Abstract
The introduction of fluorine atoms into organic molecules has received considerable attentions as these organofluorines have often found widespread applications in bioorganic chemistry, medicinal chemistry and
biomaterial science. Despite innovation of synthetic C–F forming methodologies, selective fluorination is still extremely challenging. Therefore, a biotransformation approach using fluorine biocatalysts is needed to selectively
introduce fluorine into structurally diverse molecules. Yet, there are few ways that enable incorporation of fluorine into structurally complex bioactive molecules. One is to extend the substrate scope of the existing enzyme
inventory. Another is to expand the biosynthetic pathways to accept fluorinated precursors for producing fluorinated bioactive molecules. Finally, an understanding of the physiological roles of fluorometabolites in the
producing microorganisms will advance our ability to engineer a microorganism to produce novel fluorinated commodities. Here, we review the fluorinase biotechnology and fluorine biocatalysts that incorporate fluorine
motifs to generate fluorinated molecules, and highlight areas for future developments.
biomaterial science. Despite innovation of synthetic C–F forming methodologies, selective fluorination is still extremely challenging. Therefore, a biotransformation approach using fluorine biocatalysts is needed to selectively
introduce fluorine into structurally diverse molecules. Yet, there are few ways that enable incorporation of fluorine into structurally complex bioactive molecules. One is to extend the substrate scope of the existing enzyme
inventory. Another is to expand the biosynthetic pathways to accept fluorinated precursors for producing fluorinated bioactive molecules. Finally, an understanding of the physiological roles of fluorometabolites in the
producing microorganisms will advance our ability to engineer a microorganism to produce novel fluorinated commodities. Here, we review the fluorinase biotechnology and fluorine biocatalysts that incorporate fluorine
motifs to generate fluorinated molecules, and highlight areas for future developments.
Original language | English |
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Pages (from-to) | 119-126 |
Number of pages | 6 |
Journal | Current Opinion in Chemical Biology |
Volume | 55 |
Early online date | 19 Feb 2020 |
DOIs | |
Publication status | Published - Apr 2020 |
Bibliographical note
HD is grateful for the financial supports through Biotechnology and Biological Sciences Research Council (BBSRC) grants (BB/P00380X/1 and BB/R00479X/1) and Leverhulme Trust (RPG- 2014-418). HD and LW thank Industrial Biotechnology Innovation Centre (IBioIC) for the PhD studentship (LW). FM thanks the University of the Philippines for the Faculty, Reps and Staff Development Program (FRAS DP) PhD grant fellowship.Keywords
- BB/P00380X/1
- BB/R00479X/1
- FLUOROMETABOLITE BIOSYNTHESIS
- RGD PEPTIDE
- MECHANISM
- METABOLISM
- ENZYMATIC FLUORINATION
- BOND FORMATION
- STREPTOMYCES SP MA37
- IDENTIFICATION
- F-18
- ANTIBIOTIC NUCLEOCIDIN