First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities

Tiehui Wang, Petronella Johansson, Beatriz Abós, Amy Holt, Carolina Tafalla, Yousheng Jiang, Alex Wang, Qiaoqing Xu, Qi Zhitao, Wenshu Huang, Maria M. Costa, Patricia Diaz-Rosales, Jason W. Holland, Christopher J. Secombes

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Abstract

IL-4 and IL-13 are closely related canonical type-2 cytokines in mammals and have overlapping bioactivities via shared receptors. They are frequently activated together as part of the same immune response and are the signature cytokines produced by T-helper (Th)2 cells and type-2 innate lymphoid cells (ILC2), mediating immunity against extracellular pathogens. Little is known about the origin of type-2 responses, and whether they were an essential component of the early adaptive immune system that gave a fitness advantage by limiting collateral damage caused by metazoan parasites. Two evolutionary related type-2 cytokines, IL-4/13A and IL-4/13B, have been identified recently in several teleost fish that likely arose by duplication of an ancestral IL-4/13 gene as a consequence of a whole genome duplication event that occurred at the base of this lineage. However, studies of their comparative expression levels are largely missing and bioactivity analysis has been limited to IL-4/13A in zebrafish. Through interrogation of the recently released salmonid genomes, species in which an additional whole genome duplication event has occurred, four genomic IL-4/13 loci have been identified leading to the cloning of three active genes, IL-4/13A, IL-4/13B1 and IL-4/13B2, in both rainbow trout and Atlantic salmon. Comparative expression analysis by real-time PCR in rainbow trout revealed that the IL-4/13A expression is broad and high constitutively but less responsive to pathogen-associated molecular patterns (PAMPs) and pathogen challenge. In contrast, the expression of IL-4/13B1 and IL-4/13B2 is low constitutively but is highly induced by viral haemorrhagic septicaemia virus (VHSH) infection and during proliferative kidney disease (PKD) in vivo, and by formalin-killed bacteria, PAMPs, the T cell mitogen PHA, and the T-cell cytokines IL-2 and IL-21 in vitro. Moreover, bioactive recombinant cytokines of both IL-4/13A and B were produced and found to have shared but also distinct bioactivities. Both cytokines rapidly induce the gene expression of antimicrobial peptides and acute phase proteins, providing an effector mechanism of fish type-2 cytokines in immunity. They are anti-inflammatory via up-regulation of IL-10 and down-regulation of IL-1β and IFN-γ. They modulate the expression of cellular markers of T cells, macrophages and B cells, the receptors of IFN-γ, the IL-6 cytokine family and their own potential receptors, suggesting multiple target cells and important roles of fish type-2 cytokines in the piscine cytokine network. Furthermore both cytokines increased the number of IgM secreting B cells but had no effects on the proliferation of IgM+ B cells in vitro. Taken as a whole, fish IL-4/13A may provide a basal level of type-2 immunity whilst IL-4/13B, when activated, provides an enhanced type-2 immunity, which may have an important role in specific cell-mediated immunity. To our knowledge this is the first in-depth analysis of the expression, modulation and bioactivities of type-2 cytokines in the same fish species, and in any early vertebrate. It contributes to a broader understanding of the evolution of type-2 immunity in vertebrates, and establishes a framework for further studies and manipulation of type-2 cytokines in fish.
Original languageEnglish
Article number7295
Pages (from-to)10917-10946
Number of pages30
JournalOncotarget
Volume7
Issue number10
DOIs
Publication statusPublished - 9 Feb 2016

Fingerprint

Interleukin-4
Fishes
Cytokines
Immunity
Interleukin-13
B-Lymphocytes
Oncorhynchus mykiss
Genome
T-Lymphocytes
Immunoglobulin M
Vertebrates
Novirhabdovirus
Interleukin-6 Receptors
Salmo salar
Th2 Cells
Acute-Phase Proteins
Kidney Diseases
Zebrafish
Virus Diseases
Interleukin-1

Keywords

  • rainbow trout Oncorhynchus mykiss
  • IL-4/13A
  • expression
  • bioactivity
  • type-2 immunity
  • Immunology and Microbiology Section
  • Immune response
  • Immunity
  • IL-4/13B

Cite this

First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities. / Wang, Tiehui; Johansson, Petronella; Abós, Beatriz; Holt, Amy; Tafalla, Carolina; Jiang, Yousheng; Wang, Alex; Xu, Qiaoqing; Zhitao, Qi; Huang, Wenshu; Costa, Maria M.; Diaz-Rosales, Patricia; Holland, Jason W.; Secombes, Christopher J.

In: Oncotarget, Vol. 7, No. 10, 7295, 09.02.2016, p. 10917-10946.

Research output: Contribution to journalArticle

Wang, T, Johansson, P, Abós, B, Holt, A, Tafalla, C, Jiang, Y, Wang, A, Xu, Q, Zhitao, Q, Huang, W, Costa, MM, Diaz-Rosales, P, Holland, JW & Secombes, CJ 2016, 'First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities', Oncotarget, vol. 7, no. 10, 7295, pp. 10917-10946. https://doi.org/10.18632/oncotarget.7295
Wang, Tiehui ; Johansson, Petronella ; Abós, Beatriz ; Holt, Amy ; Tafalla, Carolina ; Jiang, Yousheng ; Wang, Alex ; Xu, Qiaoqing ; Zhitao, Qi ; Huang, Wenshu ; Costa, Maria M. ; Diaz-Rosales, Patricia ; Holland, Jason W. ; Secombes, Christopher J. / First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities. In: Oncotarget. 2016 ; Vol. 7, No. 10. pp. 10917-10946.
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title = "First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities",
abstract = "IL-4 and IL-13 are closely related canonical type-2 cytokines in mammals and have overlapping bioactivities via shared receptors. They are frequently activated together as part of the same immune response and are the signature cytokines produced by T-helper (Th)2 cells and type-2 innate lymphoid cells (ILC2), mediating immunity against extracellular pathogens. Little is known about the origin of type-2 responses, and whether they were an essential component of the early adaptive immune system that gave a fitness advantage by limiting collateral damage caused by metazoan parasites. Two evolutionary related type-2 cytokines, IL-4/13A and IL-4/13B, have been identified recently in several teleost fish that likely arose by duplication of an ancestral IL-4/13 gene as a consequence of a whole genome duplication event that occurred at the base of this lineage. However, studies of their comparative expression levels are largely missing and bioactivity analysis has been limited to IL-4/13A in zebrafish. Through interrogation of the recently released salmonid genomes, species in which an additional whole genome duplication event has occurred, four genomic IL-4/13 loci have been identified leading to the cloning of three active genes, IL-4/13A, IL-4/13B1 and IL-4/13B2, in both rainbow trout and Atlantic salmon. Comparative expression analysis by real-time PCR in rainbow trout revealed that the IL-4/13A expression is broad and high constitutively but less responsive to pathogen-associated molecular patterns (PAMPs) and pathogen challenge. In contrast, the expression of IL-4/13B1 and IL-4/13B2 is low constitutively but is highly induced by viral haemorrhagic septicaemia virus (VHSH) infection and during proliferative kidney disease (PKD) in vivo, and by formalin-killed bacteria, PAMPs, the T cell mitogen PHA, and the T-cell cytokines IL-2 and IL-21 in vitro. Moreover, bioactive recombinant cytokines of both IL-4/13A and B were produced and found to have shared but also distinct bioactivities. Both cytokines rapidly induce the gene expression of antimicrobial peptides and acute phase proteins, providing an effector mechanism of fish type-2 cytokines in immunity. They are anti-inflammatory via up-regulation of IL-10 and down-regulation of IL-1β and IFN-γ. They modulate the expression of cellular markers of T cells, macrophages and B cells, the receptors of IFN-γ, the IL-6 cytokine family and their own potential receptors, suggesting multiple target cells and important roles of fish type-2 cytokines in the piscine cytokine network. Furthermore both cytokines increased the number of IgM secreting B cells but had no effects on the proliferation of IgM+ B cells in vitro. Taken as a whole, fish IL-4/13A may provide a basal level of type-2 immunity whilst IL-4/13B, when activated, provides an enhanced type-2 immunity, which may have an important role in specific cell-mediated immunity. To our knowledge this is the first in-depth analysis of the expression, modulation and bioactivities of type-2 cytokines in the same fish species, and in any early vertebrate. It contributes to a broader understanding of the evolution of type-2 immunity in vertebrates, and establishes a framework for further studies and manipulation of type-2 cytokines in fish.",
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author = "Tiehui Wang and Petronella Johansson and Beatriz Ab{\'o}s and Amy Holt and Carolina Tafalla and Yousheng Jiang and Alex Wang and Qiaoqing Xu and Qi Zhitao and Wenshu Huang and Costa, {Maria M.} and Patricia Diaz-Rosales and Holland, {Jason W.} and Secombes, {Christopher J.}",
note = "ACKNOWLEDGMENTS The VHSV-infected samples were generated within the Scottish Government funded research project FC1996 and kindly provided by Marine Scotland staff. Thanks to ELANCO for providing the A. davidanieli (Renogen). FINANCIAL SUPPORT T. W. received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Y.J., W.H. and Q.X. were supported financially by the National Scholarship Council of China. Z.Q. was supported by grants from the National Natural Science Foundation of China (31302221) and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. M.M.C. was funded by an {\'A}ngeles Alvari{\~n}o postdoctoral contract from the Consejo Superior de Investigaciones Cient{\'i}ficas and the Xunta de Galicia. P.D.-R. was funded by a European Commission (EC) Marie Curie Intra European Fellowship (FP7). J.W.H. was funded by the Biotechnology and Biological Sciences Research Council (BB/K009125/1). This work was also supported financially by the EC, under contract Nos. 222719 (LIFECYCLE) and 311993 (TargetFish), and by the European Research Council Starting Grant 2011 (contract No. 280469).",
year = "2016",
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TY - JOUR

T1 - First in-depth analysis of the novel Th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities

AU - Wang, Tiehui

AU - Johansson, Petronella

AU - Abós, Beatriz

AU - Holt, Amy

AU - Tafalla, Carolina

AU - Jiang, Yousheng

AU - Wang, Alex

AU - Xu, Qiaoqing

AU - Zhitao, Qi

AU - Huang, Wenshu

AU - Costa, Maria M.

AU - Diaz-Rosales, Patricia

AU - Holland, Jason W.

AU - Secombes, Christopher J.

N1 - ACKNOWLEDGMENTS The VHSV-infected samples were generated within the Scottish Government funded research project FC1996 and kindly provided by Marine Scotland staff. Thanks to ELANCO for providing the A. davidanieli (Renogen). FINANCIAL SUPPORT T. W. received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland). MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. Y.J., W.H. and Q.X. were supported financially by the National Scholarship Council of China. Z.Q. was supported by grants from the National Natural Science Foundation of China (31302221) and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. M.M.C. was funded by an Ángeles Alvariño postdoctoral contract from the Consejo Superior de Investigaciones Científicas and the Xunta de Galicia. P.D.-R. was funded by a European Commission (EC) Marie Curie Intra European Fellowship (FP7). J.W.H. was funded by the Biotechnology and Biological Sciences Research Council (BB/K009125/1). This work was also supported financially by the EC, under contract Nos. 222719 (LIFECYCLE) and 311993 (TargetFish), and by the European Research Council Starting Grant 2011 (contract No. 280469).

PY - 2016/2/9

Y1 - 2016/2/9

N2 - IL-4 and IL-13 are closely related canonical type-2 cytokines in mammals and have overlapping bioactivities via shared receptors. They are frequently activated together as part of the same immune response and are the signature cytokines produced by T-helper (Th)2 cells and type-2 innate lymphoid cells (ILC2), mediating immunity against extracellular pathogens. Little is known about the origin of type-2 responses, and whether they were an essential component of the early adaptive immune system that gave a fitness advantage by limiting collateral damage caused by metazoan parasites. Two evolutionary related type-2 cytokines, IL-4/13A and IL-4/13B, have been identified recently in several teleost fish that likely arose by duplication of an ancestral IL-4/13 gene as a consequence of a whole genome duplication event that occurred at the base of this lineage. However, studies of their comparative expression levels are largely missing and bioactivity analysis has been limited to IL-4/13A in zebrafish. Through interrogation of the recently released salmonid genomes, species in which an additional whole genome duplication event has occurred, four genomic IL-4/13 loci have been identified leading to the cloning of three active genes, IL-4/13A, IL-4/13B1 and IL-4/13B2, in both rainbow trout and Atlantic salmon. Comparative expression analysis by real-time PCR in rainbow trout revealed that the IL-4/13A expression is broad and high constitutively but less responsive to pathogen-associated molecular patterns (PAMPs) and pathogen challenge. In contrast, the expression of IL-4/13B1 and IL-4/13B2 is low constitutively but is highly induced by viral haemorrhagic septicaemia virus (VHSH) infection and during proliferative kidney disease (PKD) in vivo, and by formalin-killed bacteria, PAMPs, the T cell mitogen PHA, and the T-cell cytokines IL-2 and IL-21 in vitro. Moreover, bioactive recombinant cytokines of both IL-4/13A and B were produced and found to have shared but also distinct bioactivities. Both cytokines rapidly induce the gene expression of antimicrobial peptides and acute phase proteins, providing an effector mechanism of fish type-2 cytokines in immunity. They are anti-inflammatory via up-regulation of IL-10 and down-regulation of IL-1β and IFN-γ. They modulate the expression of cellular markers of T cells, macrophages and B cells, the receptors of IFN-γ, the IL-6 cytokine family and their own potential receptors, suggesting multiple target cells and important roles of fish type-2 cytokines in the piscine cytokine network. Furthermore both cytokines increased the number of IgM secreting B cells but had no effects on the proliferation of IgM+ B cells in vitro. Taken as a whole, fish IL-4/13A may provide a basal level of type-2 immunity whilst IL-4/13B, when activated, provides an enhanced type-2 immunity, which may have an important role in specific cell-mediated immunity. To our knowledge this is the first in-depth analysis of the expression, modulation and bioactivities of type-2 cytokines in the same fish species, and in any early vertebrate. It contributes to a broader understanding of the evolution of type-2 immunity in vertebrates, and establishes a framework for further studies and manipulation of type-2 cytokines in fish.

AB - IL-4 and IL-13 are closely related canonical type-2 cytokines in mammals and have overlapping bioactivities via shared receptors. They are frequently activated together as part of the same immune response and are the signature cytokines produced by T-helper (Th)2 cells and type-2 innate lymphoid cells (ILC2), mediating immunity against extracellular pathogens. Little is known about the origin of type-2 responses, and whether they were an essential component of the early adaptive immune system that gave a fitness advantage by limiting collateral damage caused by metazoan parasites. Two evolutionary related type-2 cytokines, IL-4/13A and IL-4/13B, have been identified recently in several teleost fish that likely arose by duplication of an ancestral IL-4/13 gene as a consequence of a whole genome duplication event that occurred at the base of this lineage. However, studies of their comparative expression levels are largely missing and bioactivity analysis has been limited to IL-4/13A in zebrafish. Through interrogation of the recently released salmonid genomes, species in which an additional whole genome duplication event has occurred, four genomic IL-4/13 loci have been identified leading to the cloning of three active genes, IL-4/13A, IL-4/13B1 and IL-4/13B2, in both rainbow trout and Atlantic salmon. Comparative expression analysis by real-time PCR in rainbow trout revealed that the IL-4/13A expression is broad and high constitutively but less responsive to pathogen-associated molecular patterns (PAMPs) and pathogen challenge. In contrast, the expression of IL-4/13B1 and IL-4/13B2 is low constitutively but is highly induced by viral haemorrhagic septicaemia virus (VHSH) infection and during proliferative kidney disease (PKD) in vivo, and by formalin-killed bacteria, PAMPs, the T cell mitogen PHA, and the T-cell cytokines IL-2 and IL-21 in vitro. Moreover, bioactive recombinant cytokines of both IL-4/13A and B were produced and found to have shared but also distinct bioactivities. Both cytokines rapidly induce the gene expression of antimicrobial peptides and acute phase proteins, providing an effector mechanism of fish type-2 cytokines in immunity. They are anti-inflammatory via up-regulation of IL-10 and down-regulation of IL-1β and IFN-γ. They modulate the expression of cellular markers of T cells, macrophages and B cells, the receptors of IFN-γ, the IL-6 cytokine family and their own potential receptors, suggesting multiple target cells and important roles of fish type-2 cytokines in the piscine cytokine network. Furthermore both cytokines increased the number of IgM secreting B cells but had no effects on the proliferation of IgM+ B cells in vitro. Taken as a whole, fish IL-4/13A may provide a basal level of type-2 immunity whilst IL-4/13B, when activated, provides an enhanced type-2 immunity, which may have an important role in specific cell-mediated immunity. To our knowledge this is the first in-depth analysis of the expression, modulation and bioactivities of type-2 cytokines in the same fish species, and in any early vertebrate. It contributes to a broader understanding of the evolution of type-2 immunity in vertebrates, and establishes a framework for further studies and manipulation of type-2 cytokines in fish.

KW - rainbow trout Oncorhynchus mykiss

KW - IL-4/13A

KW - expression

KW - bioactivity

KW - type-2 immunity

KW - Immunology and Microbiology Section

KW - Immune response

KW - Immunity

KW - IL-4/13B

U2 - 10.18632/oncotarget.7295

DO - 10.18632/oncotarget.7295

M3 - Article

C2 - 26870894

VL - 7

SP - 10917

EP - 10946

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 10

M1 - 7295

ER -