Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept

Hassan Abbas* (Corresponding Author), Lionel Broche, Aiarpi Ezdoglian, Dmitriy Li, Raif Yuecel, P James Ross, Lesley Cheyne, Heather M Wilson, David J Lurie, Dana K Dawson

*Corresponding author for this work

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Inflammation is central in disease pathophysiology and accurate methods for its detection and quantification are increasingly required to guide diagnosis and therapy. Here we explored the ability of Fast Field-Cycling Magnetic Resonance (FFC-MR) in quantifying the signal of ultra-small superparamagnetic iron oxide particles (USPIO) phagocytosed by J774 macrophage-like cells as a proof-of-principle.
Relaxation rates were measured in suspensions of J774 macrophage-like cells loaded with USPIO (0-200μg/ml Fe as ferumoxytol), using a 0.25 T FFC benchtop relaxometer and a human whole-body, in-house built 0.2 T FFC-MR prototype system with a custom test tube coil. Identical non-imaging, saturation recovery pulse sequence with 90o flip angle and 20 different evolution fields selected logarithmically between 80 μT and 0.2T (3.4kHz and 8.51 MHz proton Larmor frequency [PLF] respectively). Results were compared with imaging flow cytometry quantification of side scatter intensity and USPIO-occupied cell area. A reference colorimetric iron assay was used.
The T1 dispersion curves derived from FFC-MR were excellent in detecting USPIO at all concentrations examined (0-200μg/ml Fe as ferumoxytol) vs. control cells, p ≤ 0.001. FFC-NMR was capable of reliably detecting cellular iron content as low as 1.12ng/µg cell protein, validated using a colorimetric assay. FFC-MR was comparable to imaging flow cytometry quantification of side scatter intensity but superior to USPIO-occupied cell area, the latter being only sensitive at exposures ≥ 10µg/ml USPIO.
We demonstrated for the first time that FFC-MR is capable of quantitative assessment of intra-cellular iron which will have important implications for the use of USPIO in a variety of biological applications, including the study of inflammation.
Original languageEnglish
Article number106722
Number of pages10
JournalJournal of Magnetic Resonance
Early online date26 Mar 2020
Publication statusPublished - Apr 2020


  • fast field-cycling magnetic resonance
  • inflammation
  • ultrasmall superparamagnetic iron oxide particles (USPIO)
  • Fast field-cycling magnetic resonance
  • Ultrasmall superparamagnetic iron oxide particles (USPIO)
  • Inflammation

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Nuclear and High Energy Physics
  • Biophysics
  • Biochemistry

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  • Iain Fraser Cytometry Centre

    Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)

    Institute of Medical Sciences

    Research Facilities: Facility

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