveraged our bioinformatics survey to identify the untapped capacity of carbazole biosynthesis.
Original language | English |
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Pages (from-to) | 16323-16328 |
Number of pages | 6 |
Journal | Journal of Organic Chemistry |
Volume | 84 |
Issue number | 24 |
Early online date | 15 Nov 2019 |
DOIs | |
Publication status | Published - 20 Dec 2019 |
Bibliographical note
We thank the National Key Research and Development Program of China (2018YFA0900400), the National Natural Science Foundation of China (31570033, 31811530299, and 31870035 to Y.Y.), the Royal Society-NSFC Newton Mobility Grant Award (IEC\NSFC\170617 to H.D. and Y.Y.), Biotechnology and Biological Sciences Research Council UK (H.D., K.R., and L.T., BB/R00479X/1), the ElphinstoneScholarship of University of Aberdeen (Q.F.), Leverhulme Trust-Royal Society Africa award (AA090088), and Medical Research Council UK Africa Future leader (K.K. and H.D., MR/S00520X/1) for financial support.