Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1

T Toda, M Shimanuki, Yasushi Saka, H Yamano, Y Adachi, M Shirakawa, Y Kyogoku, M Yanagida

Research output: Contribution to journalArticle

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Abstract

The fission yeast pap1+ gene encodes an AP-1-like transcription factor that contains a leucine zipper motif. We identified a target gene of pap1, the p25 gene. The 5' upstream region of the p25 gene contains an AP-1 site, and by DNase I footprint analysis, we showed that the pap1 protein binds to the AP-1 site as well as to a 14-bp palindrome sequence. p25 is overproduced when the pap1+ gene is overexpressed, whereas p25 is not produced at all in the pap1 deletion mutant. p25 was previously found to be overproduced in strains carrying cold-sensitive crm1 mutations whose gene product is essential for viability and is thought to play an important role in maintenance of a proper chromosomal architecture. Deletion and site-directed mutagenesis of sequences upstream of the p25 gene demonstrated that the AP-1 site as well as the palindrome sequence are crucial for transcriptional activation either by pap1 overproduction or by the cold-sensitive crm1 mutation; pap1+ is apparently negatively regulated by crm1+. Moreover, we found that cold-sensitive crm1 mutations are suppressed by the deletion of pap1+, further indicating a close relationship between crm1+ and pap1+. The crm1 protein is highly conserved; the budding yeast homolog, CRM1, which complements the fission yeast cold-sensitive crm1 mutation, was isolated and found to also be essential for viability. These results suggest the functional importance of chromosome structure on the regulation of gene expression through the pap1 transcription factor.
Original languageEnglish
Pages (from-to)5474-5484
Number of pages11
JournalMolecular and Cellular Biology
Volume12
Issue number12
Publication statusPublished - 1 Dec 1992

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Schizosaccharomyces
Nuclear Proteins
Transcription Factor AP-1
Genes
Inverted Repeat Sequences
Mutation
Chromosome Structures
Leucine Zippers
Saccharomycetales
Sequence Deletion
Deoxyribonuclease I
Gene Expression Regulation
Site-Directed Mutagenesis
Transcriptional Activation
Proteins
Transcription Factors
Maintenance

Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Binding Sites
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Karyopherins
  • Molecular Sequence Data
  • Proto-Oncogene Proteins c-jun
  • Receptors, Cytoplasmic and Nuclear
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid
  • Transcription Factors
  • Transcription, Genetic

Cite this

Toda, T., Shimanuki, M., Saka, Y., Yamano, H., Adachi, Y., Shirakawa, M., ... Yanagida, M. (1992). Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. Molecular and Cellular Biology, 12(12), 5474-5484.

Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. / Toda, T; Shimanuki, M; Saka, Yasushi; Yamano, H; Adachi, Y; Shirakawa, M; Kyogoku, Y; Yanagida, M.

In: Molecular and Cellular Biology, Vol. 12, No. 12, 01.12.1992, p. 5474-5484.

Research output: Contribution to journalArticle

Toda, T, Shimanuki, M, Saka, Y, Yamano, H, Adachi, Y, Shirakawa, M, Kyogoku, Y & Yanagida, M 1992, 'Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1', Molecular and Cellular Biology, vol. 12, no. 12, pp. 5474-5484.
Toda T, Shimanuki M, Saka Y, Yamano H, Adachi Y, Shirakawa M et al. Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. Molecular and Cellular Biology. 1992 Dec 1;12(12):5474-5484.
Toda, T ; Shimanuki, M ; Saka, Yasushi ; Yamano, H ; Adachi, Y ; Shirakawa, M ; Kyogoku, Y ; Yanagida, M. / Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 12. pp. 5474-5484.
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abstract = "The fission yeast pap1+ gene encodes an AP-1-like transcription factor that contains a leucine zipper motif. We identified a target gene of pap1, the p25 gene. The 5' upstream region of the p25 gene contains an AP-1 site, and by DNase I footprint analysis, we showed that the pap1 protein binds to the AP-1 site as well as to a 14-bp palindrome sequence. p25 is overproduced when the pap1+ gene is overexpressed, whereas p25 is not produced at all in the pap1 deletion mutant. p25 was previously found to be overproduced in strains carrying cold-sensitive crm1 mutations whose gene product is essential for viability and is thought to play an important role in maintenance of a proper chromosomal architecture. Deletion and site-directed mutagenesis of sequences upstream of the p25 gene demonstrated that the AP-1 site as well as the palindrome sequence are crucial for transcriptional activation either by pap1 overproduction or by the cold-sensitive crm1 mutation; pap1+ is apparently negatively regulated by crm1+. Moreover, we found that cold-sensitive crm1 mutations are suppressed by the deletion of pap1+, further indicating a close relationship between crm1+ and pap1+. The crm1 protein is highly conserved; the budding yeast homolog, CRM1, which complements the fission yeast cold-sensitive crm1 mutation, was isolated and found to also be essential for viability. These results suggest the functional importance of chromosome structure on the regulation of gene expression through the pap1 transcription factor.",
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AB - The fission yeast pap1+ gene encodes an AP-1-like transcription factor that contains a leucine zipper motif. We identified a target gene of pap1, the p25 gene. The 5' upstream region of the p25 gene contains an AP-1 site, and by DNase I footprint analysis, we showed that the pap1 protein binds to the AP-1 site as well as to a 14-bp palindrome sequence. p25 is overproduced when the pap1+ gene is overexpressed, whereas p25 is not produced at all in the pap1 deletion mutant. p25 was previously found to be overproduced in strains carrying cold-sensitive crm1 mutations whose gene product is essential for viability and is thought to play an important role in maintenance of a proper chromosomal architecture. Deletion and site-directed mutagenesis of sequences upstream of the p25 gene demonstrated that the AP-1 site as well as the palindrome sequence are crucial for transcriptional activation either by pap1 overproduction or by the cold-sensitive crm1 mutation; pap1+ is apparently negatively regulated by crm1+. Moreover, we found that cold-sensitive crm1 mutations are suppressed by the deletion of pap1+, further indicating a close relationship between crm1+ and pap1+. The crm1 protein is highly conserved; the budding yeast homolog, CRM1, which complements the fission yeast cold-sensitive crm1 mutation, was isolated and found to also be essential for viability. These results suggest the functional importance of chromosome structure on the regulation of gene expression through the pap1 transcription factor.

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