Estimation of Lipid Hydroperoxide Levels in Tropical Marine Macroalgae

Puja Kumari, Ravindra Pal Singh, A. J. Bijo, C. R.K. Reddy*, Bhavanath Jha

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The incipient levels of lipid hydroperoxides (LHPOs) were determined in selected green, brown, and red macroalgae by the FOX assay using hydroperoxy HPLC mix. The LHPOs contents varied between the investigated species and showed relatively low values in this study. Among the greens, it varied from 12 ± 6.2 μg · g-1 (Codium sursum) to 31.5 ± 2.8 μg · g-1 (Ulva lactuca), whereas in reds, from 5.7 ± 1.6 μg · g-1 (Gracilaria corticata) to 46.2 ± 6 μg · g-1 (Sarconema filiforme), and in browns, from 4.6 ± 4.4 μg · g-1 (Dictyota bartayresiana) to 79 ± 5.0 μg · g-1 (Sargassum tenerrimum), on fresh weight basis. These hydroperoxides represented a minor fraction of total lipids and ranged from 0.04% (S. swartzii) to 1.1% (S. tenerrimum) despite being a rich source of highly unsaturated fatty acids. The susceptibility of peroxidation was assessed by specific lipid peroxidazibility (SLP) values for macroalgal tissues. The LHPO values were found to be independent of both the PUFAs contents and their degree of unsaturation (DBI), as evident from the PCA analysis. SLP values were positively correlated with the LHPOs and negatively with DBI. The FOX assay gave ≥20-fold higher values for LHPOs as compared to the TBARS method for all the samples investigated in this study. Furthermore, U. lactuca cultured in artificial seawater (ASW) enriched with nutrients (N, P, and NP) showed a sharp decline in LHPOs contents relative to those cultured in ASW alone P ≤ 0.05. It is inferred from this study that the FOX assay is an efficient, rapid, sensitive, and inexpensive technique for detecting the incipient lipid peroxidation in macroalgal tissues.

Original languageEnglish
Pages (from-to)1362-1373
Number of pages12
JournalJournal of Phycology
Volume48
Issue number6
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Fatty acid
  • FOX
  • Lipid hydroperoxides
  • Macroalgae
  • TBARS

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