Establishment of normal gut microbiota is compromised under excessive hygiene conditions

Bettina Schmidt, Imke E Mulder, Corran C Musk, Rustam I Aminov, Marie Lewis, Christopher R Stokes, Mick Bailey, James I Prosser, Bhupinder P Gill, John R Pluske, Denise Kelly

Research output: Contribution to journalArticle

68 Citations (Scopus)
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Abstract

Background: Early gut colonization events are purported to have a major impact on the incidence of inflammatory and autoimmune diseases in later life. Hence, factors which influence this process may have implications for both human and animal health. Previously, we demonstrated strong influences of early-life environment gut microbiota composition in adult pigs. Here, we sought to further investigate the impact of limiting microbial during early life on the development of the pig gut microbiota.

Methodology/Principal Findings: Outdoor-and indoor-reared animals, exposed to the microbiota in their natural environment for the first two days of life, were transferred to an isolator facility and adult gut microbial diversity analyzed by 16S rRNA gene sequencing. From a total of 2,196 high-quality 16S rRNA gene sequences, 440 phylotypes identified in the outdoor group and 431 phylotypes in the indoor group. The majority of clones were assigned to the phyla Firmicutes (67.5% of all sequences), Proteobacteria (17.7%), Bacteroidetes (13.5%) and to a lesser Actinobacteria (0.1%). Although the initial maternal and environmental microbial inoculum of isolator-reared animals identical to that of their naturally-reared littermates, the microbial succession and stabilization events reported previously naturally-reared outdoor animals did not occur. In contrast, the gut microbiota of isolator-reared animals remained diverse containing a large number of distinct phylotypes.

Conclusions/Significance: The results documented here indicate that establishment and development of the normal microbiota requires continuous microbial exposure during the early stages of life and this process is compromised conditions of excessive hygiene.

Original languageEnglish
Article numbere28284
Number of pages10
JournalPloS ONE
Volume6
Issue number12
DOIs
Publication statusPublished - 2 Dec 2011

Keywords

  • vancomycin-resistant enterococci
  • streptococcus-suis type-2
  • mucosal immune-system
  • intestinal microbiota
  • postnatal-development
  • United-States
  • bacteria
  • environment
  • delivery
  • endocarditis

Cite this

Schmidt, B., Mulder, I. E., Musk, C. C., Aminov, R. I., Lewis, M., Stokes, C. R., ... Kelly, D. (2011). Establishment of normal gut microbiota is compromised under excessive hygiene conditions. PloS ONE, 6(12), [e28284]. https://doi.org/10.1371/journal.pone.0028284

Establishment of normal gut microbiota is compromised under excessive hygiene conditions. / Schmidt, Bettina; Mulder, Imke E; Musk, Corran C; Aminov, Rustam I; Lewis, Marie; Stokes, Christopher R; Bailey, Mick; Prosser, James I; Gill, Bhupinder P; Pluske, John R; Kelly, Denise.

In: PloS ONE, Vol. 6, No. 12, e28284, 02.12.2011.

Research output: Contribution to journalArticle

Schmidt, B, Mulder, IE, Musk, CC, Aminov, RI, Lewis, M, Stokes, CR, Bailey, M, Prosser, JI, Gill, BP, Pluske, JR & Kelly, D 2011, 'Establishment of normal gut microbiota is compromised under excessive hygiene conditions', PloS ONE, vol. 6, no. 12, e28284. https://doi.org/10.1371/journal.pone.0028284
Schmidt B, Mulder IE, Musk CC, Aminov RI, Lewis M, Stokes CR et al. Establishment of normal gut microbiota is compromised under excessive hygiene conditions. PloS ONE. 2011 Dec 2;6(12). e28284. https://doi.org/10.1371/journal.pone.0028284
Schmidt, Bettina ; Mulder, Imke E ; Musk, Corran C ; Aminov, Rustam I ; Lewis, Marie ; Stokes, Christopher R ; Bailey, Mick ; Prosser, James I ; Gill, Bhupinder P ; Pluske, John R ; Kelly, Denise. / Establishment of normal gut microbiota is compromised under excessive hygiene conditions. In: PloS ONE. 2011 ; Vol. 6, No. 12.
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