Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor

Fumio Inagaki, Kai-Uwe Hinrichs, Yusuke Kubo, Marshall W Bowles, Verena B Heuer, Wei-li Hong, Tatsuhiko Hoshino, Akira Ijiri, Hiroyuki Imachi, Motoo Ito, Mark Lever, Masanori Kaneko, Yu-shi Lin, Barbara A Methé, Sumito Morita, Yuki Morono, Wataru Tanikawa, Monika Bihan, Stephen Alan Bowden, Markus Elvert & 26 others Clemens Glombitza, Doris Gross, Guy Harrington, Tomoyukie Hori, Kelvin Li, David Robert Limmer, Chang-Hong Liu, Masafumi Murayama, Naohiko Ohkouchi, Shuhei Ono, Sook-Young Park, Stephen C Phillips, Xavier Prieto-Mollar, Marcella Purkey, Natascha Riedinger, Yoshinori Sanada, Justine Sauvage, Glen Snyder, Rita Susilawati, Yoshinori Takano, Eiji Tasumi, Takeshi Terada, Hitoshi Tomaru, Elizabeth Trembath-Reichert, David T Wang, Yasuhiro Yamada

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Abstract

Microbial populations inhabit deeply buried marine sediments, but the extent of this vast ecosystem is poorly constrained. Here we provide evidence for the existence of microbial communities in sediment associated with lignite coalbeds at ~1.9 to 2.5 km below the seafloor in the Pacific Ocean off Japan. Microbial methanogenesis is indicated by isotopic data of methane and carbon dioxide, methanogenic biomarkers, cultivation and gas composition results. Rigorous protocols aimed at minimizing and correcting for sample contamination resulted in indigenous biomass estimates as low as ~10 cells cm 3 or less in the ~40 to 60°C warm sediments, while coal-bearing sediment layers had elevated cell concentrations. This ultra-deep microbial community is taxonomically distinct from typical shallower deep-biosphere communities found at this and other marine locations.
Original languageEnglish
Pages (from-to)420-424
Number of pages5
JournalScience
Volume349
Issue number6246
DOIs
Publication statusPublished - 24 Jul 2015

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seafloor
coal
microbial community
sediment
methanogenesis
lignite
biosphere
marine sediment
biomarker
carbon dioxide
methane
ecosystem
biomass
ocean
gas
contamination
protocol

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Inagaki, F., Hinrichs, K-U., Kubo, Y., Bowles, M. W., Heuer, V. B., Hong, W., ... Yamada, Y. (2015). Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor. Science, 349(6246), 420-424. https://doi.org/10.1126/science.aaa6882

Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor. / Inagaki, Fumio; Hinrichs, Kai-Uwe; Kubo, Yusuke; Bowles, Marshall W; Heuer, Verena B ; Hong, Wei-li; Hoshino, Tatsuhiko; Ijiri, Akira; Imachi, Hiroyuki; Ito, Motoo; Lever, Mark; Kaneko, Masanori; Lin, Yu-shi; Methé, Barbara A; Morita, Sumito; Morono, Yuki; Tanikawa, Wataru; Bihan, Monika; Bowden, Stephen Alan; Elvert, Markus; Glombitza, Clemens; Gross, Doris; Harrington, Guy; Hori, Tomoyukie; Li, Kelvin; Limmer, David Robert; Liu, Chang-Hong; Murayama, Masafumi; Ohkouchi, Naohiko; Ono, Shuhei; Park, Sook-Young; Phillips, Stephen C; Prieto-Mollar, Xavier; Purkey, Marcella; Riedinger, Natascha; Sanada, Yoshinori; Sauvage, Justine; Snyder, Glen; Susilawati, Rita; Takano, Yoshinori; Tasumi, Eiji; Terada, Takeshi; Tomaru, Hitoshi; Trembath-Reichert, Elizabeth; T Wang, David; Yamada, Yasuhiro.

In: Science, Vol. 349, No. 6246, 24.07.2015, p. 420-424.

Research output: Contribution to journalArticle

Inagaki, F, Hinrichs, K-U, Kubo, Y, Bowles, MW, Heuer, VB, Hong, W, Hoshino, T, Ijiri, A, Imachi, H, Ito, M, Lever, M, Kaneko, M, Lin, Y, Methé, BA, Morita, S, Morono, Y, Tanikawa, W, Bihan, M, Bowden, SA, Elvert, M, Glombitza, C, Gross, D, Harrington, G, Hori, T, Li, K, Limmer, DR, Liu, C-H, Murayama, M, Ohkouchi, N, Ono, S, Park, S-Y, Phillips, SC, Prieto-Mollar, X, Purkey, M, Riedinger, N, Sanada, Y, Sauvage, J, Snyder, G, Susilawati, R, Takano, Y, Tasumi, E, Terada, T, Tomaru, H, Trembath-Reichert, E, T Wang, D & Yamada, Y 2015, 'Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor', Science, vol. 349, no. 6246, pp. 420-424. https://doi.org/10.1126/science.aaa6882
Inagaki F, Hinrichs K-U, Kubo Y, Bowles MW, Heuer VB, Hong W et al. Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor. Science. 2015 Jul 24;349(6246):420-424. https://doi.org/10.1126/science.aaa6882
Inagaki, Fumio ; Hinrichs, Kai-Uwe ; Kubo, Yusuke ; Bowles, Marshall W ; Heuer, Verena B ; Hong, Wei-li ; Hoshino, Tatsuhiko ; Ijiri, Akira ; Imachi, Hiroyuki ; Ito, Motoo ; Lever, Mark ; Kaneko, Masanori ; Lin, Yu-shi ; Methé, Barbara A ; Morita, Sumito ; Morono, Yuki ; Tanikawa, Wataru ; Bihan, Monika ; Bowden, Stephen Alan ; Elvert, Markus ; Glombitza, Clemens ; Gross, Doris ; Harrington, Guy ; Hori, Tomoyukie ; Li, Kelvin ; Limmer, David Robert ; Liu, Chang-Hong ; Murayama, Masafumi ; Ohkouchi, Naohiko ; Ono, Shuhei ; Park, Sook-Young ; Phillips, Stephen C ; Prieto-Mollar, Xavier ; Purkey, Marcella ; Riedinger, Natascha ; Sanada, Yoshinori ; Sauvage, Justine ; Snyder, Glen ; Susilawati, Rita ; Takano, Yoshinori ; Tasumi, Eiji ; Terada, Takeshi ; Tomaru, Hitoshi ; Trembath-Reichert, Elizabeth ; T Wang, David ; Yamada, Yasuhiro. / Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor. In: Science. 2015 ; Vol. 349, No. 6246. pp. 420-424.
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AU - Bowles, Marshall W

AU - Heuer, Verena B

AU - Hong, Wei-li

AU - Hoshino, Tatsuhiko

AU - Ijiri, Akira

AU - Imachi, Hiroyuki

AU - Ito, Motoo

AU - Lever, Mark

AU - Kaneko, Masanori

AU - Lin, Yu-shi

AU - Methé, Barbara A

AU - Morita, Sumito

AU - Morono, Yuki

AU - Tanikawa, Wataru

AU - Bihan, Monika

AU - Bowden, Stephen Alan

AU - Elvert, Markus

AU - Glombitza, Clemens

AU - Gross, Doris

AU - Harrington, Guy

AU - Hori, Tomoyukie

AU - Li, Kelvin

AU - Limmer, David Robert

AU - Liu, Chang-Hong

AU - Murayama, Masafumi

AU - Ohkouchi, Naohiko

AU - Ono, Shuhei

AU - Park, Sook-Young

AU - Phillips, Stephen C

AU - Prieto-Mollar, Xavier

AU - Purkey, Marcella

AU - Riedinger, Natascha

AU - Sanada, Yoshinori

AU - Sauvage, Justine

AU - Snyder, Glen

AU - Susilawati, Rita

AU - Takano, Yoshinori

AU - Tasumi, Eiji

AU - Terada, Takeshi

AU - Tomaru, Hitoshi

AU - Trembath-Reichert, Elizabeth

AU - T Wang, David

AU - Yamada, Yasuhiro

PY - 2015/7/24

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