Gabaergic projections from the medial septum selectively inhibit interneurons in the medial entorhinal cortex

A. Gonzalez-Sulser; D. Parthier; A. Candela; C. McClure; H. Pastoll; D. Garden; G. Sürmeli; M.F. Nolan

doi:10.1523/JNEUROSCI.1612-14.2014

Gabaergic projections from the medial septum selectively inhibit interneurons in the medial entorhinal cortex

A. Gonzalez-Sulser, D. Parthier, A. Candela, C. McClure, H. Pastoll, D. Garden, G. Sürmeli, M.F. Nolan

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

The medial septum (MS) is required for theta rhythmic oscillations and grid cell firing in the medial entorhinal cortex (MEC). While GABAergic, glutamatergic, and cholinergic neurons project from the MS to the MEC, their synaptic targets are unknown. To investigate whether MS neurons innervate specific layers and cell types in the MEC, we expressed channelrhodopsin-2 in mouse MS neurons and used patch-clamp recording in brain slices to determine the response to light activation of identified cells in the MEC. Following activation of MS axons, we observed fast monosynaptic GABAergic IPSPs in the majority (>60%) of fast-spiking (FS) and low-threshold-spiking (LTS) interneurons in all layers of the MEC, but in only 1.5% of nonstellate principal cells (NSPCs) and in no stellate cells. We also observed fast glutamatergic responses to MS activation in a minority (

Original language	English
Pages (from-to)	16739-16743
Journal	Journal of Neuroscience
Volume	34
Issue number	50
DOIs	https://doi.org/10.1523/JNEUROSCI.1612-14.2014
Publication status	Published - 10 Dec 2014

Keywords

gamma
interneuron
lamina organization
medial entorhinal cortex
medial septum
theta

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Gonzalez-Sulser_etal_TJN_GABAergic_Projections_From_VOR
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Cite this

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title = "Gabaergic projections from the medial septum selectively inhibit interneurons in the medial entorhinal cortex",

abstract = "The medial septum (MS) is required for theta rhythmic oscillations and grid cell firing in the medial entorhinal cortex (MEC). While GABAergic, glutamatergic, and cholinergic neurons project from the MS to the MEC, their synaptic targets are unknown. To investigate whether MS neurons innervate specific layers and cell types in the MEC, we expressed channelrhodopsin-2 in mouse MS neurons and used patch-clamp recording in brain slices to determine the response to light activation of identified cells in the MEC. Following activation of MS axons, we observed fast monosynaptic GABAergic IPSPs in the majority (>60%) of fast-spiking (FS) and low-threshold-spiking (LTS) interneurons in all layers of the MEC, but in only 1.5% of nonstellate principal cells (NSPCs) and in no stellate cells. We also observed fast glutamatergic responses to MS activation in a minority (",

keywords = "gamma, interneuron, lamina organization, medial entorhinal cortex, medial septum, theta",

author = "A. Gonzalez-Sulser and D. Parthier and A. Candela and C. McClure and H. Pastoll and D. Garden and G. S{\"u}rmeli and M.F. Nolan",

note = "This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC Grants BB/L010496/1 and BB/H020284/1 to M.F.N.), a Marie-Curie International Incoming Post-Doctoral Fellowship (629586 to A.G.-S.), and a Sir Henry Wellcome Postdoctoral Fellowship (098915/Z/12/z to G.S.). We thank the IMPACT facility at the University of Edinburgh for imaging resources.",

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AU - Gonzalez-Sulser, A.

AU - Parthier, D.

AU - Candela, A.

AU - McClure, C.

AU - Pastoll, H.

AU - Garden, D.

AU - Sürmeli, G.

AU - Nolan, M.F.

N1 - This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC Grants BB/L010496/1 and BB/H020284/1 to M.F.N.), a Marie-Curie International Incoming Post-Doctoral Fellowship (629586 to A.G.-S.), and a Sir Henry Wellcome Postdoctoral Fellowship (098915/Z/12/z to G.S.). We thank the IMPACT facility at the University of Edinburgh for imaging resources.

PY - 2014/12/10

Y1 - 2014/12/10

N2 - The medial septum (MS) is required for theta rhythmic oscillations and grid cell firing in the medial entorhinal cortex (MEC). While GABAergic, glutamatergic, and cholinergic neurons project from the MS to the MEC, their synaptic targets are unknown. To investigate whether MS neurons innervate specific layers and cell types in the MEC, we expressed channelrhodopsin-2 in mouse MS neurons and used patch-clamp recording in brain slices to determine the response to light activation of identified cells in the MEC. Following activation of MS axons, we observed fast monosynaptic GABAergic IPSPs in the majority (>60%) of fast-spiking (FS) and low-threshold-spiking (LTS) interneurons in all layers of the MEC, but in only 1.5% of nonstellate principal cells (NSPCs) and in no stellate cells. We also observed fast glutamatergic responses to MS activation in a minority (

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Gabaergic projections from the medial septum selectively inhibit interneurons in the medial entorhinal cortex

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