Evaluation of interactive toxicity of chlorophenols in water and soil using lux-marked biosensors

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Abstract

An assessment of the toxicity of three chlorophenol compounds (2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP)), individually and in combinations, was made in deionised water, soil extracts and soils using the lux-marked microbial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. These biosensors responded to the bioavailable fraction of pollutants enabling a rapid and ecologically relevant toxicity test. Toxicity interaction responses of pollutant mixtures were predicted after individual compounds were assessed. Synergistic interactions were observed in the response of P. fluorescens to all combinations of chlorophenols tested, while the toxicity response of E. coli varied with the matrices tested. Soil characteristics influenced the toxicity response when compared with aqueous solutions. These results highlight the significance of interactive factors and physicochemical parameters when evaluating toxicity. To develop an understanding of pollution derived hazard assessment in soils we need to integrate a wide spectrum of parameters.

Original languageEnglish
Pages (from-to)482-289
Number of pages8
JournalJournal of Environmental Monitoring
Volume4
DOIs
Publication statusPublished - 2002

Keywords

  • ENERGY-TRANSDUCING MEMBRANES
  • CHEMICAL-MIXTURES
  • PENTACHLOROPHENOL
  • SPECIATION
  • EXTRACTION
  • PROPOSAL

Cite this

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title = "Evaluation of interactive toxicity of chlorophenols in water and soil using lux-marked biosensors",
abstract = "An assessment of the toxicity of three chlorophenol compounds (2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP)), individually and in combinations, was made in deionised water, soil extracts and soils using the lux-marked microbial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. These biosensors responded to the bioavailable fraction of pollutants enabling a rapid and ecologically relevant toxicity test. Toxicity interaction responses of pollutant mixtures were predicted after individual compounds were assessed. Synergistic interactions were observed in the response of P. fluorescens to all combinations of chlorophenols tested, while the toxicity response of E. coli varied with the matrices tested. Soil characteristics influenced the toxicity response when compared with aqueous solutions. These results highlight the significance of interactive factors and physicochemical parameters when evaluating toxicity. To develop an understanding of pollution derived hazard assessment in soils we need to integrate a wide spectrum of parameters.",
keywords = "ENERGY-TRANSDUCING MEMBRANES, CHEMICAL-MIXTURES, PENTACHLOROPHENOL, SPECIATION, EXTRACTION, PROPOSAL",
author = "T. Tiensing and Strachan, {Norval James Colin} and Paton, {Graeme Iain}",
year = "2002",
doi = "10.1039/b202070j",
language = "English",
volume = "4",
pages = "482--289",
journal = "Journal of Environmental Monitoring",
issn = "1464-0325",
publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Evaluation of interactive toxicity of chlorophenols in water and soil using lux-marked biosensors

AU - Tiensing, T.

AU - Strachan, Norval James Colin

AU - Paton, Graeme Iain

PY - 2002

Y1 - 2002

N2 - An assessment of the toxicity of three chlorophenol compounds (2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP)), individually and in combinations, was made in deionised water, soil extracts and soils using the lux-marked microbial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. These biosensors responded to the bioavailable fraction of pollutants enabling a rapid and ecologically relevant toxicity test. Toxicity interaction responses of pollutant mixtures were predicted after individual compounds were assessed. Synergistic interactions were observed in the response of P. fluorescens to all combinations of chlorophenols tested, while the toxicity response of E. coli varied with the matrices tested. Soil characteristics influenced the toxicity response when compared with aqueous solutions. These results highlight the significance of interactive factors and physicochemical parameters when evaluating toxicity. To develop an understanding of pollution derived hazard assessment in soils we need to integrate a wide spectrum of parameters.

AB - An assessment of the toxicity of three chlorophenol compounds (2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP)), individually and in combinations, was made in deionised water, soil extracts and soils using the lux-marked microbial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. These biosensors responded to the bioavailable fraction of pollutants enabling a rapid and ecologically relevant toxicity test. Toxicity interaction responses of pollutant mixtures were predicted after individual compounds were assessed. Synergistic interactions were observed in the response of P. fluorescens to all combinations of chlorophenols tested, while the toxicity response of E. coli varied with the matrices tested. Soil characteristics influenced the toxicity response when compared with aqueous solutions. These results highlight the significance of interactive factors and physicochemical parameters when evaluating toxicity. To develop an understanding of pollution derived hazard assessment in soils we need to integrate a wide spectrum of parameters.

KW - ENERGY-TRANSDUCING MEMBRANES

KW - CHEMICAL-MIXTURES

KW - PENTACHLOROPHENOL

KW - SPECIATION

KW - EXTRACTION

KW - PROPOSAL

U2 - 10.1039/b202070j

DO - 10.1039/b202070j

M3 - Article

VL - 4

SP - 482

EP - 289

JO - Journal of Environmental Monitoring

JF - Journal of Environmental Monitoring

SN - 1464-0325

ER -