Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method

Paul Haggarty, J J Robinson, J Ashton, Eric Milne, Clare Lesley Adam, C E Kyle, S L Christie, A J Midwood

Research output: Contribution to journalArticle

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Abstract

Energy expenditure was estimated using the doubly-labelled water (DLW) method in summer in five free-living adult, non-pregnant, non-lactating, red deer (Cervus elaphus) hinds (weight 107.3 (sE 0.9) kg; age 6 (sE 1) years) on lowland pasture under typical farming conditions. Climatic I; conditions were monitored throughout the experiment. Errors due to H-2 losses in CH4 and faeces were calculated from previous estimates of stoichiometries. CH4 production, fractionated water loss, urinary N and O-2 consumption were estimated using an iterative approach. The water flux (rH(2)O) in these animals consuming only fresh grass was 12 (sE 0.5) kg/d, the CO2 production (rCO(2)) was 1271 (sE 40) litres/d and the mean energy expenditure was 25 (sE 0.8) MJ/d. There were no significant differences in the isotope distribution spaces and flux rates, rH(2)O, rCO(2) or energy expenditure using the multi-point or two-point approaches to calculation. The DLW-derived energy expenditure of 25 MJ/d is approximately 20% higher than the recommended intake of 21 MJ/d for adult hinds kept outdoors (Adam, 1986) and, at 757 kJ/kg(0.75) per d, one third higher than the value of 570 kJ/kg(0.75) per d for stags penned indoors (Key er al. 1984).

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalBritish Journal of Nutrition
Volume80
Issue number3
DOIs
Publication statusPublished - Sep 1998

Keywords

  • energy expenditure
  • doubly-labelled water
  • red deer
  • CO2 PRODUCTION
  • VALIDATION
  • RUMINANTS
  • DEUTERIUM
  • SAMPLES
  • Energy expenditure
  • Doubly-labelled water
  • Red deer

Cite this

Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method. / Haggarty, Paul; Robinson, J J ; Ashton, J ; Milne, Eric; Adam, Clare Lesley; Kyle, C E ; Christie, S L ; Midwood, A J .

In: British Journal of Nutrition, Vol. 80, No. 3, 09.1998, p. 263-272.

Research output: Contribution to journalArticle

Haggarty, Paul ; Robinson, J J ; Ashton, J ; Milne, Eric ; Adam, Clare Lesley ; Kyle, C E ; Christie, S L ; Midwood, A J . / Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method. In: British Journal of Nutrition. 1998 ; Vol. 80, No. 3. pp. 263-272.
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AU - Ashton, J

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AU - Christie, S L

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AB - Energy expenditure was estimated using the doubly-labelled water (DLW) method in summer in five free-living adult, non-pregnant, non-lactating, red deer (Cervus elaphus) hinds (weight 107.3 (sE 0.9) kg; age 6 (sE 1) years) on lowland pasture under typical farming conditions. Climatic I; conditions were monitored throughout the experiment. Errors due to H-2 losses in CH4 and faeces were calculated from previous estimates of stoichiometries. CH4 production, fractionated water loss, urinary N and O-2 consumption were estimated using an iterative approach. The water flux (rH(2)O) in these animals consuming only fresh grass was 12 (sE 0.5) kg/d, the CO2 production (rCO(2)) was 1271 (sE 40) litres/d and the mean energy expenditure was 25 (sE 0.8) MJ/d. There were no significant differences in the isotope distribution spaces and flux rates, rH(2)O, rCO(2) or energy expenditure using the multi-point or two-point approaches to calculation. The DLW-derived energy expenditure of 25 MJ/d is approximately 20% higher than the recommended intake of 21 MJ/d for adult hinds kept outdoors (Adam, 1986) and, at 757 kJ/kg(0.75) per d, one third higher than the value of 570 kJ/kg(0.75) per d for stags penned indoors (Key er al. 1984).

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